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Jason Cavatorta, George Moriarty, Mark Henning, Michael Glos, Mary Kreitinger, Henry M. Munger, and Molly Jahn

‘Marketmore 76’ has been widely planted since it was first introduced 30 years ago and represents one of the most successful cucumber cultivars ever released. Despite the subsequent development of numerous higher-yielding hybrids ( Rowell et al

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Min Wang, Wenrui Liu, Biao Jiang, Qingwu Peng, Xiaoming He, Zhaojun Liang, and Yu’e Lin

., 2006 ) and heat shock transcription factors (HSFs) ( Panchuk et al., 2002 ), and the HS signal transduction pathway ( Joyce et al., 2003 ; Suzuki and Mittler, 2006 ). Cucumber ( Cucumis sativus L., 2 n = 2 x = 14) is a widely cultivated plant in the

Open access

Xiaotao Ding, Liyao Yu, Yuping Jiang, Shaojun Yang, Lizhong He, Qiang Zhou, Jizhu Yu, and Danfeng Huang

Cucumber ( Cucumis sativus ) is among the most widely grown vegetables in the world and is native to the Indian subcontinent ( Sebastian et al., 2010 ). It spread eastward to China ≈2000 years ago and westward to Europe 700 to 1500 years ago ( Weng

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Xiaotao Ding, Yuping Jiang, Dafeng Hui, Lizhong He, Danfeng Huang, Jizhu Yu, and Qiang Zhou

satisfy the heating requirements while keeping the environmental conditions as favorable as possible for plant growth ( Boulard et al., 2017 ). Cucumber ( Cucumis Sativus L.) is a popular vegetable in China and considered as a favorite greenhouse crop

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Marivi Colle, Elizabeth N. Straley, Stephanie B. Makela, Sue A. Hammar, and Rebecca Grumet

Cucumber ( Cucumis sativus ) production in the eastern and midwestern United States is subject to severe losses resulting from fruit rot caused by the soilborne oomycete pathogen, Phytophthora capsici ( Granke et al., 2012 ; Sonogo and Ji, 2012

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Paula P. Chee and Jerry L. Slightom

Abbreviations: Cb, carbenicillin; CMV, cucumber mosaic virus; GUS, (β -glucuronidase; Km, kanamycin; MS, Murashige and Skoog NPT II, neomycin phosphotransferase II; NOS, nopaline synthase. We thank Krystal A. Fober for plant care in the greenhouse

Open access

Bao-Zhong Yuan, Zhi-Long Bie, and Jie Sun

The cucumber ( Cucumis sativus L.), which belongs to the Cucurbitaceae family, is a commonly consumed vegetable. It is an economically important crop that is widely cultivated throughout the world. Cucumber plants often experience biotic and

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F. Gilbert-Albertini, M. Pitrat, and H. Lecoq

1 To whom reprint requests should be addressed. We thank R. Provvidenti for providing `Taichung Mou Gua' cucumber seeds and for a critical review of this manuscript. The cost of publishing this paper was defrayed

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Wenjing Guan, Daniel S. Egel, Larry D. Sutterer, and Alexander D. Plummer

Seedless cucumber, also known as greenhouse cucumber, is a popular and high-value crop found in many local food markets. Like tomato, cucumber is sold for premium prices in early-season markets ( Curtis et al., 2012 ). Seedless cucumber plants are

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Prem Anand Rajagopalan and Rafael Perl-Treves

Agrobacterium-mediated transformation of Cucumis sativus L. cotyledons was investigated, to identify important factors that affect transformation efficiency. The factors evaluated were initial explant preparation, including preculture, pricking of the explant, inoculation time and co-cultivation regime. We also modified the selection regime, compared to previously published protocols. Our results show that dissecting the proximal half of the cotyledon by a V-shaped cut resulted in a higher transformation rate, compared to two other methods of dissection, as indicated by transient β-glucuronidase gene expression. Selection on 100 mg·L-1 kanamycin resulted in the early development of nontransformed shoots, whereas a gradual increase of kanamycin concentration up to 200 mg·L-1 resulted in the subsequent formation of transgenic shoots on the same medium. The overall transformation frequency in these experiments, expressed as the number of rooted, confirmed transgenic plants per initial number of explants, was 1.7%. The stable integration of T-DNA was confirmed in the primary transformants and their progeny. Abbreviations: ABA = abscisic acid; AdS = adenine sulfate; AS = acetosyringone; BA = 6-benzylaminopurine; GA3 = gibberellic acid; IBA = indolebutyric acid.