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Julio Solis, Arthur Villordon, Niranjan Baisakh, Don LaBonte, and Nurit Firon

classified as U.S. #1 grade, the premium yield grade, was also counted. Gene expression profiling by quantitative reverse transcription PCR (qRT-PCR). RNA was extracted from 2-week-old root tissues using the RNeasy plant minikit (Qiagen, Valencia, CA

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C.D. Kokkinos, C.A. Clark, C.E. McGregor, and D.R. LaBonte

Sweet potato virus disease (SPVD) is the most devastating disease of sweetpotato [Ipomoea batatas (L.) Lam.] globally. It is caused by the co-infection of plants with a potyvirus, sweet potato feathery mottle virus (SPFMV), and a crinivirus, sweet potato chlorotic stunt virus (SPCSV). In this study we report the use of cDNA microarrays, containing 2765 features from sweetpotato leaf and storage root libraries, in an effort to assess the effect of this disease and its individual viral components on the gene expression profile of I. batatas cv. Beauregard. Expression analysis revealed that the number of differentially expressed genes (P < 0.05) in plants infected with SPFMV alone and SPCSV alone compared to virus-tested (VT) plants was only 3 and 14, respectively. However, these findings are in contrast with SPVD-affected plants where more than 200 genes were found to be differentially expressed. SPVD-responsive genes are involved in a variety of cellular processes including several that were identified as pathogenesis- or stress-induced.

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Huiying Li, Hongji Luo, Deying Li, Tao Hu, and Jinmin Fu

enzymes may be used as indicators of the responses in perennial ryegrass to Pb at an early stage of exposure, gene expression profiles provided more accurate time course of the activation of those systems. Literature Cited Alscher, R.G. Erturk, N. Heath, L

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Concetta Licciardello, Biagio Torrisi, Maria Allegra, Fabiola Sciacca, Giancarlo Roccuzzo, Francesco Intrigliolo, Giuseppe Reforgiato Recupero, Paola Tononi, Massimo Delledonne, and Vera Muccilli

to calcareous (c) and volcanic (v) soil. z Differential gene expression profiles specific to calcareous soil. The genes that were differentially expressed in the SC and CC rootstocks grown in calcareous soil were mostly involved in the oxidative

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Jiao Chen, De-bao Yuan, Chao-zheng Wang, Yi-xing Li, Fen-fang Li, Ke-qian Hong, and Wang-jin Lu

Many reports indicate that an abundance of really interesting new gene (RING) play key roles in regulating defense responses against abiotic and biotic stresses in plants. In this study, the cloning and functional characterization of a RING gene, MaRING2, in banana (Musa acuminata) fruit are reported. MaRING2 belongs to the NEP1-interacting protein (NIP) RING-H2 finger protein family. Gene expression profiles revealed that MaRING2 was cold responsive and induced by abscisic acid (ABA) treatment during cold storage. In this study, the MaRING2 under control of the Cauliflower mosaic virus 35S (CaMV 35S) promoter was transformed to tobacco (Nicotiana benthamiana) using agrobacterium (Agrobacterium tumefaciens)-mediated transformation. The resultant MaRING2-overexpressing transgenic plants (35S:MaRING2) exhibited significantly increased tolerance to low temperatures and were hypersensitive to exogenous ABA in terms of germination and early seedling growth. In addition, overexpression of MaRING2 enhanced the expression of stress-responsive genes under normal (before cold stress) or cold conditions. These results demonstrate the biological role of MaRING2 in conferring cold tolerance. Taken together, these results suggest that MaRING2, a C3H2C3-type RING protein, is a positive regulator of the ABA-dependent stress response.

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Yan Xu and Yuejin Wang

Expressed sequence tags (ESTs) constitute a rapid and informative strategy for studying gene-expression profiles of specific stages of annual and perennial plant species. Compared with annual plants, the NCBI database has very little sequence information from perennial plant species. To date, only ∼145 ESTs of Vitis pseudoreticulata W.T. Wang have been deposited in databases. This is insufficient to understand the biology and development of this species. In this report, a cDNA library constructed from young leaf inoculated with powdery mildew pathogen [Uncinula necator (Schw.) Burr.] of Chinese wild Vitis pseudoreticulata. Leaf was harvested at various times after inoculation for total RNA extraction, which was used to generate ESTs. In our study, 107 cDNA clones were sequenced either from 5' or 3' end of the cDNAs. Among them, 60 unigenes (56%) were functionally characterized by the BLASTX matches to known function proteins, and 20 unigenes (18.6 %) matched significantly with those having unknown function in the public databases. The remaining 27 unigenes (25.2%) failed to show significant homology to any proteins in the public databases, suggesting that they represent novel sequences. Some functional genes identified from the cDNA library to be potentially associated with plant defence-related responses are discussed.

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Hadi Susilo and Yao-Chien Alex Chang

and gene expression profiling studies. MS thesis, Natl. Taiwan Univ., Taipei, Taiwan [in Chinese with English abstract] Johnson, C.M. Stout, P.R. Broyers, T.C. Carlton, A.B. 1957 Comparative chlorine requirements of different plant species Plant Soil 8

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Yu Bai, Ying Zhou, Xiaoqing Tang, Yu Wang, Fangquan Wang, and Jie Yang

the gene expression profiles. GO enrichment analysis of the DEGs was implemented by the GOseq R packages based on the Wallenius noncentral hypergeometric distribution ( Young et al., 2010 ). The numbers of all DEGs (upregulated and downregulated) were

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Young-Hwan Shin, Rui Yang, Yun-Long Shi, Xu-Min Li, Qiu-Yue Fu, Jian-Liang Lu, Jian-Hui Ye, Kai-Rong Wang, Shi-Cheng Ma, Xin-Qiang Zheng, and Yue-Rong Liang

in Table 1 . Fig. 4. Relationship of 2-C-methyl- d -erythritol-4-phosphate pathways to carotenoid pathway and chlorophyll pathway. Digital gene expression profiling and quantitative real-time PCR (qRT-PCR) studies show that intensive light inhibits

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Jianfeng Liu, Bowen Yang, Yuetong Ming, Yuchu Zhang, and Yunqing Cheng

Effects of raspberry phytochemical extract on cell proliferation, apoptosis, and serum proteomics in a rat model J. Food Sci. 76 192 198 Cheng, Y. Liu, J. Zhang, H. Wang, J. Zhao, Y. Geng, W. 2015 Transcriptome analysis and gene expression profiling of