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David M. Czarnecki II, Amanda J. Hershberger, Carol D. Robacker, David G. Clark and Zhanao Deng

and pollen stainability of 32 L . camara cultivars and breeding lines. The main objective was to gain a better understanding of the relationship between ploidy level and pollen stainability in L . camara and to assess the effectiveness of ploidy

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Narinder P.S. Dhillon, Supannika Sanguansil, Supornpun Srimat, Roland Schafleitner, B. Manjunath, Parag Agarwal, Qu Xiang, Mohammed Abu Taher Masud, Thaingi Myint, Ngo Thi Hanh, Tran Kim Cuong, Conrado H. Balatero, Venus Salutan-Bautista, Michel Pitrat, Aleš Lebeda and James D. McCreight

; Kousik et al., 2011 ; Mercier et al., 2014 ; Zhang et al., 2011 ). Furthermore, we evaluated horticultural fruit characters of the CPM-resistant bitter gourd breeding lines in the field test at Kamphaeng Saen, Thailand in 2014, and assessed the yield

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Christian A. Wyenandt, James E. Simon, Margaret T. McGrath and Daniel L. Ward

basil downy mildew in 2009. The objective of this study was to evaluate current commercial cultivars, sweet basil breeding lines, and other Ocimum spp. for their potential susceptibility to basil downy mildew. Materials and Methods In

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Pheonah Nabukalu and Carrie A. Knott

its potential as an ornamental grass, saturation-tolerant sea oats lines are needed. In this study, we examined initial strategies toward testing, identifying, and developing saturation-tolerant sea oats breeding lines for use in beach restoration and

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Samuel F. Hutton, Jay W. Scott and Jeffrey B. Jones

. Three advanced breeding lines have been developed that have non-hypersensitive resistance to race T4. Fla. 8233 is a large-fruited fresh-market tomato with PI 128216 and H7998 in its pedigree; it has a moderate to high level of resistance to race T4. Fla

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Monica Ozores-Hampton, Philip A. Stansly and Eugene McAvoy

recommendations including new varieties and breeding lines previously not evaluated under low and high TYLCV pressure. Relative to susceptible varieties, such as Florida 47, Sebring (round), and Mariana (Roma-type), TYLCV-R varieties evaluated produced comparable

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Sergey Nesterenko and Kenneth C. Sink

Lutein and zeaxanthin are becoming established as carotenoids beneficial for protection against common age-associated eye diseases. Thus, 15 potato (Solanum tuberosum subsp. tuberosum L.) breeding lines, cultivars Atlantic, Spunta, and Yukon Gold; and orange flesh OR-4 were surveyed for carotenoid profiles. Seven carotenoids, including violaxanthin, neoaxanthin, antheraxanthin, lutein, zeaxanthin, β-cryptoaxanthin, and β-carotene, were identified in the 19 genotypes. Violaxanthin and lutein were the prominent carotenoids detected in all genotypes studied. Neoaxanthin and antheraxanthin were found in 26% and 63% of the genotypes, respectively. β-Cryptoaxanthin, zeaxanthin, and β-carotene were found in only 5%, 10%, and 16% of the genotypes, respectively. Lutein varied from 19.8 to 119.0 μg·100 g-1 fresh weight across the 15 white- or yellow-flesh breeding lines. In contrast, zeaxanthin was detected at a low level in only one breeding line and at high level in OR-4. The three cultivars had profiles typical of yellow-flesh potatoes `Spunta' and `Yukon Gold'; while `Atlantic' had a typical white-flesh profile and a trace of zeaxanthin. The carotenoid baseline data established in this study provide information for activities to enhance potato for lutein and zeaxanthin.

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Marisa Maiero, Timothy J Ng and Thomas H. Barksdale

Tomato (Lycopersicon esculentum Mill.) genotypes evaluated for early blight [Alternaria solani (Ellis & Martin) Jones and Grout] resistance included five tolerant breeding lines, a susceptible cultivar, and seven hybrids among them. Three of the genotypes (`Castlejay', NC EBR-2, and 87B187) were crossed in a diallel mating design to estimate general combining ability and specific combining ability for the resistance trait. Parental, F1, F2, and backcross generations of the family Cl943 x `Castlejay' were evaluated for resistance and included in generation mean analysis. Hybrid means for area under the disease progress curve were not significantly different from respective midparent values, indicating additive genetic control. Diallel and generation mean analyses also detected significant additive genetic effects. Epistasis was present in the Cl943 × `Castlejay' family.

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Ryan J. Hayes, Carolee T. Bull, Polly H. Goldman and Edward J. Ryder

Bacterial leaf spot of lettuce caused by Xanthomonas campestris pv. vitians (Xcv) is an important lettuce disease in California. No adequate control measures have been found, although resistance exists in several heirloom cultivars. Deployment of cultivars resistant to bacterial leaf spot will reduce these periodic and costly disease events. The objectives of this research were to 1) identify new sources of resistance within modern crisphead cultivars and 2) select for resistance in `Salad Crisp' × `Iceberg' progeny. Field plots were established and grown with overhead irrigation, and a three-strain mixture of Xcv was applied until runoff 1 week after thinning at 1 × 109 CFU/mL. Twenty-six crisphead cultivars were tested in unreplicated field trials and rated on a 1 (susceptible) to 4 (resistant) scale. Selection was carried out between and within families from the F2 to F4 generation. Sixteen F3 families were evaluated in unreplicated plots, and 12 F5 families were tested in replicated plots for disease incidence and severity. No usable levels of resistance were identified in the modern crisphead cultivars tested to date. All F3 families had resistance greater than `Iceberg', and 19 plants from eight families were selected for further breeding. Subsequently, 12 plants from two F4 families were selected. Replicated trials of 12 F5 families indicated that all lines have disease severity comparable to both parents. Breeding lines from crosses to `Salinas 88' are currently being developed.

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Ana Fita, Belén Picó, Rita C.S. Dias and Fernando Nuez

) resistant breeding lines. Each cross stage was followed by a selection for root architecture and resistance to melon vine decline; a 5% to 10% selection pressure was applied. Fig. 2. Roots (from plants grown for 90 d in 18-L pots filled with