Search Results

You are looking at 1 - 10 of 59 items for :

  • "heat injury" x
  • Refine by Access: All x
Clear All
Open access

Dewayne L. Ingram and David Buchanan

Abstract

Electrolyte leakage was used to measure direct heat injury to roots of Illicium anisatum L., Ilex cornuta L. cv. Burfordii and Juniperus chinensis L. cv. Parsonii. A sigmoidal relationship was found between percent electrolyte leakage and temperature treatment. About 50% electrolyte leakage was realized from a 20 minute exposure of roots to 50.5 ± 0.5°, 48.5 ± 0.5° and 46.5 ± 0.5°C for I. anisatum, J. chinensis, and I. cornuta, respectively.

Free access

Jun Song, Lihua Fan, Charles F. Forney, and Michael A. Jordan

Volatile emissions and chlorophyll fluorescence were investigated as potential signals of heat injury for apple [Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf.] fruit. `McIntosh', `Cortland', `Jonagold', and `Northern Spy' apples were exposed to 46 °C for 0, 4, 8, or 12 hours (heat treatments). Following treatments, fruit were kept at 20 °C and evaluated after 1, 2, 4, or 7 days. Heat treatments induced volatile production including ethanol and ethyl acetate. The 8 and 12 hours heat treatments increased ethanol and ethyl acetate production in all four cultivars by as much as 170- and 11-fold, respectively, 1 day after treatments. Heat treatments also reduced ethylene production and chlorophyll fluorescence. Heat for 12 hours caused serious flesh browning. Among the cultivars investigated, `Northern Spy' and `McIntosh' were most susceptible to heat stress based on the degree of flesh browning. Correlation coefficients of heat stress induced ethanol emission and chlorophyll fluorescence with flesh browning were 0.82 and -0.66, respectively. The nondestructive measurements of ethanol emission and chlorophyll fluorescence have potential to identify stressed fruit with reduced quality or compromised storage life.

Free access

S.J.R. Underhill and C. Critchley

Mature lychee (Litchi chinensis Sonn.) fruit were heat-treated at 60C for 10 min to study heat-induced pericarp browning. Polyphenol oxidase (EC 1.10.3.2) activity of the pericarp increased immediately, corresponding with rapid anthocyanin degradation, Tissue browning was observed 2 min after heating, with pigmentation distributed uniformly throughout the pericarp. The distribution of brown pigments was different than the highly localized browning observed under ambient desiccation. Although both ambient and heat-induced pericarp browning are visually similar, the anatomical distribution of brown pigmentation is quite distinct. The distribution of brown pigmentation was not consistent with anthocyanin localization. Following ambient desiccation, the mesocarp became colorless even though this represented the greatest concentration of pigment. Browning caused by heating may result from nonselective degradation of a range of compounds, including anthocyanin.

Free access

John M. Ruter

Membrane thermostability of `Needlepoint' Chinese holly (Ilex cornuta Lindl. & Paxt.), `Albo-marginata' English holly (Ilex aquifolium L.), and `Nellie R. Stevens', an Ilex aquifolium × Ilex cornuta hybrid, was determined by measuring electrolyte leakage in excised leaves and roots. The critical midpoint heat-killing temperature (T,) after a 30-min exposure was 54.4 ± 0.4C for `Nellie R. Stevens' leaves and was ≈ lC higher than that for Chinese (52.9 ± 0.3C) or English holly (52.9 ± 0.4C). The Tm for English holly roots (53.9 ±_ 1.5C) was higher than that for either `Nellie R. Stevens' (51.7 ± 0.3C) or Chinese holly (50.1 ± 0.3C). The results of this study suggest that English holly and `Nellie R. Stevens' leaves and roots can withstand direct heat injury equal to or greater than that of Chinese holly.

Free access

Keryl K. Jacobi and Don Gowanlock

Mature green `Kensington' mango fruit were submerged in hot water at 46C until the fruit center reached 45C and then held for 30 minutes. The fruit were allowed to ripen for 7 to 10 days after the hot water treatment, and then damaged areas of skin and mesocarp tissue were prepared for observation by scanning and transmission electron microscopy. Heating-related injuries included rupturing the patterned cuticle and exocarp and exposing the underlying cells and hollow cavities (which varied in size and shape) randomly distributed within the mesocarp beneath the skin. Starch deposits still were present in the mesocarp parenchyma cells. The cell walls of damaged mesocarp parenchyma cells were convoluted and thickened in places. The injury suggested disruption of enzymes involved in carbohydrate metabolism.

Free access

Yali He, Xiaozhong Liu, and Bingru Huang

Various physiological processes may deteriorate in response to increasing temperatures, contributing to the decline in turf quality for cool-season turfgrasses during heat stress. This study was performed to investigate metabolic changes (membrane lipid peroxidation, total protein content, amino acid content, and protease activity) associated with turf quality decline for creeping bentgrass (Agrostis stolonifera Huds.) in response to gradually increasing temperatures for a short duration and prolonged exposure to lethally high temperature. Plants were subjected to increasing temperatures of 20, 25, 30, 35, and 40 °C for 7 days at each level of temperature [gradual heat stress (GHS)] or exposed to high temperature of 40 °C for 28 days [prolonged heat stress (PHS)] in growth chambers. During the GHS treatment, significant decline in turf quality occurred when plants were exposed to 30 °C for 7 days; simultaneously, malondialdehyde (MDA) content increased and total protein content in shoots decreased significantly compared to those at 20 °C. Protease activity increased at 25 °C and then decreased as temperature was elevated from 30 to 40 °C during the GHS treatment. Amino acid content decreased under GHS, beginning at 25 °C. Under the PHS treatment, turf quality declined and MDA content increased significantly, beginning at 14 days of PHS, while total protein content decreased at 7 days of PHS. Protease activity and amino acid content increased at 7 days of PHS, and then declined with longer stress duration. Our results indicated that protease activity, and amino acid and total protein content were more responsive to GHS or PHS than that of lipid peroxidation and turf quality. Changes in metabolic parameters of protease activity, amino acid and total protein content, and lipid peroxidation may contribute to leaf senescence and poor turf performance under severe or prolonged heat stress conditions for creeping bentgrass.

Open access

Melissa M. Gibbs, Thomas M. Blessington, James A. Price, and Yin-Tung Wang

Abstract

Pot-grown ‘Angie Physic’ hibiscus (Hibiscus rosa-sinensis L.) plants at the tight bud and blooming stages were stored in darkness for 3, 6, or 9 days at 4.5, 10.0, 15.5, 21.0, 26.5, or 32.0C, and then placed in a greenhouse for 21 days. Plants showed the least amount of damage at 10.0 or 15.5C or when stored for 3 days. Plants stored at 10.0 or 15.5C had delayed flowering, larger and more flowers, less flower bud and leaf abscission, and a higher plant quality. Storage for 6 or 9 days resulted in plants with smaller and fewer flowers, greater bud and leaf abscission, less fresh weight, and a lower quality.

Open access

Makoto Inaba and Kazuo Chachin

Abstract

Mature green (MG) tomatoes (Lycopersicon esculentum Mill. cv. Kyoryoku toko) were stored at 40°C for 1 to 3 days, then held at 25° for 8 days or stored at 35° for 1 to 6 days, then held at either 25° or 30° for an additional 12 days. During storage, respiration rate, C2H4 production, and color development were measured. The effect of temperature stress at 40° could be reversed if the fruit was exposed for < 1 day and then immediately cooled to 25°. At 35°, fruit ripening was suppressed but could have been reversed if exposure at 35° was < 3 days before the fruit was cooled to 25°. Holding at 30° did not completely reverse the suppression caused by 3 days at 35°C.

Open access

Werner J. Lipton

Abstract

Near ultraviolet radiation appears to be largely responsible for solar injury (SI) and vein tract browning (VTB) of cantaloupes (Cumis melo L., Reticulatus group, cv. PMR 45) grown under field conditions in the San Joaquin Valley of California. Incidence and severity of SI were substantially reduced when near UV flux impinging on the fruits at solar noon was reduced to 21 Wm-2 or less and especially when wavelengths below 320 nm were excluded by the use of plastic UV filters. VTB was reduced when UV flux was 12 Wm-2 or less or when wavelengths below 320 nm were excluded. In cantaloupes, near UV appears to directly induce SI but to indirectly induce VTB, a postharvest disorder, by accelerating aging of surface tissues.

Free access

Lihua Fan, Jun Song, Charles F. Forney, and Michael A. Jordan

Ethanol concentration and chlorophyll fluorescence (CF) were measured as signs of heat stress in apple fruit [Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf.]. `McIntosh', `Cortland', `Jonagold', and `Northern Spy' apples were placed in trays and exposed to 46 °C for 0, 4, 8, or 12 hours. Following treatments, fruit were stored in air at 0 °C and evaluated after 0, 1, 2, or 3 months. Ethanol and ethylene production, CF, peel and flesh browning, firmness, skin color, soluble solids, and titratable acidity were measured. Increases in ethanol were apparent immediately following 12-hour heat treatments as well as after 3 months. After 3 months, ethanol concentrations were 16-, 52-, 6-, and 60-fold higher in `McIntosh', `Cortland', `Jonagold', and `Northern Spy' apples than in controls, respectively. The concentrations of ethanol accumulated reflected the degree of heat-induced fruit injury. Heat treatments reduced ethylene production relative to control values. After 3 months of storage ethylene production of fruit exposed to 46 °C for 12 h was <0.48 μmol·kg-1·h-1 compared to >4.3 μmol·kg-1·h-1 for controls. Heat treatments also reduced CF which was expressed as Fv/Fm, where Fv is the difference between the maximal and the minimal fluorescence (Fm - Fo), and Fm is the maximal fluorescence. After 3 months storage at 0 °C, Fv/Fm was ≈0.2 in fruit held at 46 °C for 12 hours compared with 0.5-0.6 for control fruit. Exposure to 46 °C for 12 hours caused severe peel and flesh browning in all cultivars. Severity of peel and flesh browning increased with increasing duration of heat treatment and subsequent storage at 0 °C. `Northern Spy' apple fruit were most susceptible to heat stress based on the degree of flesh browning. Heat treatments of 8 and 12 hours reduced firmness of `McIntosh', `Cortland', and `Northern Spy', but not `Jonagold' apples. Hue angle of the green side of fruit was also reduced in `Cortland', Jonagold' and `Northern Spy' apples receiving the 8- and 12-hour treatments. Heat treatments caused a decrease in fruit tiratable acidity, but had no effect on soluble solids content. The increase in ethanol production and decrease in CF correlated with heat-induced injury, and were apparent before browning was visually apparent. Ethanol and CF have the potential to be used to nondestructively predict the severity of injury that develops during storage.