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Simone da Costa Mello, Jéssika Angelotti-Mendonça, Lucas Baiochi Riboldi, Luigi Tancredi Campo Dall’Orto, and Eduardo Suguino

1. Effect of indole-3-butyric acid (IBA) concentration (0, 30, 60, and 90 mg·L −1 ) and cuttings type (softwood and semihardwood) on cutting survival (CS), rooting, number of roots (NOR), dry weight of roots (DWR), and root length (RL) of ‘Yabukita

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Karla Gabrielle Dutra Pinto, Sônia Maria Figueiredo Albertino, Bruna Nogueira Leite, Daniel Oscar Pereira Soares, Francisco Martins de Castro, Laís Alves da Gama, Débora Clivati, and André Luiz Atroch

exogenous auxin for the rooting of stem cuttings ( Ramos et al., 2003 ). The rooting method used for guarana seedling production suggests 70% shading, intermittent overhead mist, and the application of 2000 ppm indole-3-butyric acid (IBA); however, this

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Benjamin E. Deloso, Anders J. Lindström, Frank A. Camacho, and Thomas E. Marler

, and 100% for the 3, 8, or 30 mg·g −1 plants. Table 1. The response of Zamia furfuracea and Zamia integrifolia stem cuttings to indole-3-butyric acid concentrations of 0, 3, 8, 16, or 30 mg·g −1 . N = 25. The Z. integrifolia IBA study lasted 356

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Adigun McLeod, Kelly Vining, Tyler Hoskins, and Ryan Contreras

. A representation of the two-factor indole-3-butyric acid (IBA) formulation × IBA concentration interaction. Root and shoot quality are presented on a 0–4 relative scale for ‘I3’ hemp stem cuttings, with 4 being the best quality. A rating of 0 for

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Trent Y. Hata, Arnold H. Hara, Mike A. Nagao, and Benjamin K.S. Hu

Frangipani (Plumeria hybrid `Donald Angus') cuttings immersed in hot water (49C for 10 min) followed by 0.8% indole-3-butyric acid (IBA) basal treatment (hot water + IBA) had greater root length and weight compared to the nontreated control, hot water, or IBA treatment alone. Greater percentage of rooting and number of roots per cutting were observed for hot-water-treated + IBA-treated cuttings compared to the non-treated control and hot-water treatment alone. In a second study, Dracaena fragrans (L.) Ker-Gawl. `Massangeana', D. deremensis Engl. `Warneckii', D. deremensis Engl. `Janet Craig', D. marginata Lam., and cape jasmine (Gardenia jasminoides Ellis) cuttings displayed results similar to those observed with Plumeria cuttings. In addition to enhancing rooting, hot water + IBA also stimulated the number of shoots per cutting on anthurium (Anthurium andraeanum Andre `Marian Seefurth'), croton [Codiaeum variegatum (L.) Blume var. pictum (Lodd.) Mull. Arg.], D. marginata, D. fragrans, Plumeria, and ti (Cordyline terminalis `Ti') cuttings.

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Darrell Sparks and J. W. Chapman


The percentage of rooting and survival of 1-year old non-juvenile pecan branches air-layered 50 days after bud break and left on the tree for about 5 1/2 months increased as indole-3-butyric acid (IBA) was increased from 0 to 3%. After 1 season’s growth, maximum survival occurred with air-layers that had been treated with 3% IBA. Shoot growth of the air-layers was relatively short.

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James F. Harbage, Dennis P. Stimart, and Carol Auer

The influence of root initiation medium pH on root formation was investigated in relation to uptake and metabolism of applied IBA in microcuttings of Malus ×domestica Borkh. `Gala' and `Triple Red Delicious'. Root formation and uptake of H 3-IBA were related inversely to root initiation medium pH. Maximum root count (10.3 roots) and IBA uptake were observed at pH 4.0. Regardless of pH, overall root count of `Gala' was higher (13.5 roots) than `Triple Red Delicious' (4 roots). Uptake of IBA was highest at pH 4.0 for `Gala' (1.7% uptake) and at pH 4 and 5 for `Triple Red Delicious' (0.75% uptake). Metabolism of IBA was the same regardless of root initiation medium pH or cultivar examined. One-half of the IBA taken up was converted to a compound that coeluted with IBAsp during high-performance liquid chromatography. Apparently, pH regulates root formation by affecting IBA uptake but not metabolism. The level of auxin in tissue appeared unrelated to root formation between genotypes. Chemical names used: 1H-indole-3-butyric acid (IBA); 5-H 3-indole-3-butyric acid (H 3-IBA); indole-3-butrylaspartic acid (IBAsp).

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Jyotsna Sharma, Gary W. Knox, and Maria Lucia Ishida

Certain cultivars of magnolia are desirable in landscapes for their uncommon yellow flowers. While cultivars derived from Magnolia acuminata L. (cucumbertree magnolia) are difficult to propagate by stem cuttings, some with mixed parentage appear easier to propagate in this manner. We propagated six yellow-flowered cultivars vegetatively by applying 0, 8, 16, or 30 g·kg–1 (0, 8,000, 16,000, or 30,000 ppm) indole-3-butyric acid (IBA) in talc to bases of terminal stem cuttings collected 5, 7, 9, or 11 weeks after budbreak. Mean rooting percentage over all cultivars increased from 12% (in the absence of IBA) to 34% (after application of 30 g·kg–1 IBA). Rooting percentage and basal stem diameter of a cutting did not seem related. For each collection date, more cuttings of `Ivory Chalice' and `Yellow Lantern' developed roots than the other cultivars. More roots (mean = 5) developed on cuttings of `Yellow Lantern' collected 5 weeks after budbreak or when treated with 30 g·kg–1 IBA than the other cultivars. `Butterflies' largely remained unresponsive, whereas rooting of `Golden Sun,' `Hot Flash,' and `Maxine Merrill' collected 5 weeks after budbreak was 31%, 22%, and 28%, respectively. When data were analyzed separately for selected cultivars, 63% rooting was observed among cuttings of `Ivory Chalice' collected 7 weeks after budbreak. Rooting percentage was higher (22%) among cuttings of `Hot Flash' collected 5 or 7 weeks after budbreak in comparison to later collection dates, but harvest date did not influence rooting of `Yellow Lantern,' which ranged from 44% to 59%. Collection of stem cuttings early in the growing season (5 weeks after budbreak) was beneficial (31% rooting) for inducing rooting among cuttings of `Golden Sun.' We conclude that `Ivory Chalice' and `Yellow Lantern' are promising choices for growers interested in clonal propagation of yellow-flowered cultivars of magnolia. To maximize rooting among these cultivars, terminal cuttings should be collected within 5 to 11 weeks after budbreak and treated with 16 or 30 g·kg–1 IBA in talc. Early collection dates (5 to 7 weeks after budbreak) improved rooting among cuttings of other cultivars but these, particularly `Butterflies,' remain variably recalcitrant and merit further study.

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James F. Harbage and Dennis P. Stimart

Involvement of pH and IBA on adventitious root initiation was investigated with Malus domestica Borkh. microcuttings. The pH of unbuffered root initiation medium (RIM) increased from 5.6 to 7 within 2 days. Buffering with 2[N-morpholino] ethanesulfonic acid (MES) adjusted to specific pHs with potassium hydroxide prevented pH changes and resulted in a 2-fold higher root count at pH 5.5 compared to pH 7 or unbuffered medium. As pH decreased, lower concentrations of IBA were required to increase root counts. Colorimetric measurement of IBA in buffered RIM showed greater IBA loss and higher root count were associated with lower pH levels in all cultivars. This suggests that IBA loss from RIM depends on medium pH, which affects root count. Root count differences between easy-to-root through difficult-to-root cultivars were not consistent with amount of IBA loss from RIM. Cultivar differences in root count could not be explained solely by IBA loss from RIM.

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Benjamin D. Taylor and Benjamin K. Hoover

cuttings treated with 0, 1000, or 3000 ppm (μg·g −1 ) indole-3-butyric acid applied via a talc dip or a foliar spray ( n = 7). The leftmost column contains the four root response metrics, each with the factors analyzed in the model: block, auxin rate