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Mónica Moura, Maria Irene Candeias and Luís Silva

Only 7% of the 947 vascular plant taxa given for the Azores Archipelago are considered endemic ( Silva et al., 2005 ). Viburnum treleasei Gand. ( Fig. 1A ), of the Adoxaceae family ( Backlund, 1996 ), is a hermaphroditic shrub or small tree

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Orlanda Cristina Barros Moreira, José Martins, Luís Silva and Mónica Moura

Prunus azorica (Hort. ex Mouillef.) Rivas Mart., Lousã, Fern. Prieto, E. Dias, J.C. Costa, and C. Aguiar, commonly named Azorean cherry or “ginja-do-mato,” is an endangered tree endemic to the Azores, Portugal. It is currently found in São Miguel

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Kelly D. Norris and William R. Graves

, plant vigor, and other traits among the broadly distributed D. palustris and to assess potential for using D. occidentalis and D. mexicana outside of the small areas where they are endemic. We initiated a common-garden experiment to evaluate plants

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Justin A. Porter, Hazel Y. Wetzstein, David Berle, Phillip A. Wadl and Robert N. Trigiano

Georgia plume ( Elliottia racemosa , Ericaceae) is a beautiful, rare, small tree endemic only to Georgia, where it is listed as a threatened species ( Georgia Department of Natural Resources, 2006 ). It produces spectacular plume-like white

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Carrie A. Radcliffe, James M. Affolter and Hazel Y. Wetzstein

thidiazuron J. Amer. Soc. Hort. Sci. 133 167 172 Woo, S.M. Wetzstein, H.Y. 2008b An efficient tissue culture regeneration system for georgia plume, Elliottia racemosa , a threatened Georgia endemic HortScience 43 447 453 Yi, W.G. Law, S.E. McCoy, D. Wetzstein

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Xiao-Xian Li and Zhe-Kun Zhou

Northwestern Yunnan is situated in the southern part of the Hengduan Mountains, which is a complex and varied natural environment. Consequently, this region supports a great diversity of endemic plants. Using field investigation in combination with analysis of relevant literature and available data, this paper presents a regional ethnobotanical study of this area. Results indicated that northwestern Yunnan has an abundance of wild ornamental plants: this study identified 262 endemic species (belonging to 64 genera and 28 families) with potential ornamental value. The distinguishing features of these wild plants, their characteristics and habitats are analyzed; the ornamental potential of most plants stems from their wildflowers, but some species also have ornamental fruits and foliage. Among the endemic genera, Pedicularis and Rhododendron have particularly high numbers of ornamental wild species, while Aconitum, Gentiana, Corydalis, Silene, Delphinium, Cremanthodium, and Saussurea also contain significant numbers of wild ornamental species. It is suggested that cultivation of these species may be beneficial, both commercially and to help conserve endangered endemic plant species.

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María Luisa Osorio-Rosales and Martín Mata-Rosas

Experiments were conducted to establish an efficient protocol of micropropagation of Beaucarnea gracilis and B. recurvata two endemic and endangered Mexican species. Multiple shoots were induced by direct organogenesis from in vitro seedlings and longitudinal sections of seedlings in both species. The highest formation of shoots per explant, both B. gracilis and B. recurvata, was obtained from longitudinal sections of seedlings on Murashige and Skoog (MS) medium supplemented with 22.2 μm 6-benzylaminopurine, induced 8.2 and 11.1 shoots per explant respectively. In vitro rooting was readily achieved on MS medium with 1 g/l activated charcoal without growth regulators. According to initial treatment and depending on where the shoots come from, the rooting rates were 61% to 100% for B. gracilis, and 83% to 100% for B. recurvata. Survival rates in greenhouse conditions for both species were 80% to 100% after 3 months. These results indicate that the micropropagation of these species of Beaucarnea is technically feasible, and that in vitro culture is a useful option for the conservation and propagation of these important endangered species.

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Loreto Araneda, Paulina Salas and Leví Mansur

Few cytological studies have been conducted on the endemic Chilean genus Leucocoryne, which is comprised of 14 species and a number of populations whose taxonomy has not been elucidated. Eleven species/populations of Leucocoryne have been examined cytologically and L. ixioides (Hook.) Lindl., L. coquimbensis var coquimbensis F.Phil., L. narcissoides R. Phil., L. sp. `Alcones', L. sp. `Talinay' and L. sp. `Combarbalá' were observed to be 2n = 18; L. purpurea Gay, L. sp. `Ñague', L. sp. `Alicahue' and L. sp. `Pichicuy' are 2n = 10 and L. coquimbensis var alba had 2n = 14. Furthermore, a natural hybrid population of L. purpurea and L. coquimbensis var coquimbensis had individuals with 2n = 14 and 2n = 22 chromosomes in addition to the normal complement of 2n = 10 and 2n = 18. The results indicate that Leucocoryne with the exception of L. coquimbensis var alba is typically either 2n = 10 or 2n = 18 with a similar number of species or populations at each ploidy level. Furthermore, the hybrid population demonstrated that there is gene flow between the species at different ploidy levels.

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Justin A. Porter, David Berle and Hazel Y. Wetzstein

for ex situ conservation, and exploration for unknown populations. Georgia plume [ Elliottia racemosa (Ericaceae)] is a small tree that is endemic only to the state of Georgia, where it is listed as a threatened species ( Georgia Department of Natural

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Seong Min Woo and Hazel Y. Wetzstein

Georgia plume, Elliottia racemosa Muhlenb. ex. Elliott, is an extremely rare small tree or shrub endemic to Georgia, which is being severely affected by habitat loss and lack of sexual recruitment. In vitro plant regeneration of Georgia plume has not been previously reported and may be a method for the conservation and propagation of this threatened species. Studies evaluated the effects of sterilization methods, explant types, medium composition, and light environment on plant regeneration. An efficient plant regeneration system was developed in which adventitious shoot buds were induced using young, expanding leaf explants placed on an induction medium supplemented with 10 μm thidiazuron and 5 μm indole-3-acetic acid with Gamborg's B5 salts. Shoot elongation was promoted on a medium with 25 μm (2-isopentenyl) adenine incorporated into Woody Plant Medium. In vitro rooting studies evaluated continuous and pulse auxin treatments and ex vitro rooting trials after KIBA (indole-3-butric acid, potassium salt) dips. A 5-day pulse treatment on 100 or 150 μm indole-3-butyric acid produced ≈90% rooting of shoots with greater shoot and root dry weight than other pulse times. High rooting frequencies were obtained under in vitro and ex vitro conditions with over 85% survival of plantlets transferred to greenhouse conditions. The culture protocol was found to be effective with material collected from mature specimens in the wild from divergent populations. Tissue culture appears to be a promising approach for the propagation and conservation of this rare and threatened plant.