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Alan T. Whittemore and Alden M. Townsend

. Table 1. Plants used as parents in crossing experiments. Controlled crosses. About a month before normal anthesis, mature branches were brought inside the greenhouse to force pollen shed. Pollen was collected on waxed paper, transferred

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Tera M. Bonney, Shawn P. Brown, Snake C. Jones, Kirk W. Pomper, and Robert L. Geneve

The pawpaw [Asimina triloba (L.) Dunal] is a native plant found mainly in the southeastern and eastern United States, and its fruit has great potential as a new high-value crop in these regions. Although there are ≈45 named pawpaw cultivars, breeding for improvement of specific traits, such as fruit size and quality, is desirable. Our long-term goal is to utilize molecular marker systems to identify markers that can be used for germplasm diversity analyses and for the construction of a molecular genetic map, where markers are correlated with desirable pawpaw traits. The objective of this study was to identify random amplified polymorphic DNA (RAPD) markers that segregate in a simple Mendelian fashion in a controlled A. triloba cross. DNA was extracted from young leaves collected from field-planted parents and 20 progeny of the cross 1-7 × 2-54. The DNA extraction method used gave acceptable yields of ≈7 μg·g-1 of leaf tissue. Additionally, sample 260/280 ratios were ≈1.4, which indicated that the DNA was of high enough purity to be subjected to the RAPD methodology. Screening of 10-base oligonucleotide RAPD primers with template DNA from the parents and progeny of the cross has begun. We have identified two markers using Operon primer B-07 at 1.1 and 0.9 kb that segregate in a simple Mendelian fashion in progeny of the 1-7 × 2-54 cross. Other primers and controlled crosses will also be screened.

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Jason D. Lattier and Ryan N. Contreras

-pollinations were determined to be negligible during controlled crosses. Viable seedlings across all taxa exhibited a quiescent phase of vegetative growth during their first year. During this period, seedlings produced few sets of leaves while they developed an

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Rachid Mentag, Isabelle Duchesne, and Jacques-André Rioux

29 POSTER SESSION 3 Weed Control/Cross-Commodity

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N.G. Creamer, M.A. Bennett, J. Cardina, and E.E. Regnier

29 POSTER SESSION 3 Weed Control/Cross-Commodity

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Neil O. Anderson, Peter D. Ascher, and Barbara E. Liedl

29 POSTER SESSION 3 Weed Control/Cross-Commodity

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Steven D. Siegelin, Darrel D. Daniels, Merrill A. Ross, and Stephen C. Weller

29 POSTER SESSION 3 Weed Control/Cross-Commodity

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Milton E. McGiffen Jr., E.J. Ogbuchiekwe, and B.S. Saharan

29 POSTER SESSION 3 Weed Control/Cross-Commodity

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J. Gill, C. Laguë, N. Lehoux, G. Péloquin, J. Coulombe, and S. Yelle

29 POSTER SESSION 3 Weed Control/Cross-Commodity

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Anusuya Rangarajan, A. Raymond Miller, and Richard Veilleux

29 POSTER SESSION 3 Weed Control/Cross-Commodity