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Boyang R. Cao and Chih-Cheng T. Chao

Polymorphisms of 21 date cultivars (Phoenix dactylifera L.) in California were determined by amplified fragment length polymorphism (AFLP) analysis with near infrared fluorescence labeled primers. Four primer sets were used to detect polymorphisms. Based on the UPGMA-cluster analysis of 328 polymorphic bands, the majority of the cultivars was separated into two major groups. Cultivars Abada, Amir Hajj, Ashrasi, Bentamoda, Boyer No. 11, Deglet Beida, Horra, Javis No. 1, Khadrawy, and Thoory belonged to group I. Cultivars Badrayah, Dayri, Halawy, Haziz, Khir, Medjool, Sayer, and Zahidi belonged to group II. Cultivars Barhee and Deglet Noor were further separated from groups I and II. `Hayany' was distinct from all other date cultivars tested. These results demonstrated that AFLP markers could efficiently identify individual date cultivars. The information will be useful for future date germplasm collection and facilitated selection of diverse parents for cross hybridization in a breeding program.

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Kristin R. Campbell, Sandra B. Wilson, P. Christopher Wilson and Zhenli He

students through the respective e-learning site (which is no longer available after the course ends). This project developed a series of innovative plant identification tools for open-sourced, asynchronous applications. These materials can be used by

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Àngel Fernández i Martí, José M. Alonso, María T. Espiau, María J. Rubio-Cabetas and Rafel Socias i Company

-genotype identification ( Kodad et al., 2008 ). Genetic diversity has been traditionally assessed by phenotypic observations, mainly based in the International Board for Plant Genetic Resources [IBPGR (now Bioversity International)] descriptors ( Gülcan

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Kim D. Bowman

species, including wood, plastic, metal, and paint, but all have faults that limit their use and useful life. Implantation of radiofrequency identification device (RFID) microchips is widely used for labeling of pets and livestock and in many types of work

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Hafid Achtak, Ahmed Oukabli, Mohammed Ater, Sylvain Santoni, Finn Kjellberg and Bouchaib Khadari

also been used in the agrofood industry for identification of virgin olive oil ( Pasqualone et al., 2004 ). In Morocco, fig germplasm is maintained in an ex situ collection at INRA Meknes. It was established from prospecting Moroccan areas, especially

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Andrea Luvisi, Alessandra Panattoni, Roberto Bandinelli, Enrico Rinaldelli, Mario Pagano, Barbara Gini, Giorgio Manzoni and Enrico Triolo

RFID technology has been introduced efficiently in animal identification systems ( Artmann, 1999 ; Jansen and Eradus, 1999 ). RFID applications in the plant sector mainly involve food traceability, logistics, or harvest, with the microchip (TAG

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Warren F. Lamboy and Christopher G. Alpha

The USDA-ARS Vitis genetic resources collections in Geneva, N.Y., and Davis, Calif., contain ≈3600 accessions of >35 species. Accurate and unambiguous identification of these grapes is essential for efficient and effective use of this germplasm. Previous workers have successfully used polymerase chain reaction (PCR)-generated SSRs to fingerprint cultivars of the wine and table grape species, V. vinifera. Building on this work, we conducted a test of five previously characterized SSR loci on 110 accessions of 25 grape taxa (21 Vitis species and 4 hybrids) to determine if they would satisfy our need for identifying cultivars within the USDA-ARS grape collections. Scorable SSR fragments were produced with all 550 primer-accession combinations, with no null loci observed. The loci were highly polymorphic, with 16 to 38 different alleles found at a locus. Heterozygosity values ranged from 0.464 to 0.818, while gene diversity values ranged from 0.875 to 0.955. Discrimination power at a locus varied from a low of 0.947 to a high of 0.987. Combined discrimination power of all loci was effectively 1.000, with 2 chances in 100,000,000 that two sexually, independently derived grape accessions would not be distinguishable using this set of five SSR loci. Two plants in the study that had previously been classified as belonging to different grape species were shown to have identical SSR fingerprints, showing that they almost certainly possessed the same genotype. Because SSR markers are codominant and highly polymorphic and SSR loci are generally conserved across a range of related species, we strongly recommend SSRs for fingerprinting not only grape, but other clonal genetic resources collections as well.

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Rhiannon L. Wilson and William A. Hoch

Dirr, M.A. 1998 Manual of woody landscape plants: Their identification, ornamental characteristics, culture, propagation and uses 5th Ed Stipes Publishing Champaign, IL Dirr, M.A. Heuser, C.W. 1987 The

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Yushu Li, Zongda Xu, Weiru Yang, Tangren Cheng, Jia Wang and Qixiang Zhang

.1 ( Tamura et al., 2011 ) and the neighbor-joining method with the following parameters: Poisson correction, complete deletion, and bootstrap (1000 replicates). Paraffin sectioning and identification of floral bud differentiation stage. To analyze the

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Zuguo Cai, Wenfang Zeng, Liang Niu, Zhenhua Lu, Guochao Cui, Yunqin Zhu, Lei Pan, Yifeng Ding and Zhiqiang Wang

., 2011 ; Moing et al., 2003 ). Increasing numbers of related species are being used to develop new peach cultivars. Efficient and accurate species identification and hybrid analysis are necessary in peach breeding and germplasm management. A range of