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Dean E. Knavel

Abbreviations: NI, normal internode; SI, short internode. This Kentucky Agricultural Experiment Station paper (90-10-168) is published by permission of the Director. The cost of publishing this paper was defrayed in part by the payment of page

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Shuguang Wang, Yongpeng Ma, Chengbin Wan, Chungyun Hse, Todd F. Shupe, Yujun Wang, and Changming Wang

this paper, the spatial distribution patterns of endogenous IAA and ABA, and their dynamic changes during shoot elongation of Fargesia yunnanensis were investigated. The correlation between phytohormones and bamboo internode elongation was also

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Taifeng Zhang, Jiajun Liu, Shi Liu, Zhuo Ding, Feishi Luan, and Peng Gao

advantages, including greater planting density and resistance to storm and lodging, and reduced losses during harvesting operations (Amasino et al., 2003). Many dwarf crop traits, including short internodes, short MSL, and bush-type growth habits, have been

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Nguyen Phuc Huy, Vu Quoc Luan, Le Kim Cuong, Nguyen Ba Nam, Hoang Thanh Tung, Vu Thi Hien, Dung Tien Le, Kee Yoeup Paek, and Duong Tan Nhut

-elongated ex vitro explants as the source under dark–light cycles for plant regeneration through internode tissue cultures. The results of this study provide a new approach to micropropagation of P. callosum for commercial propagation. Materials and Methods

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Ilse-Yazmín Arciniega-Carreón, Carmen Oliver-Salvador, María-Guadalupe Ramírez-Sotelo, and Carlos Edmundo Salas

metabolites as well. Materials and Methods Plant material. I. sonorae (S. Watson) Greene plants (≈750 g) were obtained at the local Sonora market, Mexico City, Mexico. The plant internode containing axillary buds (NXB) was selected as the source of explants

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Jim Hruskoci and Paul E. Read

In an effort to increase somaclonal variation in blueberry, a protocol was developed to regenerate viable shoots from internode segments. The explant consisted of the last-formed, fully developed internode taken from 3 different genotypes of Vaccinium grown in vitro. Explants were cultured 6 weeks on Zimmerman's Z-2 medium supplemented with 2iP, zeatin, thidiazuron, kinetin, or BA at concentrations of 5, 25, 50, and 100 uM. Explant response to the treatments varied and included: no response, callus growth only, callus growth and subsequent shoot formation originating from the callus mass, and adventitious shoot formation directly from the internode segment without an intervening callus. Greatest shoot regeneration (20-25 shoots/explant) was obtained on medium supplemented with zeatin at 5, 25, and 50 uM, however treatment response was not consistant across all genotypes. Regenerated shoots could be readily sub-cultured, rooted in soil mix and will be evaluated for somaclonal variation.

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Dean E. Knavel and Robert L. Houtz

Plants of Main Dwarf, a short-internode mutant of the normal-internode `Mainstream' muskmelon (Cucumis melo L.), have shorter internodes, fewer nodes, less total vine length, less total dry weight, smaller leaves, increased chlorophyll concentrations, increased specific leaf dry weight, and increased ribulose-1, 5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39, rubisco) activity per unit leaf area than `Mainstream' plants. Main Dwarf plants produce an equal number of fruit as `Mainstream' plants but are only half their size. Many of the plant and fruit characteristics for F1(Main Dwarf × `Mainstream') are similar to those of `Mainstream', except for greater leaf chlorophyll and rubisco activity per unit leaf area. The F1 (`Mainstream' × Main Dwarf) produced fewer and lower weight fruit than its reciprocal, F1 (Main Dwarf × `Mainstream').

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W. Garrett Owen and Roberto G. Lopez

composition, IBA application, and rooting success at different node positions. To our knowledge, no studies have been published on the effects of PDLI and RZT on rhizogenesis of vegetative single-internode purple fountain grass culm cuttings. Therefore, our

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Dean E. Knavel

A short-internode mutant of `Mainstream' muskmelon (Cucumis melo L.), designated Main Dwarf, was crossed with 13 normal and six short-internode cultivars or breeding lines. Regardless of whether Main Dwarf was crossed with a normal or short-internode line, the F1 family was normal for internode length. From crosses of Main Dwarf with normal lines, the F2 families segregated in a 3 normal: 1 short-internode ratio and the families from backcrosses to Main Dwarf segregated 1 normal: 1 short internode. Crosses of Main Dwarf with short-internode lines produced F2 families that segregated in a 9 normal: 7 short internode ratio and families from backcrosses to Main Dwarf that segregated 1 normal: 1 short internode. The segregation data from crosses of Main Dwarf with normal lines indicate that Main Dwarf has a recessive gene that conditions short internode. The segregation data from crosses of Main Dwarf with short-internode lines (five conditioned by si-1 and one conditioned by si-2) indicate that the recessive gene for short internode in Main Dwarf is not allelic to si-1 or si-2. The gene for short internodes in Main Dwarf is designated si-3.

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Fure-Chyi Chen and Adelheid R. Kuehnle

Several procedures were tested in development of a gene transfer protocol for anthurium. Etiolated internode segments of anthurium cultivars `Rudolph' and `UH1060' were co-cultured with Agrobacterium tumefaciens LBA4404 carrying the chimeric genes neo, for antibiotic resistance, and att encoding antibacterial attacin. Assays of genomic DNA and RNA from kanamycin-resistant `Rudolph' and DNA from `UH1060' plantlets, recovered as soon as 1 year after culture on selection media, indicated the presence of introduced genes, including neo and att, and transcription of att. Western analysis confirmed the expression of attacin protein in calli induced from laminae of regenerated kanamycinresistant `Rudolph' plantlets. Use of tobacco nurse cells during co-cultivation of internodes with Agrobacterium did not increase recovery of shoots under the regeneration conditions used. Improvements in culture and antibiotic selection conditions during plant development are suggested.