Organic production is a fast-growing sector of agriculture in need of variety evaluations under their unique production systems. This study evaluated 16 watermelon (Citrullus lanatus) varieties for their performance characteristics under organic production practices. Plants were grown on plastic mulch-covered beds on land that had been certified organic in accordance with the U.S. Department of Agriculture National Organic Program. Six of the entries were F1 hybrids; the remaining entries were open-pollinated (OP) varieties. Of the 10 OP varieties, three were considered heirloom varieties, including Cream of Saskatchewan, Georgia Rattlesnake, and Moon & Stars. ‘Georgia Rattlesnake’ was the highest yielding variety and had the greatest average fruit weight. Along with ‘Georgia Rattlesnake’, ‘Nunhems 800’, ‘Nunhems 860’, ‘Orangeglo’, and ‘SSX 8585’ were included in the top five yielding varieties. The top five yielding varieties had fruit size that averaged more than 20 lb. Fruit size correlated with rind thickness, with lighter fruit having thinner rind (Pearson’s correlation, r = 0.779), which is not unexpected. ‘Sangria’ had the greatest average soluble solids content at 11.2%, which was greater than all entries with soluble solids less than 10%.