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Dario J. Chavez and José X. Chaparro

markers associated with traits of interest, permitting the use of marker-assisted selection (MAS) for traits expressed in late development ( Gulsen et al., 2010 ). Bulked segregant analysis (BSA) is a rapid procedure used to efficiently identify markers

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Min Wang, Wenrui Liu, Biao Jiang, Qingwu Peng, Xiaoming He, Zhaojun Liang, and Yu’e Lin

.V. 1991 Identification of markers linked to disease resistance genes by bulked segregant analysis, a rapid method to detect markers in specific genomic regions by using segregating populations Proc. Natl. Acad. Sci. USA 88 9828 9832 Murray, M. Thompson, W

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Luwbia Aranda, Timothy G. Porch, Mark J. Bassett, Laura Lara, and Perry B. Cregan

. Circumlineatus phenotype with precipitation line indicated by the arrow in representative lines from the t z cl G b v virgarcus BC 3 5-593 × t z sel Cl G b v sellatus BC 3 5-593 population of common bean. AFLP and bulk segregant analysis. DNA was

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Taifeng Zhang, Jiajun Liu, Shi Liu, Zhuo Ding, Feishi Luan, and Peng Gao

’, respectively ( Knavel, 1988 , 1990 ; Paris et al., 1984 ). Furthermore, Hwang et al. (2014) identified the dwarf gene mdw1 of the melon ‘PNU-WT1’ dwarf mutant on chromosome 7, which is highly homologous with CKX . Bulked-segregant analysis (BSA) is an

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S.O. Park, J.M. Bokosi, and D.P. Coyne

Plant growth habit is an important trait. Our objective was to identify RAPD markers linked to major gene for indeterminate growth habit using bulked segregant analysis in an F2 population from a bean cross Chichara (indeterminate growth habit × PC-50 (determinate growth habit). A total of 132 RAPD primers (600 RAPD primer screened) showed polymorphisms between bulked DNA derived from indeterminate and determinate plants. All markers showed coupling linkage with indeterminate growth habit. RAPD markers of A-8, A-17, C-7, C-15, D-4, D-5, F-6, F-16, G-9, H-3, H-20, and I-7 were 2.2 cM distant from the gene for indeterminate growth habit. Markers of B-7, B-16, B-17, C-8, E-1, F-1, F-20 and H-l9 primers were 4.6 cM distant from the gene for indeterminate growth habit.

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S.O. Park, A. Dursun, D.P. Coyne, and G. Jung

Common bacterial blight (CBB), incited by Xanthomonas campestris pv. phaseoli (Xcp), an important disease in common bean (Phaseolus vulgaris L.) Tepary bean (P. acutifolius A. Gray) is of interest to bean breeders because of resistance to CBB. Our objective was to identify RAPD markers linked to major genes for CBB resistance using bulked segregant analysis in an F2 population from a tepary bean cross CIAT640005 (R) X Nebr#4B (S). A total of 57 RAPD primers (602 RAPD primers screened) showed polymorphisms between bulked DNA derived from R and S CBB plants. All markers showed coupling linkage with CBB resistance. A good fit to a 3:1 ratio of bands for presence and absence using 11 RAPD primers was observed in 77 F2 plants. Markers of U-15 and L-7 primers were 2.4 cM distant from the gene for resistance to Xcp strain LB-2. RAPD markers of U-10, U-20, S-12, Y-4, F-13, P-6, Q-1, and Q-ll primers were 2.4 cM distant from the gene for resistance to Xcp strain SC-4A. RAPD markers of IJ-15 and L-7 primers were 8.4 cM distant from the gene for resistance to Xcp strain EKl l. The tepary RAPD linkage group includes three molecular markers and three genes for resistance to Xcp strains EK-l l, LB-2, and SC-4A and spans a length of 19.2 cM. This data supports the presence of Xcp races.

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Cunquan Yuan, Zhiyi Qu, Huitang Pan, Tangren Cheng, Jia Wang, and Qixiang Zhang

reverse primer pair sequences, simple sequence repeat (SSR) type, annealing temperature (Tm), and length of the 28 polymorphic expressed sequence tag–SSR markers that were used for bulked segregant analysis in Primula forbesii . The percentage of the

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Taifeng Zhang, Jiajun Liu, Sikandar Amanullah, Zhuo Ding, Haonan Cui, Feishi Luan, and Peng Gao

maize ( Zea mays ) and rice ( Oryza sativa ) has been reported ( Chen et al., 2014 ; Itoh et al., 2001 ; Teng et al., 2013 ). The bulked segregant analysis (BSA) is a rapid technique for mapping genes related to the measured phenotype of different

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Mónica Morales, Marisol Luís-Arteaga, José María Álvarez, Ramon Dolcet-Sanjuan, Amparo Monfort, Pere Arús, and Jordi Garcia-Mas

The recessive allele (nsv) of the NSV gene confers resistance to the Carmovirus melon necrotic spot virus (MNSV) in melon (Cucumis melo L.). Using an F2 population obtained from the cross between the resistant Korean accession PI 161375 and a susceptible line of `Piel de Sapo', we have mapped the NSV locus to linkage group 11 (G11) of the melon genome. Additional markers closely linked to NSV were developed by bulked segregant analysis (BSA) using a doubled haploid progeny population derived from the same cross. A detailed map of the NSV region was constructed containing 10 markers spanning a distance of 17.7 cM. The nearest flanking markers to NSV were two amplified fragment length polymorphisms (AFLPs) (CTA/ACG-115 and CTA/ACG-120) and one random amplified polymorphic DNA (RAPD) (OPD08-0.80) separated by 5.9 cM. Two more markers, ACC/ACC-110 and OPX15-1.06, cosegregated with NSV.

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Gino E. Beltrán, Geunwha Jung, James Nienhuis, and Mark J. Bassett

The development of a complete linkage map, including both classical (visible) and molecular markers, is important to understand the genetic relationships among different traits in common bean (Phaseolus vulgaris L.). The objective of this study was to integrate classical marker genes into previously constructed molecular linkage maps in common bean. Bulked segregant analysis was used to identify 10 random amplified polymorphic DNA (RAPD) markers linked to genes for five classical marker traits: dark green savoy leaf (dgs), blue flower (blu), silvery [Latin: argentum] green pod (arg), yellow wax pod (y) and flat pod (a spontaneous mutation from round to flat pod in `Hialeah' snap bean). The genes for dark green savoy leaf (dgs) and blue flower (blu) were located in a previously constructed molecular linkage map. These results indicate that classical marker genes and molecular markers can be integrated to form a more complete and informative genetic linkage map. Most of the RAPD markers were not polymorphic in the two mapping populations used, and molecular markers from those mapping populations were not polymorphic in the F2 populations used to develop the RAPD markers. Alternative genetic hypotheses for the pod shape mutation in `Hialeah' are discussed, and the experimental difficulties of pod shape classification are described.