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Na-Hyun Song and Yeh-Jin Ahn

chinensis L.) was not expressed in the absence of stress but was induced under a cold stress condition (2 °C, 6 h; Jiang et al., 2009 ). These studies suggest that at least some plant sHSPs may confer cold tolerance. A number of studies have shown that

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Jibiao Fan, Jing Ren, Weixi Zhu, Erick Amombo, Jinmin Fu, and Liang Chen

) ( Hughes and Dunn, 1996 ). Some studies have reported that cold stress led to biochemical and physical changes in plants, particularly causing freezing injury, which was accounted for by the damage on the plasma membrane. Other studies found that low

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Ren-jun Feng, Li-li Zhang, Jing-yi Wang, Jin-mei Luo, Ming Peng, Jun-feng Qi, Yin-don Zhang, and Li-fang Lu

Cold stress is one of the major environmental factors that often affects crop growth, productivity, quality, and postharvest life ( Sanghera et al., 2011 ). Cold stress can be classified as chilling (0 to 15 °C) or freezing (<0 °C) stress. To cope

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J. D. Norton, C. E. Boyhan, Hongwen Huang, and B. R. Abrahams

On March 13-15, 1993 Alabama and much of the eastern United States experienced an unusually severe winter storm. This afforded the evaluation of plum cultivar production under cold stress. The highest yielding variety that bloomed before the storm was Bruce 12-4 with 28 kg/tree. Bruce 12-4 is noted for blooming over an extended period of time and producing very heavy yields. The average yield of the top five performers that bloomed after the storm was 51 kg/tree. The lowest temperature recorded at the test site, Shorter, AL was -5C.

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Jiao Chen, De-bao Yuan, Chao-zheng Wang, Yi-xing Li, Fen-fang Li, Ke-qian Hong, and Wang-jin Lu

membrane trafficking and modulated salt-stress response in arabidopsis ( Tian et al., 2015 ). The maize ( Zea mays ) RING-H2 finger protein gene ZmXERICO was up-regulated in salt, drought, abscisic acid, and cold stresses ( Gao et al., 2012 ). A Brassica

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Jean-Pierre Privé and M.I.N. Zhang

2,3,5-triphenyl tetrazolium chloride (TTC) staining, electrical conductivity, and electrical impedance (Z) analyses were used to assess freezing injury of `Beautiful Arcade' apple (Malus ×domestica Borkh.) roots taken in late March from either the field or 3C-refrigerated storage (cold-stored). Lethal temperature (LT50) levels using TTC or electrical conductivity occurred at colder temperatures than those found using Z. Techniques varied in their ability to detect changes in cell viability with increasing cold stress. Listed in order of decreasing responsiveness they are electrical impedance (Z), electrical conductivity, and TTC vital staining. With the most sensitive technique, Z, two parameters—extracellular and total tissue electrical resistance—were about five and eight times lower (indicating more injury) for roots from the field than from cold storage. The smaller values obtained from the field roots were probably due to natural in-field freeze-thaw cycling before the controlled cold-stress tests in the laboratory. More importantly, the impedance technique provided more detailed information than TTC or electrical conductivity about how apple roots respond to cold stress and how Z may provide some insight into freeze-thaw history before injury assessment. Although this technique shows potential, future studies are required to render a complete physiological significance to the impedance parameters involved in calculating Z.

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Mohamad-Hossein Sheikh-Mohamadi, Nematollah Etemadi, and Mostafa Arab

fescue is commonly used for grassland rehabilitation on light-textured soils in shrub lands and rangelands where precipitation is generally low ( Pan et al., 2013 ). Heat and cold stress are two major abiotic factors that limit plant growth and reduce the

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Anik L. Dhanaraj*, Janet P. Slovin, and Lisa J. Rowland

To gain a better understanding of changes in gene expression associated with cold stress in the woody perennial blueberry (Vaccinium spp.), a genomics approach based on the analysis of expressed sequence tags (ESTs) was undertaken. Two cDNA libraries were constructed using RNA from cold acclimated (mid winter conditions when the plants are cold stressed) and non-acclimated (before they received any chilling) floral buds of the blueberry cultivar Bluecrop. About 600 5'-end ESTs were generated from each of the libraries. Putative functions were assigned to 57% of the cDNAs that yielded high quality sequences based on homology to other genes/ESTs from Genbank, and these were classified into 14 functional categories. From a contig analysis, which clustered sequences derived from the same or very similar genes, 430 and 483 unique transcripts were identified from the cold acclimated and non-acclimated libraries, respectively. Of the total unique transcripts, only 4.3% were shared between the libraries, suggesting marked differences in the genes expressed under the two conditions. The most highly abundant cDNAs that were picked many more times from one library than from the other were identified as representing potentially differentially expressed transcripts. Northern analyses were performed to examine expression of eight selected transcripts and seven of these were confirmed to be preferentially expressed under either cold acclimating or non-acclimating conditions. Only one of the seven transcripts, encoding a dehydrin, had been found previously to be up-regulated during cold stress of blueberry. This study demonstrates that analysis of ESTs is an effective strategy to identify candidate cold-responsive transcripts in blueberry.

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Robert J. Dufault and Regina R. Melton

Tomato seedlings (Lycopersicon esculentum Mill. `Sunny') were exposed to cyclic cold stress at 2 ± 1C, then to 29 ± 6C in a greenhouse before being transplanted to the field. Cold-stressed seedlings were transplanted when the risk of ambient cold stress was negligible. In the first year of a 2-year study, transplants were exposed to 2C for 3, 6, or 12 hours for 1, 3, or 6 days before field planting. In the second year, transplants were exposed to 2C for 6, 12, or 18 hours for 4, 7, or 10 days before field planting. In the first year, cold stress generally stimulated increases in seedling height, leaf area, and shoot and root dry weights but decreased chlorophyll content. In the second year, all seedling growth characteristics except leaf area and plant height were diminished in response to longer cold-stress treatment. In both years, earliness, total productivity, and quality were unaffected by any stress treatment. Therefore, cold stress occurring before transplanting has a negligible effect on earliness, yield, or quality.

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Jean-Pierre Prive and M.I.N. Zhang

2,3,5-triphenyltetrazolium chloride (TTC) staining and electrical impedance (?) analyses of apple roots (Malus domestica Borkh. `Beautiful Arcade') taken in late March from either the field or from 3C refrigerated storage (cold-stored). LT50 levels using TTC were much lower than those found using electrical impedance. No loss of viability in the roots was detectable using TTC staining until a freeze–thaw stress of –9C whereas? analysis detected changes in cell viability after a freeze–thaw stress of only –3C. With increasing cold stress, two parameters: extracellular electrical resistance (Ro) and time constant?, decreased linearly for cold-stored roots and exponentially for field roots. Impedance analysis also revealed that the values for both extracellular Ro and total tissue electrical resistance (R?) for the field roots were approximately 5 and 8 times lower, respectively, than in the cold-stored roots. It is believed that the smaller Ro and R? values obtained from the field roots were due to natural in-field freeze–thaw cycling prior to the controlled stress tests in the laboratory. Based on the analyses of winter hardiness using the two methods, the impedance technique? provided the physiological information not only about the hardiness level, but also about freeze–thaw history prior to the hardiness assessment.