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S.E. Gardiner, H.C.M. Bassett, C. Madie, and D.A.M. Noiton

Information about a rare allele of phosphoglucomutase (PGM) that is shared by `Braeburn' and 16% of cultivars in the New Zealand Cultivar Collection was combined with historical information about cultivar distribution to select a set of 15 cultivars for a more detailed genetic analysis of their relatedness to the key New Zealand apple (Malus domestica Borkh.) `Braeburn'. DNA from all 16 cultivars was examined by RFLP analysis using 41 probe-enzyme combinations and also by RAPD analysis with 39 selected primers. The RFLP and RAPD data excluded a proposal that `Lady Hamilton' and `Braeburn' are genetically identical. All cultivars except `Lady Hamilton' were excluded as potential parents for `Braeburn' based on incompatible RFLP banding. Assessment of genetic distances between `Braeburn' and the other 15 cultivars from RFLP and RAPD data demonstrated that `Lady Hamilton' was more closely related to `Braeburn' than all others. We conclude that there is a high likelihood that `Lady Hamilton' is one of the parents of `Braeburn'.

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C.S. Prakash, O. Zheng, and A. Porobodessai

Stable, transgenic, sweetpotato plants have been developed using an improved somatic embryogenesis consisting of l) stage I—explants incubated in darkness for 14 days on MS medium with 2,4D (2.5 mg·liter–1) and 6-BAP (0.25 mg·liter–1) and 2) stage II—culture in light for 14 to 28 days on MS medium with ABA (2.5 mg·liter–1). Petiole or leaf explants of the genotype PI318846-3 were co-cultivated with Agrobacterium tumefaciens EHA 101 containing gusA::nptII fusion gene. Transgenic somatic embryos were selected on a kanamycin medium (100 mg·liter–1). The PCR analysis of the transgenic sweetpotato plants showed the presence of foreign genes in the sweetpotato genome. About 100 transgenic plants are being maintained under laboratory and greenhouse conditions. All the transgenic plants showed a strong expression of gusA gene in the histochemical GUS assay but showed quantitative differences in the chemiluminescent assay. The CaMV35S promoter shows a differential expression because there was some degree of tissue- and organ-specificity in the gusA expression. All transgenic plants appear normal with no phenotypic aberrations and are being tested for productivity traits.

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Yeh-Jin Ahn and Na-Hyun Song

expression level of DcHsp17.7, chemiluminescent signals were measured using IMAGER and 1D MAIN (Bioneer, Seoul, Korea). The values were normalized to the expression level at 1 h in leaf tissue. Heterologous expression of DcHsp17.7 in Escherichia coli. The

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Joohee Lee and Yeh-Jin Ahn

-Rad, Hercules, CA) followed by immunoblot analysis using a polyclonal antibody raised against DcHsp17.7, according to the instructions of the ECL Plus system (GE Healthcare Life Science, Buckinghamshire, U.K.). Chemiluminescent signals were detected on Hyperfilm

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Na-Hyun Song and Yeh-Jin Ahn

.7 according to the instructions of the ECL Plus system (Amersham Biosciences, Pittsburgh, PA). To quantify the expression profile of DcHsp17.7 in cold-stressed leaves, chemiluminescent signals were measured using IMAGER and 1D MAIN (Bioneer, Daejeon, Korea

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Attila Hegedűs, Emőke Balogh, Rita Engel, Béla Zoltan Sipos, János Papp, Anna Blázovics, and Éva Stefanovits-Bányai

Antioxidant defense in erythrocytes and plasma of patients with active and quiescent Crohn's disease and ulcerative colitis: A chemiluminescent study Clin. Chem. 45 895 896 Block, G. Patterson, B. Subar, A. 1992

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Hisayo Yamane, Yukinobu Kashiwa, Tomomi Ooka, Ryutaro Tao, and Keizo Yonemori

72 °C. Immunological detection of hybridized nucleic acids was carried out using the anti-DIG-alkaline phosphate conjugate and the chemiluminescent substrate CDP-star (New England Biolabs, Beverly, MA). Chemiluminescence was documented on X-ray film

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David Gopaulchan, Adrian M. Lennon, and Pathmanathan Umaharan

-Tween for 1 h at room temperature. Visualization of bound antibodies were performed with chemiluminescent substrate ( SuperSignal West Pico, Pierce Protein Research Products) according to the manufacturer’s instructions and recorded on CL-XPosure Film

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Maria A. Estrada, Kelly Zarka, Susannah Cooper, Joseph Coombs, David S. Douches, and Edward J. Grafius

DIG-labeled DNA probe. CSPD chemiluminescent detection was conducted following the manufacturer's procedures using 75 mU·mL −1 antidigoxigenin alkaline-phosphatase conjugate and 1 mL of CSPD ready-to-use substrate (Roche, Mannheim, Germany). The