Red- and purple-leafed seedlings and clonal material selected for superior color and growth under northern climatic conditions may exhibit progressive color loss and reduced growth rates when exposed to the hot summers and high night temperatures of more southern climates. Studies were conducted to characterize the color loss associated with red-leafed seedlings of Acer palmatum Thunb. (Japanese maple), and to determine to what extent night temperatures affect the dark respiration, growth, and anthocyanin expression of A. palmatum `Bloodgood'. The percentage of seedlings within each of five color classes was determined for five dates from spring to early fall. Significant shifts in class distribution occurred on every evaluation date tested. The class changes contributing the most to these shifts varied with age of leaf material and date. Dark respiration rates increased by 0.09 mg CO2/g leaf dry weight per hour for every 1C rise in temperature, regardless of exposure duration. Dark respiration rates of 0.69 and 1.73 mg CO2/g per hour were found at 14 and 26C, respectively. The greatest amount of growth occurred during weeks 6 through 8 at a night temperature of 14C. Plant growth during this period increased by an average 51%, compared to that at warmer night temperatures. Ultimately, total plant growth at 14C decreased 7%, 19%, and 32% as night temperatures increased from 18 to 22 to 26C. Leaf redness index values at 14 or 18C were from two to seven times greater than those at warmer night temperatures.
D.L. Deal, J.C. Raulston, and L.E. Hinesley
Kathleen B. Evensen and Karen M. Olson
Postproduction quality, net C exchange, and petal abscission in response to ethylene were compared following forcing at 21(day)/16C(night) or 18/13C(18-hour photoperiod) of two cultivars of Pelargonium × domesticum L.H. Bailey. Fewer petals of 2- to 6-day-old florets abscised in response to 60 minutes of 0.7 μl ethylene/liter on plants forced at low temperature than on plants forced at 3C higher temperature. Forcing temperature did not affect floret longevity or the number of florets opening during forcing, but the floral display under simulated consumer conditions was prolonged in low-temperature plants by the continued development of buds. Dark respiration rates at 21C were lower in leaves from plants forced at low temperature than in leaves of plants forced at the higher temperature. Differences in postproduction quality between plants forced at high and low temperatures may have been related to the reduced rate of carbohydrate depletion in low-temperature plants.
Robert H. Stamps, Terril A. Nell, and James E. Barrett
Leatherleaf fern [Rumohra adiantiformis (Forst.) Ching] fronds produced under a high-temperature regime (HTR, 30 day/25C night) grew faster and produced sori earlier than those in a low-temperature regime (LTR, 20 day/15C night). Abaxial diffusive conductance was lower for HTR-grown fronds. Light-saturated net CO2 assimilation rates (Pn) and dark respiration were lower for HTR fronds, but light-saturated Pn efficiencies (chlorophyll basis); light compensation points; and soluble sugars, starch, and nonstructural carbohydrate levels were similar for the two regimes. Transpiration and water-use efficiency (mass basis) at light saturation were similar for fronds from both temperature treatments. Comparison of physiological characteristics of fronds from the two temperature regimes revealed no differences that might account for reduced postharvest longevity of fronds produced at the higher temperatures.
M. Hossein Behboudian and Robert Lai
Responses of the tomato (Lycopersicon esculentum Mill. cv. Virosa) plant to elevated CO2 concentrations applied throughout the photoperiod or part of it were studied under two temperature regimes. Plants were exposed to CO2 at 340 (control), 700, and 1000 μl·liter–1. The highest concentration was applied only at 22/16C (day/night) and 700 μl·liter–1 at 22/16C and 25/16C. Transpiration rates were lower and photosynthetic rates were higher under elevated CO2 than at the ambient level. Biomass production was higher only for plants grown at 700 μl·liter–1 and 25/16C. Concentrations of macronutrients were lower in plants exposed to 1000 μl CO2/liter than in the control plants. Intermittent CO2 was applied using two timing methods. In method 1, plants were exposed to 4- or 8-hour high-CO2 concentrations during their 12-hour photoperiod. In method 2, plants were exposed for 3.5 days of each week to 700 μl CO2/liter. Only two of the 8-hour exposures resulted in greater growth than the controls. The lack of higher growth for CO2-enriched plants at 22/16C was attributed to a higher dark respiration rate and to a lack of efficient transport of photosynthates out of leaves.
Terril A. Nell, Ria T. Leonard, and James E. Barrett
Production irradiance levels on growth, light compensation point (LCP), dark respiration (DR), and interior longevity of potted chrysanthemum (Demfranthema grandiflora Tzvelev. cvs. Iridon and Mountain Peak) and poinsettia (Euphorbia pulcherrima Wind. cvs. Annette Hegg Dark Red and Gutbier V-10 Amy) were determined. LCP and DR were measured at anthesis and during acclimatization to interior conditions (10 μmol·s-1·m-2). Days to flowering, inflorescence diameter, total chlorophyll, and interior longevity of chrysanthemum increased when maintained at a mean maximum photosynthetic photon flux density (PPFD) of 500 μmol·s-1·m-2 compared to plants shifted to 300 or 100 μmol·s-1·m-2 8 weeks after planting. LCP and DR were highest at anthesis and were reduced 38% and 49%, respectively, for chrysanthemum and 19% and 42%, respectively, for poinsettia within 3 days in interior conditions. Chrysanthemum plants shifted to 300 μmol·s1·m-2 during production had lower LCP and DR rates at anthesis and throughout time in interior conditions compared to plants maintained at 500 μmol·s-1·m-2. The acclimatization of chrysanthemum to reduced production PPFD is of little significance because interior longevity is reduced. No differences were found in the LCP or DR of poinsettia or chrysanthemum cultivars that differ in interior performance, demonstrating that these physiological characteristics are not good indicators of interior longevity for chrysanthemum and poinsettia.
David C. Percival, J.T.A. Proctor, and M.J. Tsujita
The influence of irradiance, CO2, and temperature on whole-plant net CO2 exchange rate (NCER) of Rubus idaeus L. `Heritage' micropropagated raspberries was examined. Within the set of environmental conditions examined, irradiation was the most important factor, accounting for 58% of the whole-plant irradiance/CO2 concentration/temperature NCER model variation, followed by CO2 concentration (28%) and temperature (2.5%). Net photosynthesis (Pn) required irradiance levels >600 μmol·m-2·s-1 PPF for saturation, greatly increased under CO2 enrichment (up to 1500 μL·L-1), and was optimum at a whole-plant temperature of 20 °C. Temperature effects were partitioned in an experiment using varying air and root-zone temperatures (15, 20, 25, 30, and 35 °C) under saturated light and ambient CO2 levels (350 μL·L-1). Air and root-zone temperature influenced Pn, with maximum rates occurring at an air × root-zone temperature of 17/25 °C. The contribution of air and root-zone temperature to the NCER model varied, with air and root-zone temperature contributing 75% and 24%, respectively, to the total model variation (R 2 = 0.96). Shoot dark respiration increased with air and root-zone temperature, and root respiration rates depended on air and root-zone temperature and shoot assimilation rate. Humidity also influenced Pn with a saturated vapor pressure deficit threshold >0.25 kPa resulting in a Pn decrease. Quantifying the physiological response of raspberries to these environmental parameters provides further support to recent findings that cool shoot/warm root conditions are optimum for raspberry plant growth.
Marc W. van Iersel and Jong-Goo Kang
To determine the effect of fertilizer concentration on plant growth and physiology, whole-plant C exchange rates of pansies (Viola ×wittrockiana Gams.) subirrigated with one of four fertilizer concentrations were measured over 30 days. Plants were watered with fertilizer solutions with an electrical conductivity (EC) of 0.15, 1.0, 2.0, or 3.0 dS·m-1 (N at 0, 135, 290, or 440 mg·L-1, respectively). Plants watered with a fertilizer solution with an EC of 2 dS·m-1 had the highest shoot dry weight (DW), shoot to root ratio, leaf area, leaf area ratio (LAR), and cumulative C gain at the end of the experiment compared to those watered with a solution with a higher or lower EC. Shoot tissue concentrations of N, P, K, S, Ca, Fe, Na, and Zn increased linearly with increasing fertilizer concentration. A close correlation between final DW of the plants and the measured cumulative C gain (CCG) (r2 = 0.98) indicated that the C exchange rates were good indicators of plant growth. There were quadratic relationships between fertilizer EC and gross photosynthesis, net photosynthesis, and dark respiration, starting at 13, 12, and 6 days after transplanting, respectively. Although plants fertilized with a fertilizer solution with an EC of 2 dS·m-1 had the highest C exchange rates, the final differences in shoot DW and CCG among ECs of 1.0, 2.0, and 3.0 dS·m-1 were small and it appears that pansies can be grown successfully with a wide range of fertilizer concentrations. Plants with a high LAR also had higher DW, suggesting that increased growth was caused largely by increased light interception. A detrimental effect of high fertilizer concentrations was that it resulted in a decrease in root DW and a large increase in shoot to root ratio.
Richard J. Campbell, Richard P. Marini, and Jeffrey B. Birch
Light response curves for gas exchange characteristics were developed for spur leaves of `Stayman' and `Delicious' apple (Malus domestica Borkh.) from interior, intermediate, and exterior canopy positions throughout the season. At full bloom (FB), before full leaf expansion, exterior leaves had higher maximum rates of net photosynthesis (Pn), and a statistically different Pn light response curve than the interior leaves. Intermediate leaves had intermediate Pn rates and light response curves. Pn light response curves for all three `Delicious' canopy positions differed from each other from FB + 6 weeks until the end of the season. Interior leaves had maximum Pn rates of only 50% to 60% of those for the exterior leaves from FB + 10 weeks until the end of the season. Light saturation levels were higher for the exterior leaves than for interior or intermediate leaves. Exterior leaves had a tendency throughout the season for higher quantum efficiency of Pn at subsaturating light levels than interior or intermediate leaves. Stomatal conductance was higher for the exterior than the interior or intermediate leaves of `Delicious' on all dates. Water-use efficiency was equivalent among all leaves. Exterior leaves had higher specific leaf weight, dark respiration rates, and incident light levels on all dates than interior or intermediate leaves.
Sydney Lykins, Katlynn Scammon, Brian T. Lawrence, and Juan Carlos Melgar
photosynthesis ( P Nmax ), dark respiration ( R D ), light compensation point ( I comp ), and light saturation point ( I max ). The P N values at PH or AH did not decline with increasing irradiance, with the exception of the PH curve of ‘Natchez’, which
Thomas E. Marler and Yasmina Zozor
Leaf gas exchange, chlorophyll fluorescence, water relations, and mineral nutrient relations responses of Annona squamosa seedlings to mild salinity were studied in sand culture in five experiments during 1990, 1991, and 1993. Trees were irrigated with a complete nutrient solution (control) or with this solution amended to 3 or 6 dS·m-1 with sea salt. Inhibition of net CO2 assimilation, stomatal conductance of CO2, and transpiration was apparent within 2 weeks of initiating salinity treatments, and gas exchange continued to decline until day 30 to 35. The diurnal pattern of leaf gas exchange was not altered by increased salinity. Salinity reduced CO2, light energy, and water-use efficiencies. Salinity sometimes reduced the ratio of variable to maximum fluorescence below that of the control, and this response was highly dependent on the ambient light conditions that preceded the measurements. Dark respiration was unaffected by salinity stress. Root zone salinity of 3 dS·m-1 administered for 52 days did not influence foliar sodium concentration or the ratio of sodium to potassium, but increased chloride concentration and decreased nitrogen concentration. The sodium response indicated that some form of exclusion or compartmentation occurred. Salinity reduced osmotic potential of root tissue but did not influence foliar osmotic or predawn xylem potential. These results indicate that A. squamosa is sensitive to salinity stress, and that the responses to salinity are consistent with other salt-sensitive woody perennial species.