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Geoffrey Meru and Cecilia E. McGregor

.E. 2011 A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species PLoS One 6 e19379 Everts, K.L. Himmelstein, J.C. 2015 Fusarium wilt of watermelon: Towards sustainable management of a re-emerging plant disease Crop Prot. 73 93 99

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Lav K. Yadav, Edward V. McAssey and H. Dayton Wilde

more variation among the individuals than between the three population clusters (Supplemental Table 2), in agreement with previous results. Conclusions A genotyping-by-sequencing approach was taken to characterize the genetic structure and diversity of

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Jessica Chitwood, Ainong Shi, Beiquan Mou, Michael Evans, John Clark, Dennis Motes, Pengyin Chen and David Hensley

SNP genetic maps would be a valuable resource for spinach breeding efforts. Genotyping by sequencing is one of the next-generation sequencing platforms that uses a simple, highly multiplexed system for constructing reduced representation libraries. It

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Amnon Levi, Alvin M. Simmons, Laura Massey, John Coffey, W. Patrick Wechter, Robert L. Jarret, Yaakov Tadmor, Padma Nimmakayala and Umesh K. Reddy

Fusarium oxysporum race 1 resistance identified in genetic populations derived from closely related watermelon lines using selective genotyping and genotyping-by-sequencing for SNP discovery Theor. Appl. Genet. 127 2105 2115 Levi, A. Thomas, C.E. Keinath

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R. Karina Gallardo, Parichat Klingthong, Qi Zhang, James Polashock, Amaya Atucha, Juan Zalapa, Cesar Rodriguez-Saona, Nicholi Vorsa and Massimo Iorizzo

Covarrubias-Pazaran, G. Diaz-Garcia, L. Schlautman, B. Deutsch, J. Salazar, W. Hernandez-Ochoa, M. Grygleski, E. Steffan, S. Iorizzo, M. Polashock, J. Vorsa, N. Zalapa, J. 2016 Exploiting genotyping by sequencing to characterize the genomic structure of an

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Lidia Lozano, Ignasi Iglesias, Diego Micheletti, Michela Troggio, Satish Kumar, Richard K. Volz, Andrew C. Allan, David Chagné and Susan E. Gardiner

thousands of SNPs. This includes high-throughput SNP arrays ( Chagné et al., 2012a ; Verde et al., 2012 ) and more recently genotyping by sequencing as the throughput of next-generation sequencing techniques ( Elshire et al., 2011 ) has increased. The

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Gehendra Bhattarai and Shawn A. Mehlenbacher

libraries, the ratio was an intermediate 57% ( Gürcan et al., 2010 ). Although single nucleotide polymorphism (SNP) markers can be generated in large numbers by procedures including genotyping-by-sequencing [GBS ( Elshire et al., 2011 )] and double digest

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David H. Byrne, Patricia Klein, Muqing Yan, Ellen Young, Jeekin Lau, Kevin Ong, Madalyn Shires, Jennifer Olson, Mark Windham, Tom Evans and Danielle Novick

; Debener and Byrne, 2014 ). To accomplish this goal, several tasks need to be completed. 1) A rapid genotyping format needs to be developed. Thus far, the genotyping by sequencing protocol has been optimized for diploid rose and a consensus map with 3500

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Gerardo H. Nunez, Hilda Patricia Rodríguez-Armenta, Rebecca L. Darnell and James W. Olmstead

Interdisciplinary Center for Biotechnology Research at the University of Florida (Gainesville). Subsequently, sequencing data were processed with the TASSEL-UNEAK nonreference genotyping by sequencing SNP calling pipeline ( Lu et al., 2012 ) to produce a haplotype

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Michael Dossett, Jill M. Bushakra, Barbara Gilmore, Carol A. Koch, Chaim Kempler, Chad E. Finn and Nahla V. Bassil

loci (unpublished data). Recently, Bushakra et al. (2012) published the first linkage map of black raspberry, but they were only able to map 29 polymorphic markers in this species. Although it may be possible to use genotyping by sequencing to