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Jian-Feng Geng, Cheng-Song Zhu, Xiao-Wei Zhang, Yan Cheng, Yuan-Ming Zhang, and Xi-Lin Hou

increase the selection efficiency. Genetic linkage maps that are essential to the detection of QTL and other applications have been constructed for nearly all economically important plants. Although there are a number of genetic maps reported in B. rapa

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Liang L. Hong and Paul G. Thompson

Random amplified polymorphic DNA (RAPD) markers were analyzed in parents and progeny of four sweetpotato crosses. An average of 69 primers were tested and 23.5% produced well resolved polymorphic banding patterns. Each polymorphic primer had an average of 1.9 polymorphic bands resulting in 0.45 polymorphic fragments per primer tested. Phenotypic segregation ratios of 88% of polymorphic fragments fit those expected for hexaploid Mendelian inheritance. Numbers of linked polymorphic fragments and numbers of linkage groups were 13 and 5 for Cross A, 0 and 0 for Cross B, 23 and 3 for Cross C and 16 and 6 for Cross D. Those results indicated that RAPD markers have potential for a genetic linkage map in sweetpotato; however, many primers must be screened.

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Yayeh Zewdie, Michael J. Havey, James P. Prince, and Maria M. Jenderek*

Garlic has been propagated exclusively by asexual means since time immemorial. The recent discovery of male fertile garlic accessions allowed studies on genetics and garlic improvement. Single nucleotide polymorphism (SNP) and random amplified polymorphic DNA (RAPD) based genetic linkage map was developed for garlic using a segregating population derived from one plant of PI 540316. Progenies segregated for male fertility and other morphological characters. Distortion of segregation was observed for most of the markers. This was expected due to the segregation of recessive deleterious alleles present in the garlic genome. The map contained 23 loci distributed on five linkage groups. It covered 319 cM with the average of 18 cM between loci. Linkage with the male fertility (Mf) locus was established with SNP marker AOB155 (26.7 cM).

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M.R. Foolad, S. Arulsekar, and F. Bliss

A genetic linkage map of Prunus has been constructed using an interspecific F2 population generated from self-pollinating a single F1 plant of a cross between a dwarf peach selection (54P455) and an almond cultivar (Padre). This map consists of approximately 80 markers including 10 isozymes. 12 plum genomic, 19 almond genomic and 40 peach mesocarp specific cDNA clones. The backbone map will be used for identifying the genomic locations and characterization of genes governing important economic traits in the genus Prunus. Of particular interests are those genes associated with fruit ripening and mesocarp development in peach and almond.

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S. Rajapakse, L. E. Belthoff, R. E. Ballard, R. Scorza, W.V. Baird, R. Monet, and A. G. Abbott

We have constructed a genetic linkage map of peach consisting of RFLP, RAPD, and morphological markers, based on 78 F2 individuals derived from the self-fertilization of four F1 individuals originating from a cross between `New Jersey Pillar' and KV 77119. This progeny set was chosen because parental genotypes exhibit variation in canopy shape, fruit flesh color, and flower petal color, size, and number. The segregation of 81 markers comprised of RFLP, RAPD and morphological loci was analyzed. Low copy genomic and cDNA probes were used in the RFLP analysis. The current genetic map for the WV family contains 57 markers assigned to 9 linkage groups, which cover 520 cM of the peach nuclear genome. The average distance between two adjacent markers was 9 cM. Linkage was detected between Pillar (Pi) and double flowers (Dl). RFLP markers loosely linked to Pi, flesh color (Y), and white flower (W) loci were found. Twenty-four markers remain unassigned.

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A. Levi, C. E. Thomas, A. Davis, O.U.K. Reddy, Y. Xu, X. Zhang, S. King, A. Hernandez, G. Gusmini, and T. Wehner

Genetic linkage map is being constructed for watermelon based on a testcross population and an F2 population. The testcross map comprises 262 markers (RAPD, ISSR, AFLP, SSR and ASRP markers) and covers 1,350 cM. The map comprises 11 large linkage groups (50.7–155.2 cM), 5 medium-size linkage groups (37.5–46.2 cM), and 16 small linkage groups (4.2–31.4 cM). Most AFLP markers are clustered on two linkage regions, while all other marker types are randomly dispersed on the genome. Many of the markers in this study are skewed from the classical (Mendelian) segregation ratio of1:1 in the testcross or the 3:1 ratio in the F2 population. Although the skewed segregation, marker order appeared to be consistent in linkage groups of the testcross and F2 population. A cDNA library was constructed using RNA isolated from watermelon flesh 1 week (rapid cell division stage), 2 weeks (cell growth and storage deposition stage, 4 weeks (maturation stage), and 5 weeks (postmaturation stage) post pollination. Over 1,020 cDNA clones were sequenced, and were analyzed using the Basic Local Alignment Search Tool (BLAST). The sequenced cDNA clones were designated as expressed sequenced tag (EST) markers and will be used in mapping analysis of watermelon genome.

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Jack E. Staub, Zhanyong Sun, Sang-Min Chung, and Richard L. Lower

Genetic linkage mapping has historically been the basis for genomic investigation and the analysis of quantitative trait loci (QTL) ( Doerge, 2002 ). The construction of a detailed linkage map is, in fact, the initial step for the use of genetic

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Paul. G. Thompson, Liang L. Hong, Kittipat Ukoskit, and Zhiqiang Zhu

RAPD marker analyses were completed on parents and progeny of two sweetpotato [Ipomoea batatas (L.) Lam.] crosses to determine the feasibility of genetic linkage map construction. A total of 100 primers was tested and 96 produced amplified genomic DNA fragments. The average number of polymorphisms per primer was 0.69. A total of 134 polyphorphic markers was observed and 74 (60%) segregated 1 band present : 1 band absent as needed for use in genetic linkage mapping of polyploids. The 60% of RAPD markers that segregated 1:1 shows that genetic linkage mapping of the hexaploid sweetpotato by RAPD marker analysis is feasible. Linkage was determined for all markers that segregated 1:1 and five pairs of linked markers were found. These were the first linked molecular markers found in sweetpotato and they show that construction of a genetic linkage map is feasible. A genetic linkage map will be a valuable tool to assist in genetic improvements.

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Amnon Levi, Claude E. Thomas, Xingping Zhang, Tarek Joobeur, Ralph A. Dean, Todd C. Wehner, and Bruce R. Carle

A genetic linkage [randomly amplified polymorphic DNA (RAPD)-based] map was constructed for watermelon [Citrullus lanatus (Thunb.) Matsum and Nakai] using a BC1 population [PI 296341-fusarium wilt resistant × New Hampshire Midget (fusarium susceptible)] × `New Hampshire Midget'. The map contains 155 RAPD markers, and a 700-base pair sequenced characterized amplified region (SCAR) marker that corresponds to a fragment produced by the RAPD primer GTAGCACTCC. This marker was reported previously as linked (1.6 cM) to race 1 fusarium wilt resistance in watermelon. The markers segregated to 17 linkage groups. Of these, 10 groups included nine to 19 markers, and seven groups included two to four markers. The map covers a genetic linkage distance of 1295 cM. Nine of the 10 large linkage groups contained segments with low (or no) level of recombination (0 to 2.6 cM) among markers, indicating that the watermelon genome may contain large chromosomal regions that are deficient in recombination events. The map should be useful for identification of markers linked closely to genes that control fruit quality and fusarium wilt (races 1 and 2) resistance in watermelon.

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Yayeh Zewdie, Michael J. Havey, James P. Prince, and Maria M. Jenderek

Garlic (Allium sativum L.) has been cultivated by asexual propagation since time immemorial. The discovery of male-fertile garlic accessions has opened a venue for genetic studies and improvement through sexual recombination. An S1 family of 84 plants was generated from a single male-fertile heterozygous plant from the U.S. Dept. of Agriculture Plant Introduction 540316 and used to identify the first genetic linkages in garlic based on single nucleotide polymorphisms, simple sequence repeats, and randomly amplified polymorphic DNAs. Thirty-seven markers formed nine linkage groups covering 415 centimorgans (cM) with average distance of 15 cM between loci; other 16 loci remained unlinked. A male fertility locus was placed on the map. This first genetic map of garlic is a seminal step toward the genetic improvement of garlic and eventual marker-assisted breeding.