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Eliezer E. Goldschmidt

interest in various aspects of the citron has arisen in recent years, concurrent with rapid development of molecular and genomic technologies, leading to a wealth of new information. The unique cultural, medical, and sacramental history of the citron has

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Schuyler S. Korban

Plant Functional Genomics. Dario Lester (ed.). 2005. Food Products Press. An imprint of the Howarth Press, Inc. 10 Alice Street, Binghampton, NY 13904-1580. p. 677. $89.95, softcover. www.haworthpress.com/store/product.asp?sku=5311 . ISBN: 1

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Thomas M. Davis, Kevin M. Folta, M. M. Shields, Robin L. Brese, Laura M. R. DiMeglio and Qian Zhang

The past year has brought substantial progress in the development of functional and structural genomic tools for strawberry. Sequencing of cDNA library clones from the cultivated strawberry Fragaria × ananassa and the diploid model species Fragaria vesca has provided more than 3000 new EST sequences. We have also constructed a large (∼40 kb) insert genomic (fosmid) library from F. vesca. About 33,000 fosmid clones have been picked and spotted onto hybridization filters. Filters have been successfully probed with three single copy gene probes, one gene family probe, and chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) probe sets. The combined cpDNA and mtDNA clone content of the library is about 11%. After correction for organelle insert content, the nuclear genome coverage of the library is about 6×. Complete sequencing of two fosmid clones identified 12 putative protein-encoding genes, four of which were organized in colinearity with the corresponding chromosomal region of Arabidopsis thaliana. We will sequence an additional 50 fosmid clones, and use the resulting sequence data as the basis for developing a novel marker technology, to be described. These genomic tools will provide a basis for connecting specific genes to specific traits in the octoploid, cultivated strawberry, paving the way for implementation of gene-based, marker assisted selection as a tool for strawberry breeders. Opportunity for cross-species comparisons of gene sequence and composition, as well as genome organization and linkage group structure, between Fragaria and other members of the economically important Rosaceae family has been significantly enhanced, thus expanding the relevance of the project results to peach, cherry, apple, rose, brambles, and many other Rosaceous species.

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Lisa J. Rowland, Anik L. Dhanaraj, Dhananjay Naik, Nadim Alkharouf, Ben Matthews and Rajeev Arora

several genes; thus, it is a quantitative trait ( Arora et al., 2000 ; Byrne et al., 1997 ; Howe et al., 2000 ; Jermstad et al., 2001 ). Considerable molecular and genomic evidence indicates that cold acclimation is a complex phenomenon involving

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Akira Kitajima, Atsu Yamasaki, Tsuyoshi Habu, Bannarat Preedasuttijit and Kojiro Hasegawa

detailed karyological analysis. Genomic in situ hybridization (GISH) has become a useful tool for the characterization of genomes and chromosomes in polyploids and somatic hybrids of herbaceous plants ( Raina and Rani, 2003 ), and GISH for identification

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Laura A. Levin, Kelly M. Langer, David G. Clark, Thomas A. Colquhoun, Jeri L. Callaway and Howard R. Moskowitz

the pilot study, and 161 completed experiments were acquired. The findings for this study can be found in the book Mind Genomics: The New Novum Organum Vol. 5b authored by Howard R. Moskowitz. Additionally, the pilot study allowed for an educated

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Iban Eduardo, Pere Arús, Antonio José Monforte, Javier Obando, Juan Pablo Fernández-Trujillo, Juan Antonio Martínez, Antonio Luís Alarcón, Jose María Álvarez and Esther van der Knaap

of QTLs using different population structures ( Tanksley, 1993 ) as well as the development of populations suitable for QTL analysis, such as introgression lines and near-isogenic lines (NILs). A NIL genomic library consists of a set of lines, each

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Rebecca Grumet*, Xiaofeng Wang, Mohamed Tawfik and Mitch McGrath

Genomics tools have become increasingly varied and valuable for crop improvement. While several species have been targeted for concerted genomic efforts, the majority of horticultural species have received limited attention. Despite the wide variety of important cucurbit crop species, the Cucurbitaceae family has had minimal effort. We have initiated projects to develop genomic tools for cucumber, Cucumis sativus L. Efforts include production of cDNA, yeast two-hybrid, and genomic libraries, and development of an EST database and website for cucumber genomics. Sequences of cucumber leaf ESTs so far indicate that the cDNA library is of high quality and has modest redundancy. Distribution of sequences, as nominally predicted from GeneBank BLAST analysis, indicates that expressed genes fall in the following general categories: photosynthesis (21%), DNA/RNA/protein synthesis (20%), metabolism (15%), signaling (5%), other (16%), and unknown proteins (23%). Cucumber sequence data have been deposited into GenBank and are available on the Michigan State Univ. website (http://genomics.msu.edu/cucumberdb). The yeast two-hybrid library has been successfully used to identify and characterize several genes based on interaction with key proteins of interest, including genes interacting with viral replicases and poly(A) binding protein. The genomic library has been verified to be of high quality and has been used to identify clones of interest.

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J.P. Prince, Y. Zhang, E.R. Radwanski and M.M. Kyle

The isolation of genomic DNA from Capsicum spp. (pepper) of the quality and quantity required for analyses based on nucleic acid hybridization and/or the polymerase chain reaction (PCR) has proven difficult. We report here a procedure that reliably yields sufficient quantities of good-quality pepper genomic DNA from a variety of Capsicum genotypes.

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Phillip N. Miklas, Valerie Stone, Carlos A. Urrea and James S. Beaver

A genetic linkage map of 170 RAPD markers mapped across 79 recombinant inbred lines (Dorado and XAN-176) reveal genomic regions that condition multiple disease resistance to fungal (Ashy Stem Blight—Macrophomina phaseolina), viral (bean golden mosaic virus—BGMV), and bacterial (common bacterial blight—Xanthomonas campestris pv. phaseoli) pathogens of common bean (Phaseolus vulgaris). A genomic site on linkage group US-1 had a major effect, explaining 18%, 34%, and 40% of the variation in phenotypic reaction to ashy stem blight, BGMV, and common bacterial blight disease, respectively. Adjacent to this region was a QTL conditioning 23% of the variation in reaction to another fungal pathogen, web blight (Thanatephorus cucumeris). A second genomic site on linkage group US-1 had minor affect on multiple resistance expression to the same fungal (15%), viral (15%), and bacterial (10%) pathogens. It is unknown whether these specific genomic regions represent a series of linked QTL affecting resistance to each disease separately or an individual locus with pleiotropic effect against all three pathogens.