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Manuel Di Vecchi Staraz, Roberto Bandinelli, Maurizio Boselli, Patrice This, Jean-Michel Boursiquot, Valérie Laucou, Thierry Lacombe and Didier Varès

of ‘Sangiovese’, and 3) to extend the analysis to genetic structuring with the potential to distinguish cultivars of common ancestry and those related to ‘Sangiovese’ (M. Di Vecchi Staraz, unpublished data; Schaal et al., 1998 ). Materials and

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Jasmina Muminović, Andrea Merz, Albrecht E. Melchinger and Thomas Lübberstedt

Twelve amplified fragment length polymorphism (AFLP) primer combinations and 10 inter-simple sequence repeat (ISSR) primers were applied to estimate genetic diversity among 68 varieties of cultivated radish (Raphanus sativus L.). The material consisted of open-pollinated varieties, inbred lines, diploid and a few tetraploid hybrid varieties of garden radish [R. sativus var. sativus DC. convar. radicula (DC.) Alef.] and black radish [R. sativus var. niger (Mill.) Pers.]. Two accessions of uncultivated relatives of radish that as weeds cause serious contamination during the process of hybrid radish production were added to the analyses. Polymorphic fragments were scored for calculation of Jaccard's coefficient of genetic similarity (GS). Substantial level of genetic variability (average AFLP-based GS = 0.70; average ISSR-based GS = 0.61) was detected in the available germplasm of cultivated radish. Cluster analyses separated two weedy species from the cultivated germplasm. Within cultivated material, black radish and french breakfast radish types formed separate clusters. Based on AFLP data, a principal coordinate analysis (PCoA) and model-based approach revealed the genetic structure within cultivated radish germplasm and indicated the existence of divergent pools. Although the model-based approach did not separate black radish from french breakfast radish varieties, it offered a clear sub-division within garden radish germplasm. The results of this study may be relevant for hybrid radish breeding.

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Fuad Gasi, Kenan Kanlić, Belma Kalamujić Stroil, Naris Pojskić, Åsmund Asdal, Morten Rasmussen, Clive Kaiser and Mekjell Meland

cost of using an ever larger set of markers, in the analyses of big collections, is not always justified. Aside from accurately fingerprinting the collected accessions, SSR data can be used to investigate the underlying genetic structure and thus gain a

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Marissa Moses and Pathmanathan Umaharan

their unavailability at the time of this work. The objectives of this study were therefore to use RAPD-based markers to determine the genetic structure within accessions sampled and to estimate the diversity within the groups identified. Materials and

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Fuad Gasi, Silvio Simon, Naris Pojskic, Mirsad Kurtovic, Ivan Pejic, Mekjell Meland and Clive Kaiser

common genetic structure of the genotypes sampled from these two regions. Fst value calculated for all 108 analyzed accessions (international and traditional B&H reference cultivars included) was significant (0.019; P < 0.0001) but much lower than that

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Pan-Hui Huang, Wen-Bin Yu, Jun-Bo Yang, Hong Wang and Lu Lu

al., 1996 ). In this study, we developed new microsatellite markers for further estimating the genetic diversity and population genetic structure of P. rex . Twenty-two individuals of P. rex collected from five geographical locations in China

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C.R. Clement and R.M. Manshardt

The pejibaye (Bactris gasipaes Kunth) is being evaluated in Hawai'i for its fresh heart of palm, a gourmet vegetable. Seven half-sib progenies of the Putumayo land race were planted in a split-plot design, with densities (3333, 5000, 6666 plants/ha) as the main plots, progenies as the sub-plots, three replications, and nine plants/plot. Precocity was defined as “days from planting to harvest”; relative growth rate (RGR) and unit leaf rate (Ea) are possible causes of precocity and were estimated for the period from 6 months after planting to harvest. Density effects were never significant, suggesting that competition is not significant before harvest. Mean precocity ranged from 610 to 712 days; Va accounted for 14% of the phenotypic variance (Vp), with h2 = 0.57, similar to fruiting precocity in African oil palm (Elaeis guineensis Jacq.). Mean Ea ranged from 1.89 to 2.21 g/m2 per day, Va accounted for 8% of Vp, with h2 = 0.33 Mean RGR ranged from 0.0086 to 0.0102 d–1; Va accounted for 9% of Vp, with h2 = 0 35 Neither RGR (r = 0.20) nor Ea (r = 0.19) are significantly correlated with precocity. Heart, edible stem, and total edible product weights did not present significant progeny effects, probably because of the criterion used to determine harvest (height = 1.3 m). Precocity is easiest to work with and should give acceptable genetic gains.

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Yu Zong, Ping Sun, Xiaoyan Yue, Qingfeng Niu and Yuanwen Teng

clustering approach executed in STRUCTURE 2.3.4 ( Pritchard et al., 2000 ). This approach revealed genetic structure by assigning individuals or predefined groups to K clusters. For the current study, we chose the admixture model without using population or

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Jana Murovec

, N.E. (ed.). Genetic structure of populations. University Press of Hawaii, Honolulu, HI Paris, H.S. 1986 A proposed subspecific classification for Cucurbita pepo Phytologia 61 133 138 Paris, H.S. 2000 Segregation distortion in Cucurbita pepo , p

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Hai-fei Yan, Xue-jun Ge, Chi-ming Hu and Gang Hao

of polymorphic microsatellite loci from the genome of P. obconica to investigate the population genetic diversity and genetic structure, which is very important for studying its adaptation and evaluating its genetic resources. Total genomic DNA