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Mariola Plazas, Santiago Vilanova, Pietro Gramazio, Adrián Rodríguez-Burruezo, Ana Fita, Francisco J. Herraiz, Rajakapasha Ranil, Ramya Fonseka, Lahiru Niran, Hemal Fonseka, Brice Kouassi, Abou Kouassi, Auguste Kouassi and Jaime Prohens

interspecific hybridization is the direction of the cross, as this may affect its rate of success, the number of seeds produced, as well as the dormancy of the seeds due to maternal effects ( Morgan et al., 2010 ). In this respect, using S. melongena as the

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Randall J. Marek and Ronald D. Parker

An interspecific hybridization program involving ancestral species of the Begonia Semperflorens Cultorum Group was initiated to expand the genetic base of this group. Viable seeds were recovered from four reciprocal crosses. F, progenies were sterile and phenotypically intermediate between parental types. Fluorescence microscopy revealed evidence of both sporophytic and gametophytic incompatibility. Post-pollnation responses of flower petals were positively correlated with pollen tube growth in stigmatic, stylar, and ovarian tissue. A digital image analyzer was used to facilitate seed counts and to determine the percentage of ovules that developed into seeds. Seed germination percentages ranged from 0-91 for crosses to 80-99 for selfs.

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Chunqing Sun, Zhihu Ma, Zhenchao Zhang, Guosheng Sun and Zhongliang Dai

lilies. To obtain desired traits of interest in cultivars, breeders have tried to make interspecific hybridizations. In the past, interspecific hybridizations in water lily have been performed for the hope of creating a blue hardy water lily hybrid, but

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Maryke A. Cleland, Cynthia Jones and Mark H. Brand

An interspecific hybridization program involving five species of Impatiens was initiated to delineate incompatibility barriers. With the exception of one cross, no viable hybrid seed was recovered. Fluorescence microscopy revealed foreign pollen tubes to reach ovules in all crosses, although not all ovules were approached. A histological study involving I. auricoma Baill. and I. walleriana Hook f. ensued to confirm the presence of hybrid embryos. Developing I. walleriana × I. auricoma and reciprocal hybrid embryos were compared to self embryos. Development of hybrid embryos was delayed as early as five days post-pollination. I. walleriana × I. auricoma embryos continued to develop for 8 days post-pollination, but did not reach a size greater than a 5-day self embryo. Excessive endosperm was observed in the hybrid. I. auricoma × I. walleriana embryos continued to enlarge up to ovary abortion but did not reach a size greater than a 7-day self embryo and little to no endosperm developed. Disintegration of ovules included disorganization and collapse of the endosperm, and vacuolization and loss of turgidity of the embryo.

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Margaret R. Pooler and Ruth L. Dix

Interspecific hybridizations among members of the genus Hamamelis (the witchhazels) and Corylopsis were carried out in 1993, 1994, 1995, and 1996 at the U.S. National Arboretum. Specifically, crosses involving the native witchhazel (H. vernalis and H. virginiana) and the Asian taxa (H. mollis, H. japonica, and H. × intermedia) were attempted in order to combine the ornamental qualities of the Asian species with the adaptability and fall blooming characteristics of the native species. Additionally, C. platypetala, a hardy species with small inflorescences, was crossed with C. himalaica, which has large showy inflorescences but is less hardy. Approximately 50 seedlings resulting from these crosses have been analyzed using randomly amplified polymorphic DNA (RAPD) markers to verify interspecific hybridization. Based on these assays, we report the first incidence of controlled interspecific hybridization between the Asian and native witchhazel taxa.

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Sarah M. Smith and Zhanao Deng

plant species are brought into close proximity for seed production or planting, interspecific hybridization can occur ( Ellstrand, 1992 ). Interspecific hybridization could lead to genetic contamination of native wildflower seed being produced. If

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Nicholi Vorsa and James J. Polashock

The flavonoids of american cranberry (Vaccinium macrocarpon Ait.) are documented to be beneficial for human health. Among their benefits is a high antioxidant potential, with anthocyanin glycosides being the main contributors. Flavonoid glucose conjugates are reported to be more bioavailable than those with other sugar conjugates. The anthocyanin glycosides of V. macrocarpon fruit are mainly galactosides and arabinosides of the aglycones, cyanidin and peonidin, with less than 8% glucosides. In contrast, the fruit anthocyanins of another cranberry species, V. oxycoccus L. were found to be largely glucosides of cyanidin and peonidin. Interspecific hybrids between these two species were intermediate to the parental species in the proportion of fruit anthocyanin glucosides. About half the progeny (1:1 segregation) in a backcross population (to V. macrocarpon) maintained the relatively high anthocyanin glucoside ratio. In this study, we demonstrate the genetic manipulation of anthocyanin glycosylation in cranberry using interspecific hybridization, resulting in dramatically increased glucose-conjugated anthocyanins.

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William A. Hoch, Geunhwa Jung and Brent H. McCown

A significant pest affecting commonly planted Betula spp. is the birch leafminer (Fenusa pusilla Lepeletier), an insect that can be present in large populations in the landscape and can greatly reduce the vigor and ornamental value of these trees. Twenty-two interspecific crosses were performed between leafminer resistant and susceptible Betula species in an attempt to create the novel combination of ornamental white bark and significant leafminer resistance. Of the nine successful crosses, two produced resistant offspring. Progeny of the diploid × hexaploid cross B. turkestanica Litvin (2x) × B. alleghaniensis Britt. (6x) displayed a broad range of resistance levels, likely the result of segregating alleles contributed by the hexaploid parent. All crosses involving highly resistant individuals of B. costata Trautv. (2x) yielded leafminer susceptible progeny. These results suggest that the larval antibiosis demonstrated by B. alleghaniensis and B. costata is inherited as a recessive trait, and exhibits a gene dosage effect as evidenced by the B. turkestanica × B. alleghaniensis offspring. While most progeny of the B. populifolia Marsh (2x) × B. maximowicziana Regal (2x) cross were susceptible, a single resistant offspring, which was found to be triploid (3x), displayed a mechanism of resistance similar to that of a hypersensitive response. No strong intersectional barriers to hybridization were observed and all interploidy crosses were successful. The chromosome numbers of B. costata (2n = 2x = 28) and B. turkestanica (2n = 2x = 28) are reported here for the first time. The results of this study indicate that the potential exists for the development of insect resistant, ornamental white-barked birch clones through the implementation of a planned, systematic breeding program.

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R.H. Bors and J.A. Sullivan

The potential of using Fragaria vesca L. as a bridge species for interspecific hybridization to F. nilgerrensis Schlect, F. nubicola Lindl., F. pentaphylla Losinsk, and F. viridis Duch. was investigated using a wide germplasm base of 40 F. vesca accessions. This study was successful in producing many hybrids between F. vesca and other diploid species indicating its value as a bridge species. Of the species used as males, F. nubicola, F. pentaphylla, and F. viridis accessions were more successful, averaging 8 to 16 fruit and 16 to 25 seeds/fruit. It was most difficult to obtain hybrids with F. nilgerrensis, which had only three seeds per fruit. Differences among pollen donors were minimal when hybrid seeds were germinated in vitro. For different species combinations, 75% to 99% of seeds had embryos and 77% to 89% of these embryos germinated. The lack of significant differences in crossability variables among the four F. vesca subspecies [i.e., ssp. americana (Porter) Staudt, ssp. bracteata (Heller) Staudt, ssp. vesca L., and ssp. vesca var. semper-florens L.] demonstrated the similarity between these species and the strong potential for gene flow between F. vesca and other diploid species. As European and North American F. vesca subspecies are not sufficiently divergent to differ in interspecific hybridization, F. vesca may be a younger species rather than an older progenitor species.

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Yasumasa Takatsu, Masakazu Kasumi, Toru Manabe, Mikio Hayashi, Eiichi Inoue, Wataru Marubashi and Masaru Niwa

Interspecific hybridization between a modern cultivar of Gladiolu×grandiflora hort. (2n = 60) and the wild species G. tristis L. (2n = 30) was made to introduce characteristics of the wild species into the cultivated one. Gladiolus ×grandiflora is a summer-flowering species, and G. tristis flowers in winter. The effect of storage temperature on pollen viability was tested, as long-term storage of pollen was necessary to facilitate crossing these two species. Pollen of G. tristis could be stored at -20 °C for ≈1 year, and was more practical than storage at -80 °C. Air temperature affected pollen tube growth, fertility, and fruit set in the cross between G. ×grandiflora and G. tristis, and low temperatures (15 to 20 °C) were best. The morphological data and flow cytometric analysis showed that the F1 plants were hybrids between G. ×grandiflora and G. tristis.