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Xiangli Ma, Min Tang, Yufen Bi, and Junbo Yang

the two matrices. Genetic diversity ( H S ), the total genetic diversity ( H T ), and the genetic differentiation coefficients ( G ST and N ST ) were calculated using Permut version 2.0 software ( Pons and Petit, 1996 ), G ST and N ST were

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Yu Zong, Ping Sun, Xiaoyan Yue, Qingfeng Niu, and Yuanwen Teng

( Newton et al., 1999 ). Simple sequence repeats (SSRs) in nuclear genome, which are randomly distributed across eukaryotic genomes, have been widely used to detect genetic differentiation within species because of their high polymorphism levels and

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Fuad Gasi, Silvio Simon, Naris Pojskic, Mirsad Kurtovic, Ivan Pejic, Mekjell Meland, and Clive Kaiser

traditional B&H and international reference cultivars, whereas the groups of accessions from Sarajevo and eastern Bosnia overlapped. Because the genetic differentiation between these groups is negligible (nonsignificant values for Fst and P in AMOVA), this

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Tiantian Zhao, Wenxu Ma, Qinghua Ma, Zhen Yang, Lisong Liang, Guixi Wang, and Lujun Wang

), Shannon’s information index ( I ), observed heterozygosity ( Ho ), expected heterozygosity ( He ), polymorphism information content ( PIC ), coefficient of inbreeding ( F is ), genetic differentiation coefficient ( F st ), gene flow ( Nm ) and Nei

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S. Jorge, M.C. Pedroso, D.B. Neale, and G. Brown

Random amplified polymorphic DNA (RAPD) analysis was used to estimate genetic similarities between Portuguese Camelliasinensis (L.) O. Kuntze (tea plant) accessions and those obtained from the germplasm collections from the Tea Research Foundation of Kenya and from the National Research Institute of Vegetables, Ornamental Plants, and Tea of Japan. The accessions studied are taxonomically classified as C. sinensis, var. sinensis, var. assamica, or ssp. lasiocalyx. A set of 118 ten-base arbitrary primers was tested, of which 25 produced informative, reproducible, and polymorphic banding patterns. These primers were used to amplify DNA from 71 tea plant accessions and produced a total of 282 bands, of which 195 were polymorphic. The phenotypic frequencies were calculated using Shannon's Index and employed in estimating genetic diversity within tea plant populations. Our study demonstrates that tea plant populations, including the Portuguese tea plants, show considerable genetic variability. From the UPGMA cluster analysis based on a matrix using the Jaccard coefficient, it was possible to distinguish the Portuguese tea plants from the remaining accessions. The RAPD markers discriminated the three C. sinensis varieties. Moreover, within each variety cluster, subclusters formed according to geographic distribution. The RAPD analysis also separated the commercially cultivated tea plants from the Taiwanese wild tea plants. The present results show that RAPD analysis constitutes a good method to estimate genetic diversity within C. sinensis, and to differentiate C. sinensis accessions according to taxonomic variety and geographical distribution.

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Daniela Salvini, Silvia Fineschi, Roberta Pastorelli, Federico Sebastiani, and Giovanni G. Vendramin

Twenty populations of the species aggregate Rubus fruticosus were collected throughout European natural forests and analyzed by chloroplast microsatellites (SSR). Results showed high genetic diversity (h T = 0.73) and haplotipic richness (17 haplotypes were detected), and the presence of several unique alleles. The value of genetic differentiation between populations was low for unordered alleles (G ST = 0.29) and for ordered alleles (N ST = 0.30), revealing the absence of phylogeographic structure of the haplotypic diversity. This can be mainly ascribed to the mechanisms of seed dispersal, mostly mediated by animal ingestion, which are responsible for a efficient gene flow through seeds. Rubus L. species are characterized by the ability to colonizing disturbed, but also intact forest communities, rapidly propagating though suckering and hybridizing with native species. Our results suggest that efficient seed dispersal can counterbalance the effects of vegetative propagation, maintaining a high genetic diversity.

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Hua Wang, Dong Pei, Rui-sheng Gu, and Bao-qing Wang

between populations is low. Table 4. Genetic differentiation and gene flow among nine Juglans populations. Genotypic structure. In individual populations, tests for the departure from H-W equilibrium showed significant deviations

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Dong Liu, Ping Li, Jiulong Hu, Kunyuan Li, Zhenyu Zhao, Weiyan Wang, Jinyuan Zhang, Xu Ding, and Zhimou Gao

( H t ), and genetic diversity within groups ( H s ) of P. sojae were computed with POPGENE 1.3.1 software ( Yeh et al., 1999 ). The genetic differentiation coefficient ( G ST ), gene flow coefficient ( N m ), genetic identity (I), and genetic

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David M. Czarnecki II, Madhugiri Nageswara Rao, Jeffrey G. Norcini, Frederick G. Gmitter Jr, and Zhanao Deng

” (presence of bands) for each individual plant was analyzed using population genetics computer programs to calculate the genetic diversity within populations, genetic differentiation among populations, genetic relationships among populations, and historical

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Gayle M. Volk, Christopher M. Richards, Adam D. Henk, Ann A. Reilley, Patrick A. Reeves, Philip L. Forsline, and Herb S. Aldwinckle

, the samples that originated in Armenia had more private alleles than those from Georgia, but allelic richness levels were comparable ( Table 3 ). Genetic differentiation (F st ) was low between the countries of Armenia and Georgia. The level of