Bottle gourd [ Lagenaria siceraria (Molina) Standl.] belongs to the Cucurbitaceae family. It is a diploid (2 n = 2 x = 22) vine crop widely grown in rural communities in South Africa ( Achigan-Dako et al., 2008 ; Beevy and Kuriachan, 1996 ). The
Jacob Mashilo, Hussein Shimelis, Alfred Odindo, and Beyene Amelework
Matthew A. Cutulle, Howard F. Harrison Jr., Chandresakar S. Kousik, Phillip A. Wadl, and Amnon Levi
jars, utensils, tubes, and musical instruments, is used as a vegetable, and is commonly known as the bottle gourd ( Decker-Walters et al., 2001 , 2004 ; Erickson et al., 2005 ). It is also known as the white-flowered gourd or calabash ( Jeffrey, 1967
Asma Mohammed Saeed Al-Kubati, Baoshan Kang, Liming Liu, Aqleem Abbas, and Qinsheng Gu
cropping ( Colla et al., 2010 ). For cucurbits, the bottle gourd ( Lagenaria siceraria ) is an essential rootstock used to improve the disease resistance of grafted plants (also known as a scion), especially watermelon or melon ( Gaion et al., 2017
Chandrasekar S. Kousik, Amnon Levi, Kai-Shu Ling, and W. Patrick Wechter
Bottle gourd [ Lagenaria siceraria (Mol.) Standl.], which belongs to the Cucurbitaceae family, is considered to be one of the earliest species of plants to be domesticated by humans. Bottle gourds are regularly grown and consumed in various parts
Shawna L. Daley and Richard L. Hassell
commonly used in Asia and Europe, bottle gourd ( Lagenaria sicereria cv. Emphasis) (Syngenta Seeds, Boise, ID) and interspecific hybrid squash ( Cucurbita maxima × Cucurbita moschata cv. Carnivor) (Syngenta Seeds), were sown in 72-cell, TLC polyform
Jeung-Sul Han* and Chang Kil Kim
A procedure for producing transgenic bottle gourd plants by inoculating cotyledon explants with Agrobacterium tumefaciens strain AGL1 carrying a binary vector pCAMBIA3301, which contains glufosinate ammonium-resistant (bar) and the reporter (gus) genes, is describe. Infection was the most effective (highest infection frequency and index) when explants were co-cultivated with Agrobacterium for 6-8 days on co-cultivation medium supplemented with 0.001-0.1 mg/L L-a-(2-aminoethoxyvinyl) glycine (AVG). Transgenic plants were obtained with frequencies of about 0.2% when the explants were cultured on selection medium (MS medium supplemented with 3.0 mg/L BAP, 0.5 mg/L AgNO3, 500 mg/L cefotaxime, 2.0 mg/L DL-phosphinothricin, 0.3% sucrose and 0.8% Plant Agar. A histochemical gus assay, PCR and Southern blot analyses confirmed that transformation had occurred. Genetic analysis of T1 progenies showed that the transgenes were inherited in a Mendelian fashion. To our knowlege, this study represents the first report for Agrobacterium-mediated transformation in bottle gourd, rootstock for watermelon and other cucurbit crops in many countries.
Chandrasekar S. Kousik, Jennifer L. Ikerd, Mihir K. Mandal, Scott Adkins, Craig G. Webster, and William W. Turechek
USVL351-PMR (powdery mildew resistant) and USVL482-PMR are bottle gourd ( Lagenaria siceraria ) germplasm lines that exhibit high levels of resistance to the cucurbit powdery mildew (PM) pathogen ( Podosphaera xanthii ) compared with susceptible
Seung-Hee Lee*, Jin-Seok Kim, and Jung-Myung Lee
Dry heat treatment (DHT), a powerful and agrochemical-free means of inactivating seed-borne virus and other pathogens, has been extensively used for value-added vegetable seeds in Korea, Japan, and some other countries. Since seeds are treated with extremely high heat (75 °C or higher) for a long time (72 h or longer), heat-induced phytotoxicity symptoms are frequently observed. Even though various internal and external factors, such as seed maturity and vigor, maximum temperature and duration of DHT, are known to influence the severity of phytotoxicity, precise control of seed moisture contents during DHT is regarded as one of the most important factors for successful DHT. In an ideal condition using a specifically designed DTH machine, seed moisture content of bottle gourd, initially around 6.20% to 0.64% when stored in a storage room with 50% RH, decreased by 1% after 24 h at 35 °C (5.20% to 0.23%), and further decreased below 4% after 24 h pretreatment at 50 °C (3.64% to 0.37%). The seed moisture content was further reduced down to about 2% after 72 h DHT at 75 °C (2.16% to 0.28%). During the post-treatment conditioning at 50 °C and 70% RH for 24 h, the moisture contents were raised to about 6%(5.94% to 0.45%), thus approaching the initial moisture content of 6% to 7%. During the germination period, treated seeds showed slower absorption of water as compared to the intact seeds, thus suggesting that this slow absorption of initial moisture absorption may be responsible for the slow initial germination frequently observed in treated seeds. Final germination and seedling vigor were not affected by DHT.
Shawna L. Daley, Jeffrey Adelberg, and Richard L. Hassell
a starch increase of 100- and 200-fold in hypocotyls of bottle gourd and interspecific hybrid squash rootstocks, respectively, over 21 d after fatty alcohol treatment ( Daley et al., 2014 ). We hypothesize that this increase of stored energy in the
G. F. Iapichino and J. B. Loy
The effect of high temperature on pollen viability and fruit set was studied in a Sicilian landrace of Lagenaria siceraria (Mol.) Standi. In vitro germination of pollen from flowers of plants exposed for 7 hr to either 28° or 33°C was not significantly different from that of pollen from untreated, greenhouse-grown plants. A single 7-hr exposure of plants to 38° completely inhibited germination of pollen from flowers reaching anthesis either immediately following the treatment or 24 hr later, and substantially reduced germination of pollen from flowers reaching anthesis at 48 or 72 hr after treatment. Pollen from flowers subjected to 38° for 7 hr failed to germinate and grow into styles of untreated female flowers and failed to induce fruit set. Exposure of plants to 38° for 4 hr reduced in vitro pollen germination by 55% to 75%, but a 2-hr treatment at 38° had no detectable effect on pollen viability.