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Young Rog Yeoung and Dale O. Wilson

Pregermination techniques of osmotic priming and hydropriming have been used to enhance seed performance on planting Osmotic priming and hydropriming method were compared on the basis of germination performance O2, N2 and air were supplied to 500 ml vessels containing seeds with distilled water or -1.31 MPa PEG solution for 10 days On removing seeds from vessels, seeds were dried back to original water content. There were no differences in total germination between osmotic priming and hydropriming treatments t50 was reduced dramatically from 112 to 32 hours, using hydropriming with air and N2supply for 1 day, compared to 70 hours of osmotic priming. Solute leakage from O2supply of both methods was higher than air or N treatment, Indicating the loss of membrane integrity Hydropriming with O2induced radicle emergence and loss of desiccation tolerance around 28 hours after treatment LEA protein levels were not changed in both treatments except for hydropriming with O2. The timing of desiccation tolerance loss was correlated with that of degradation of LEA protein. O2supply caused the adverse effects on seed performance from both methods 1 day after treatment

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Sunitha Gurusinghe, Ann L.T. Powell, and Kent J. Bradford

While seed priming (hydration in water or osmotic solutions followed by drying) enhances seed germination performance, the longevity of primed seeds in storage often is reduced. Postpriming treatments including a reduction in seed water content followed by incubation at 37 or 40 °C for 2 to 4 h can substantially restore potential longevity in tomato (Lycopersicon esculentum Mill.) seeds. These conditions might induce heat-shock proteins (hsp) that could be involved in the extension of seed longevity. The abundance of BiP (78 kD Binding Protein), hsp70 and class I small hsp in primed seeds subjected to postpriming treatments was examined to assess this possibility. BiP mRNA and protein amounts increased during postpriming heat treatments that extended longevity of tomato seeds. Treatment of primed seeds with the calcium ionophore calcimycin (A21387) enhanced BiP protein accumulation in the absence of heat treatment and also extended potential seed longevity. Changes in the abundance of hsp70 and class I small hsps were not consistently associated with potential seed longevity. Thus, enhanced BiP expression may contribute to the improved longevity of primed seeds following postpriming treatments.

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Maria A. Macias-Leon and Daniel I. Leskovar

southwest of the United States and Mexico. Five grams of dry seeds of each cultivar were soaked in 30 mL of hormonal solutions for 10 h as described in Table 1 ( Kakei et al., 2015 ); in addition, water (hydro-priming) and dried seeds were included as

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Qi Zhang, Kevin Rue, and Jeanna Mueller

water (i.e., hydropriming) or other solutions. The embryo is pre-enlarged and the germination rate is enhanced ( Austin et al., 1969 ; Gray and Steckel, 1977 ). Research shows that seed priming enhances germination in many crops, especially under

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Kun Jia, Michelle DaCosta, and J. Scott Ebdon

(inhibited) by drying of the seeds. In a recent review of priming agents in agricultural crops ( Jisha et al. (2013) , numerous primers, including water (hydropriming), hormonal, osmotic, matric, and redox primers, have been shown to enhance final germination

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Clíssia Barboza da Silva, Julio Marcos-Filho, Pablo Jourdan, and Mark A. Bennett

. Priming with water alone (hydropriming) can improve the efficiency of germination, but imbibition in the presence of other various compounds (nutrients, salicylic acid, growth regulators, etc.) has shown additional benefits ( Parera and Cantliffe, 1994