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Timothy K. Broschat and Thomas J. Weissling

The greenhouse orthezia (Orthezia insignis) is a serious and widespread pest of cultivated lantanas (Lantana sp.) in warmer regions of the world. Forty species and cultivars of lantanas were screened for their relative susceptibility to this insect pest. Results showed that two Florida native lantanas, pineland lantana (L. depressa) and buttonsage (L. involucrata), were highly susceptible to infestation, with trailing lantana (L. montevidensis) and its cultivars and hybrids being somewhat less susceptible. Shrub lantana (L. camara) and its cultivars and hybrids were the least susceptible to greenhouse orthezia infestation, but some of these varieties are rather unattractive as landscape ornamentals and can become serious weeds.

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Zongyu Li, R. Karina Gallardo, Wendy Hoashi-Erhardt, Vicki A. McCracken, Chengyan Yue and Lisa Wasko DeVetter

marketing channels used for strawberry, and general grower and operation characteristics. We presented five clusters of plant and fruit traits (fruit quality, disease resistance/tolerance, insect pest resistance/tolerance, plant stress tolerance, and other

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Wenbin Li, Kelly A. Zarka, David S. Douches, Joseph J. Coombs, Walter L. Pett and Edward J. Grafius

The codon-modified cryV-Bt gene (cryV-Bt) from Bacillus thuringiensis subsp. kurstaki Berliner, which is specifically toxic to Lepidoptera and Coleoptera insects, and a potato virus Yo coat protein gene (PVYocp), in which the aphid transmission site was inactivated, were cotransformed into potato (Solanum tuberosum L.) `Spunta' via Agrobacterium tumefaciens Conn. We demonstrated the integration and expression of both genes by molecular analysis and bioassays. All cryV-Bt/PVYocp-transgenic lines were more resistant to potato tuber moth (Phthorimaea operculella Zeller) and PVYo infection than nontransgenic `Spunta'. Four cryV-Bt/PVYocp transgenic lines were equal in potato tuber moth mortality to a cryV-Bt transgenic line, but of these four only two lines were equivalent in PVYo titer levels to a PVYocp-transgenic line. We identified two transgenic lines, 6a-3 and 6a-5, which showed greater resistance to potato tuber moth and PVYo than the other cryV-Bt/PVYocp transgenic lines. This study indicated that multiple genes, conferring insect pest resistance and virus resistance, could be engineered into and expressed simultaneously in a potato cultivar.

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Patricia Yolanda Zapata-Castillo, Adriana-Canto Flick, Guadalupe López-Puc, Anabel Solís-Ruiz, Felipe Barahona-Pérez, Nancy Santana-Buzzy and Lourdes Iglesias-Andreu

morphogenesis, genetic improvement of pepper cultivars for disease and insect pest resistance, among other characters, can be efficiently achieved through genetic engineering. To date, difficulties in regeneration (shoot elongation from induced adventitious

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R. Karina Gallardo, Parichat Klingthong, Qi Zhang, James Polashock, Amaya Atucha, Juan Zalapa, Cesar Rodriguez-Saona, Nicholi Vorsa and Massimo Iorizzo

nonbreeder researchers. Five trait clusters were derived from this list, including 1) fruit quality, 2) disease resistance, 3) arthropod pest resistance (note that the survey used “insect pest resistance” to refer to arthropod pest resistance, which we use

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R. Karina Gallardo, Qi Zhang, Michael Dossett, James J. Polashock, Cesar Rodriguez-Saona, Nicholi Vorsa, Patrick P. Edger, Hamid Ashrafi, Ebrahiem Babiker, Chad E. Finn and Massimo Iorizzo

, including 1) fruit quality, 2) disease resistance, 3) arthropod pest resistance (note that the survey used “insect pest resistance” to refer to arthropod pest resistance, which we use hereafter, because some pests are not true insects), 4) plant stress