gender differentiation mechanism in Vitis species. In the present study, we 1) focused on the abortion of pistil and its recovery from the perspective of cytology and 2) aimed to provide a more theoretical basis for gender determination mechanism in
pollen viability was low. Nonetheless, the structural events of anther development involved in petaloid-type male sterility in C. oleifera remain unknown. Therefore, in our study, we examined the anther structure and pollen morphology for a cytological
pollen and cytological traits. The objectives of this study are to characterize fully the chromosome and pollen morphology, pollen stainability, and nuclear DNA content of gulf vervain. Materials and Methods Plant materials. Gulf vervain
biomass and to improve medicinal and ornamental characteristics. In the present study, the induction of polyploid on feverfew was examined and morphological, physiological, cytological, and phytochemical characteristics of diploid and induced
., 2012 ; Deng et al., 2017 ). The objective of this study was to assess morphological and cytological differences among eight trailing lantana varieties collected from different growers and a naturalized area in Texas and Australia. Information in these
were examined by ESEM (Quanta 200; FEI, Eindhoven, The Netherlands) in their natural form without additional sample preparation. Results Cytological characteristics of embryonic callus cells. Histologically, embryonic calli were composed of two
can provide greater insight into a genus and thus aid in developing breeding strategies. The base chromosome number of Acer is x = 13. Cytological reports for maples include a range of ploidy levels ( Darlington and Wylie, 1956 ). The greatest
alter breeding strategy ( Fehr, 1991 ). Unfortunately, relative to the number of species in the genus, the cytological information is sparse for Callicarpa . The first beautyberry chromosome count reported was for C. japonica (2 n = 32) by Sugiura
pair composition was calculated as GC% = 100 – AT%. Relative genome size was measured on a flow cytometer (CyFlow Ploidy Analyzer, Sysmex) with fluorescence excitation of 365 nm for DAPI-stained samples and 532 nm for PI-stained samples. Cytology
Clear visualization of asparagus (Asparagus officinalis L.) microspore nuclei with common stains such as acetocarmine or DAPI is difficult, hindering cytological analyses. The addition of saturated aqueous ferric chloride solution to Carnoy's I fixative (30 μL·mL-1) resulted in clear visualization of nuclei. A distinct nucleus was observed in uninucleate cells and the vegetative and generative nuclei were clearly visible in binucleate microspores. This method can be used reliably for determination of asparagus microspore developmental stage. Chemical name used: 4′,6-diamidino-2-phenylindole-2HCL (DAPI).