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Genyi Li and Carlos F. Quiros

DNA samples from 21 cultivars of celery (Apium graveolens L. var. dulce) were subjected to amplified fragment length polymorphism (AFLP) analysis. The most informative adapter combination was EcoRI-TaqI. All cultivars could be distinguished from each other by their unique fingerprints based on 73 markers. The program NTSYS grouped the cultivars in three main clusters according to their origin. The groupings observed agreed, with a few exceptions, with those expected by historical accounts and previous analyses based on biochemical and ramdomly amplified polymorphic DNA (RAPD) markers.

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Marie-José Côté and Lisa Leduc

requests for cultivar verification after morphologic examination were also included to expand the analysis. Table 1. Japanese barberry and holly varieties used to generate reference amplified fragment length polymorphism patterns. Table 2

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Min Deng, Jianjun Chen, Richard J. Henny, and Qiansheng Li

relationships among cultivars have not been established ( Deng et al., 2010 ; Sharma and Bal., 1958 ), which has hampered our efforts on croton germplasm conservation and new cultivar development. Amplified fragment length polymorphism (AFLP), a polymerase

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Ryan N. Contreras, Thomas G. Ranney, Susana R. Milla-Lewis, and G. Craig Yencho

relationships among plants. The amplified fragment length polymorphism (AFLP) technique ( Vos et al., 1995 ; Zabeau and Vos, 1993 ), in particular, has been used by many scientists to distinguish between species as well as cultivars of the same species ( DeHaan

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Yeun-Kyung Chang, Richard E. Veilleux, and Muhammad Javed Iqbal

and unlimited in number ( Agarwal et al., 2008 ). Several types of molecular markers are available; however, amplified fragment length polymorphism (AFLP) markers have notable advantages such as reproducibility, high levels of polymorphism that can

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Qingguo Ma, Junpei Zhang, and Dong Pei

Province, Shandong Province, Xinjiang Province, and Yunnan Province ( Table 1 ; Fig. 1 )], then frozen quickly and stored at –80 °C until DNA extraction. Table 1. Accessions chosen for the amplified fragment length polymorphism analysis of walnut cultivars

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Jennifer A. Kimball, M. Carolina Zuleta, Matthew C. Martin, Kevin E. Kenworthy, Ambika Chandra, and Susana R. Milla-Lewis

. augustinegrass ‘Raleigh’ and ‘Palmetto’ samples assessed for genetic variability using 143 polymorphic amplified fragment length polymorphism markers and their genetic similarity values with original stocks of ‘Raleigh’. For DNA extraction, four young unopened

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Deborah Pagliaccia, Georgios Vidalakis, Greg W. Douhan, Ramiro Lobo, and Gary Tanizaki

-μL volume of low-salt buffer AE. The quality and quantity was evaluated in 0.8% agarose gels stained with SYBR Green I (Molecular Probes, Eugene, OR). Amplified fragment length polymorphisms. The method of Vos et al. (1995) was used to develop the

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Jinggui Fang, Jianjun Chen, Richard J. Henny, and Chih-Cheng T. Chao

marker techniques, amplified fragment length polymorphism (AFLP) is a novel polymerase chain reaction (PC)R-based assay for DNA fingerprinting and polymorphism detection ( Vos et al., 1995 ). The advantages of this technique include reproducibility, high

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Ambika B. Gaikwad, Tusar Kanti Behera, Anand K. Singh, Devanshi Chandel, Jawahir L. Karihaloo, and Jack E. Staub

, however, could not give complete insight into the cultigens examined. The discriminatory power of amplified fragment length polymorphism (AFLP) is often found to be greater than that of RAPD and ISSR markers ( Powell et al., 1996 ; Vos et al., 1995 ) as a