-Apogee’ were studied to determine if increased RZT might confound growth responses observed in PESTO. The objectives were to determine the RZTs at which plant growth and carbon partitioning are affected as well as determine which physiological and morphologic
Oscar Monje, Sylvia Anderson, and Gary W. Stutte
Yaffa L. Grossman and Theodore M. DeJong
Plant dry matter production is proportional to light interception, but fruit production does not always increase with increased light interception. Seasonal daily patterns of light interception by cling peach trees planted in four different planting density/training systems were obtained using a Decagon ceptometer. The High Density V system (1196 trees/ha) intercepted significantly more light than the KAC V and Cordon systems (918 trees/ha). The Vase system (299 trees/ha) intercepted significantly less light than the other systems. Response surfaces using a quadratic model with interactions for time of day and day of year explained 84% to 91% of the variance in the data sets for each training system. Crop yields per acre were greatest for the High Density V, followed by KAC V, Cordon, and Vase, corresponding to the light interception data. A carbon budget model, which incorporated canopy photosynthesis, respiration, and carbon partitioning based on organ growth potentials, was used to simulate seasonal patterns of carbon assimilation, crop dry weights, and individual fruit dry weights.
Z. Wang and B. Quebedeaux
Light duration alters carbon partitioning among carbohydrates (CHOs) in source leaves. The current experiments were designed to determine how light duration affected the metabolism of newly fixed and reserve CHOs in various organs of apple and whether longer durations favored sorbitol (sor) synthesis. One-year-old `Gala' apple plants that experienced a 1-, 4-, 7-, 10-, or 14-h photoperiod for 7 d were exposed to 14CO2 for 15 min. Individual CHO concentrations and the activity of newly-fi xed [14C]CHOs in leaves, stems, and roots were analyzed during different intervals after labeling. In source leaves, sor increased significantly, whereas sucrose (suc) did not change as light duration increased from 1 to 10 h, resulting in increased sor/suc ratios from 2.6 in the 1-h to 4.3 in the 10-h light duration. The increased sor/suc ratios may be due primarily to the preferential carbon partitioning into sor in longer light durations. Longer light durations enhanced the sor export rate from source leaves, resulting in higher sor in stems and sink tissues. In roots, starch increased significantly over increasing light durations. A major portion of starch in roots may be converted from newly fixed CHO. Our results suggest that light duration alters the metabolism of sor and other CHOs in source and sink tissues of apple and that the changes in CHO concentrations result from different rates of carbon synthesis, partitioning, and export.
David C. Percival, John T.A. Proctor, and J. Alan Sullivan
Field experiments consisting of trickle irrigation (TI), IRT-76 plastic film (PF), and straw mulch were initiated to determine the influence of soil temperature and water status on carbon partitioning during the establishment of Rubus idaeus L. `Heritage' (1993, 1994), `Autumn Bliss' (1994), and `Summit' (1994) micropropagated raspberries. Environmental, vegetative, reproductive, and nutrition data were collected. Photosynthesis (Pn) measurements were recorded under field conditions using a Li-Cor LI-6200 portable photosynthesis system. Neither node number nor shoot: root ratio was influenced by TI, PF, or straw mulch. PF, however, increased root and shoot weight, total flowers produced, total berries harvested, and foliar N and P. Although differences existed among cultivars, field Pn measurements indicated that, regardless of groundcover treatment or cultivar examined, the maximum Pn rate occurred at a root-zone temperature of 25C. Hence, results from this study indicate that conditions in both the air and root zone physical environment regulate carbon assimilation and partitioning.
Justine E. Vanden Heuvel and Joan R. Davenport
Information on growth and carbon partitioning of cranberry uprights in response to soil N application is lacking. Therefore, two experiments were initiated on `Stevens' uprights to determine the effect of soil-applied N on tissue N, growth, net carbon exchange (NCER), and nonstructural carbohydrate production of uprights of `Stevens' cranberry. Tissue N concentration increased linearly with increasing soil N but was greater in vegetative uprights than in fruiting uprights. Current season growth on vegetative uprights was more responsive to tissue N than on fruiting uprights. Although chlorophyll concentration and NCER increased with increased soil N, upright starch concentration and often total nonstructural carbohydrate concentration decreased with increased soil N at midfruit development and preharvest, especially in vegetative uprights.
L. Corelli-Grappadelli, G. Ravaglia, and A. Asirelli
Carbon partitioning and translocation in 1-year-old fruiting limbs of the peach cultivar Elegant Lady was studied, as affected by source [leaves on actively growing extension shoots (EXT), or on lateral shoots emerging from nodes with (F) or without (NF) fruit], five times during the season [2, 4, 7, 11, 17 weeks after full bloom (WAFB)]. Growing shoot apices were the strongest sinks early in the season (2 WAFB), with the EXT apices capable of attracting more C, and over longer distances, than the NF or F ones. The major source of newly synthesized carbohydrates for fruit growth 2 WAFB was the F lateral shoot; i.e., the most-important C contributor for fruit growth well into stage II (11 WAFB in this study). EXT shoots began contributing to fruit growth before the NF ones: 4 WAFB on, they exported to the fruit in amounts similar to the F shoots. This contribution was even larger 17 WAFB, when the F shoots retained more C than the other two shoot types. The NF shoots exported to the growing apices 2 WAFB, but this export decreased 4, 7, and 11 WAFB, compared to the other treatments. Seventeen WAFB, they contributed to fruit growth in the same amounts as the EXT shoots, and more than the F ones. Shade did not consistently affect patterns of carbon partitioning within limb components, except for the F treatment, where it diminished C export to the fruit in the first four treatment dates. Partitioning within fruit components (mesocarp, endocarp, and seed) followed the fruit growth curve, with more C allocated to endocarp tissues at 11 WAFB (stage II).
Michelle DaCosta and Bingru Huang
Efficient carbon distribution and utilization may enhance drought survival and recovery ability for perennial grasses. The objectives of this study were to examine changes in carbon partitioning and carbohydrate accumulation patterns in shoots and roots of colonial bentgrass (Agrostis capillaris L.), creeping bentgrass (A. stolonifera L.), and velvet bentgrass (A. canina L.) in response to drought and re-watering following drought, and to determine whether species variation in drought tolerance and recuperative potential is related to differences in the patterns of carbon partitioning and accumulation. The experiment consisted of three treatments: 1) well-watered control; 2) drought, irrigation completely withheld for 18 days; and 3) drought recovery, a group of drought-stressed plants were re-watered at the end of the drought treatment (18 days). Drought tolerance and recuperative ability of three species was evaluated by measuring turf quality and leaf relative water content. These parameters indicated that velvet bentgrass was most drought tolerant while colonial bentgrass had highest recuperative ability among the three species. Plants were labeled with 14CO2 to determine carbon partitioning to shoots and roots. Carbohydrate accumulation was assessed by total nonstructural carbohydrate (TNC) content. The proportion of newly photosynthesized 14C partitioned to roots increased at 12 days of drought compared to the pre-stress level, to a greater extent for velvet bentgrass (45%) than for colonial bentgrass (35%) and creeping bentgrass (30%). In general, the proportion of 14C was highest in roots, intermediate in stems, and lowest in leaves at 12 days of drought treatment for all three bentgrass species. As drought duration and severity increased (18 days), 14C partitioning increased more in leaves and stems relative to that in roots for all three species. Stem TNC content was significantly greater for drought-stressed plants of colonial bentgrass and velvet bentgrass compared to their respective well-watered control plants, whereas no differences in stem TNC content were observed between drought-stressed and well-watered creeping bentgrass. Our results suggest that increased carbon partitioning to roots during initial drought stress represented an adaptive response of bentgrass species to short-term drought stress, and increased carbon partitioning and carbohydrate accumulation in stems during prolonged period of drought stress could be beneficial for rapid recovery of turf growth and water status upon re-watering.
Zhongchun Wang and Bruno Quebedeaux
Chlorophyll fluorescence measurements are providing insights into Photosystem II (PSII) quantum efficiency and hence are able to provide a good estimation of carbon assimilation under field conditions. A F2 generation of sibcross seedlings from a cross of `Goldspur' × `Redspur' were selected to identify genetic variations and the relationships among fluorescence parameters, carbon assimilation, and carbon partitioning in apple leaves. Mature leaves from extension shoots were analyzed for chlorophyll fluorescence with a CF-1000 chlorophyll fluorescence measurement system, photosynthetic rate with a LI-6200 portable photosynthesis system, and carbohydrates with a Shimadzu HPLC. Significant variations in leaf chlorophyll fluorescence parameters and photosynthetic rates were found. The ratio of Fv: Fm, an estimation of photochemical efficiency of PSII, decreased from ≈0.90 in June to ≈0.75 in September while the photosynthetic rates decreased from ≈8.5 in June to ≈4.5 μmol·m–2·s–l in September. The relationships between fluorescence parameters, photosynthesis, and carbohydrate partitioning were analyzed and the ratio of sorbitol to sucrose in relation to the efficiency of PSII and NADPH production will be discussed.
Caixi Zhang, Kenji Tanabe, Fumio Tamura, Akihiro Itai, and Shiping Wang
The aim of this study was to investigate the roles of spur characteristics and carbon partitioning in regulating cultivar differences in fruit size of two late-maturing japanese pear cultivars, `Atago' and `Shinkou'. The study of spur characteristics showed that the two cultivars displayed different patterns in leaf development, flower characteristics, fruit growth, and shoot type. In contrast to `Atago' with dramatically larger fruit, `Shinkou' is a heavily spurred cultivar with a higher total leaf area and leaf number per spur early in fruit growth, less vegetative shoots, and smaller fruit but larger core. No significant differences were obtained in specific leaf weight, leaf thickness, chlorophyll content, and net photosynthesis of mature leaves, and seed number per fruit between the two cultivars. The results of trace experiment with 13C revealed that on a spur basis, there were no significant differences in the amount of 13C assimilate produced by spur leaves on each labeling date except at 190 days after anthesis, however, there were highly significant differences in the amount of 13C allocated to fruit between cultivars. Moreover, a higher amount of 13C assimilates was allocated to `Atago' flesh (or fruit) than that in `Shinkou'. Analysis of relative sink strength (RSS) indicates that the sink strength of fruit was dominant over those of other organs in the spur measured in both cultivars except at the early stage of fruit growth. `Atago' exhibited a greater RSS of fruit and lower losses of 13C for respiration and export than `Shinkou'. These results suggest that the movement of photosynthates into the fruit was determined by sink strength of the fruit rather than the source strength in the two cultivars.