Cleistogamy in Salpiglossis sinuatu L. involves a sequence of events, including arrested corolla development, precocious pollen germination inside anther, pollen tube penetration of the pistil, and eventual self fertilization, that takes place. within a tightly closed flower bud. A single dominant gene (C) controls cleistogamy in this plant. During early blooming period, cleistogamous (CC, Cc) plants produce both chasmogamous (open) and cleistogamous (closed) flowers. Enzymes in various tissues of both cleistogamous and chasmogamous buds were detected by isozyme banding patterns in starch gel electrophoresis. The onset of cleistogamy may be signalled in the calyx and corolla tissues in the early stage of flower development. The levels of specific enzymes (PGM, PGI, G-6PD, PGD, MPI) involved in gluconeogenesis, pentose phosphate shunt and glycolysis in both calyx and corolla tissues of the cleistogamous buds were greatly reduced. These enzymes were present in the pistil and anthers of cleistogamous buds and in all floral parts of the chasmogamous buds.
Lijuan Wang, Nian-Oine Shi, Murray E. Duysen, and Chiwon W. Lee
Thomas M. Gradziel and Steven A. Weinbaum
The regulation of anther dehiscence by relative humidity (RH) was assessed for detached anthers and detached whole flowers from a limited selection of apricot (Prunus armeniaca L.), peach [P. persica (L.) Batsch], and almond [P. dulcis (Mill.) D.A. Webb, syn. P. amygdalus Batsch; P. communis (L.) Arcangeli, non Huds.] genotypes, as well as an almond X peach F2 progeny. Dehiscence was evaluated at 33, 64, 87, 93 and 97% RH for detached anthers, and at 33, 64 and 97% RH for whole detached flowers. Anther dehiscence was suppressed with increasing RH for all genotypes. Apricot anthers showed the greatest dehiscence at low RH and measurable dehiscence at high RH even when detached. Anther dehiscence in almond appeared more suppressed than in apricot at all RH levels tested, being completely suppressed by high RH in detached anthers. Peach genotypes exhibited the full range of variability between apricot and almond patterns. Evidence for transgressive segregation of RH-controlled anther dehiscence was observed in the occurrence of cleistogamy in an almond × peach F2 progeny. Rates of anther dehiscence were approximately linear with change in RH in detached anthers but exhibited a more buffered, step-wise response when detached whole flowers were tested. Results are consistent with field observations, and highlight the low but measurable risk of cleistogamy in these species, as well as opportunities to modify the breeding systems and crossing environments to facilitate controlled hybridization, and to reduce pollination vulnerability to adverse environments.
Chiwon W. Lee
Velvet flower (Salpiglossis sinuata, Solanaceae) can be used as an excellent demonstration plant for horticultural crop breeding classes. Salpiglossis produces large trumpetlike flowers exhibiting an assortment of corolla colors and pigmentation patterns. The pistil is large (3 to 4 cm or 1.2 to 1.6 inches long) with a sticky stigmatal tip and flowers can be easily emasculated prior to anthesis. The large pollen grains are shed in tetrads which can be separated and placed on the stigmatal surface. It takes eight to nine weeks from seeding to blooming, with a prolific flowering cycle that comes in flushes. Numerous seeds (about 750 per capsule) are obtained in three weeks after self- or cross-pollination. The influences of three genes that control flower color and pigmentation pattern can be conveniently demonstrated with their dominant and recessive alleles. The R gene controls flower color with red (RR or Rr) being dominant over yellow (rr). The D gene controls the density of pigmentation with solid (DD or Dd) color being dominant over dilute (dd) color. Corolla color striping is controlled by the St gene with striped (stst) being recessive to nonstriped (StSt or Stst) pattern. By using diploid lines of genotypes RRDD (red, solid), RRdd (red, dilute), or rrdd (yellow, dilute) and their crosses, students can easily observe a dominant phenotypic expression in the F1 hybrid and the digenic 9:3:3:1 segregation ratio in the F2 progeny. Another gene (C) that controls flower opening can also be used to show its influence on cleistogamous (closed, selfpollinated, CC or Cc) versus normal chasmogamous (open-pollinated, cc) corolla development. In addition, the induction and use of polyploid (4x) plants in plant breeding can also be demonstrated using this species.
Homer T. Erickson, James Steadman, Chi Won Lee, and Jules Janick
A germplasm release representing 8 selections of Salpiglossis sinuata L. (velvet flower), all free of the cleistogamous character which inhibits corolla development, is being made by the Purdue University Agricultural Experiment Station. Five of the selections are diploid (2n = 44) and 3 are tetraploid (2n = 88).
Luping Qu and Mark P. Widrlechner
not be overlooked in ex situ germplasm conservation ( Sackville Hamilton et al., 2003 ).Variation in plant sexual breeding systems ranges from cleistogamy and in-bud pollination, mechanisms to ensure self-pollination, to mechanisms that foster cross
Michael A. Schnelle
_304011124 > Uttal, L.J. 1963 Cleistogamy in Salvia lyrata Castanea 28 4 162 164 Valery, L. Fritz, H. Lefeuvre, J.-C. Simberloff, D. 2009 Invasive species can also be native Trends Ecol. Evol. 24 585 doi: https://doi.org/10.1016/j.tree.2009