conditions before transplantation. Although few studies have focused on papaya grafting ( Allan et al., 2010 ; Van-Hong and Chung-Ruey, 2018 ), it is generally agreed that the tongue approach and cleft grafting methods are the most appropriate grafting
Juan Carlos Álvarez-Hernández, Javier Zaragoza Castellanos-Ramos and Cesar Leobardo Aguirre-Mancilla
Haley Hibbert-Frey, John Frampton, Frank A. Blazich, Doug Hundley and L. Eric Hinesley
-order” to keep the treatments balanced. Grafting was divided equally between two people. One person had 5 years of grafting experience; the other had 1 year of experience. Scions were grafted to rootstocks using a cleft graft ( Hinesley and Frampton, 2002
Bizhen Hu, Mark A. Bennett and Matthew D. Kleinhenz
Precision Balance; Mettler Toledo)] after drying in an oven (Isotemp™; Fisher Scientific, Waltham, MA) at 50 °C for 2 d. Plants representing 90 rootstock–scion combinations (18 rootstocks × 5 scions) were cleft-grafted as outlined previously ( Bumgarner and
Katherine Kelly Stephenson, John R. Stommel and Timothy J Ng
A protocol was developed to make in vitro graft unions among Lycopercicon spp., and regenerates from cultured graft unions were evaluated for chimera formation. Young seedlings were preconditioned for 4 to 6 days in liquid 1/2-strength Murashige & Skoog (MS) basal medium supplemented with 8.9 μM benzyladenine and 1.0 μM indole-3-butyric acid. Preconditioned seedlings exhibited increased biomass and enhanced graft union survival. In particular, survival of cleft grafts increased from 37% to 95% with the seedling preconditioning. When graft unions among different genotypes were excised from apex-to-apex in vitro cleft grafts and plated on MS basal medium supplemented with 9.1 μM zeatin and 3.9 μM ancymidol, as many as 100 plantlets were regenerated from a single graft union. However, no chimeric regenerates were recovered, indicating that asymmetric responses to grafting may be a limiting factor to in vitro chimera formation.
Elisa Mihovilovich, Humberto A. Mendoza and Luis F. Salazar
Combining ability for resistance to Sweetpotato Feathery Mottle Virus (SPFMV) was evaluated in seven sweetpotato [Ipomoea batatas (L.) Lam] clones. A diallel mating design was used, which resulted in 16 full-sib families. Families were evaluated for SPFMV resistance under greenhouse conditions in a randomized complete-block design. Resistance was tested by grafting Ipomoea nil `Scarlet O' Hara' infected with the russet crack strain of SPFMV (RC-SPFMV) onto individual plants of the families being evaluated. Symptomless plants were further indexed by cleft grafting virus-free Ipomoea setosa Ker plants onto the tested plants. Those plants in which the virus was not recovered by this test were considered resistant. Analysis of variance for SPFMV resistance revealed significant general combining abilities (GCA). Two clones, DLP-886 and TN90.300, exhibited significant positive GCA for SPFMV resistance. No significant specific combining abilities (SCA) were detected among the crosses. Breeding for resistance to SPFMV should focus on careful selection of resistant parents. In addition, results suggest that additive gene action is important in resistance to SPFMV.
W. R.(Bill) Jester, Charles W. Averre and Jonathan R. Schultheis
Russet crack-like symptoms have been observed with increasing frequency on Beauregard storage roots in North Carolina and resulted in some crop failures in neighboring states. The objective of this experiment was to determine if this cracking disorder was soil-borne, seed transmissible or transmissible via grafting. Beauregard plants were obtained from cuttings from commercially available virus-indexed micropropagated plants (M), and selected symptomatic roots (culls) originating from 1992 Foundation stocks (R). In a third treatment plants from each source were alternated in a row, then M and R plants were cleft grafted. The planting was made June 30, 1993 and replicated five times (12 plants per rep). Yield was determined and roots from each hill were washed and examined for russet crack-like symptom(s), and interior color on the proximal end. M roots had 82% good color; while R roots had 19%. M plants contained 0.3% symptomatic roots; R plants 65.5%. Similarly, only 1.6% of the M plants contained a symptomatic root, while 95.0% of the R plants had symptomatic roots. One-third of the grafted M plants contained one or more roots with cracking symptoms. M outyielded R. The russet crack-like disorder was determined to not be soil-borne, but was transmissible through the seed or grafting.
David W. Carey, Mary E. Mason, Paul Bloese and Jennifer L. Koch
, this rate declined to 12% in the second year of their study. In 2002–04, our attempts at traditional grafting of American beech using both side veneer and top cleft graft methods yielded an overall take rate of 19%. In 2002, we contracted a nursery to
Yi-Chen Chen, Wei-Chun Chang, San-Tai Wang and Shu-I Lin
seedling-grafted for commercial production. Based on a literature survey, we found that cabbage can be grafted onto kale ( B. oleracea Acephala group) or kohlrabi ( B. oleracea Gongylodes group) rootstock successfully by cleft grafting, but the survival
Hisayuki Kudo and Takeo Harada
potato plants ≈8 weeks old were used for grafting. Cleft grafting was carried out as follows: rootstocks from Me tomato were prepared by cutting off the shoot at ≈7 cm (below the cotyledons) leaving a stem with no leaves or lateral buds. The diameter of
Richard L. Hassell, Frederic Memmott and Dean G. Liere
The initial grafting method used for melon was cleft grafting ( Ishibashi, 1959 ), but after the introduction of the tongue approach grafting method, its use diminished greatly. The tongue approach method became widespread in Asia because of its