Search Results

You are looking at 1 - 10 of 13 items for :

  • "allele identification" x
  • Refine by Access: All x
Clear All
Free access

Rafel Socias i Company, Àngel Fernández i Martí, Ossama Kodad, and José M. Alonso

( Bošković et al., 2003 ). Allele Identification The more recent advances in genetic analysis at the gene level have allowed a closer approach to the S f allele in almond both of the stylar and the pollen components. First, S alleles, including S

Free access

Shogo Matsumoto and Kentaro Kitahara

A polymerase chain reaction (PCR)-based method for identifying the S-alleles in the Asian pear [Pyrus pyrifolia (Burm) Nak.] was applied to apple (Malus ×domestica Borkh.) cultivars. With minor modifications in one of the primers, the fragments from S-genes (S-RNases) with introns were amplified from total DNA of apple cultivars possessing S2-, S3-, S5-, S7-(=Sd-), S9-(=Sc-), Sf- and Sg-allele genotypes. S-genes within S24-(=Sh-) and S26-alleles were also amplified. The PCR amplification step of this method appears to be useful for preliminary investigation of apple S-genotypes, especially for species or cultivars of unknown origin or history. Using the primers, which are a part of a new S-allele, the Se-allele encoding Se-RNase with an intron in the Se-allele was amplified. We cloned the cDNA of Se-RNase, and developed a PCR-restriction fragment length polymorphism (RFLP) analysis method for Se-allele identification. S-allele genotypes of seven apple cultivars were investigated.

Open access

Melinda A. Miller-Butler, Barbara J. Smith, Brian R. Kreiser, and Eugene K. Blythe

isolates as C. nymphaeae , and the C. gloeosporioides isolate as C. fructicola ( Miller-Butler et al., 2018 ; Munir et al., 2016 ). DNA Extractions and Allele Identification. The same collection of plants used in the study by Miller-Butler et al

Open access

Alfonso Guevara, María Nicolás-Almansa, José Enrique Cos, Juan Alfonso Salazar, Domingo López, José Egea, Antonio Carrillo, Manuel Rubio, Federico García, and David Ruiz

. Rodrigo, J. 2020 Self-(in)compatibility, S-RNase allele identification, and selection of pollinizers in new Japanese plum-type cultivars Scientia Hort. 261 109022 10.1016/j.scienta.2019.109022 Guerra, M.E. Rodrigo, J. López-Corrales, M

Free access

Keiko Sekido, Yusaku Hayashi, Kunio Yamada, Katsuhiro Shiratake, Shogo Matsumoto, Tsutomu Maejima, and Hiromitsu Komatsu

427 Kitahara, K. Matsumoto, S. 2002a Cloning of the S 25 cDNA from ‘McIntosh’ apple and an S 25 -allele identification method J. Hort. Sci. Biotechnol. 77 724 728 Kitahara, K. Matsumoto

Free access

Hitomi Umemura, Katsuhiro Shiratake, Shogo Matsumoto, Tsutomu Maejima, and Hiromitsu Komatsu

of the S 25 cDNA from ‘McIntosh’ apple and an S 25 -allele identification method J. Hort. Sci. Biotechnol. 77 724 728 Kitahara, K. Matsumoto, S. 2002b Sequence of the S 10 cDNA from ‘McIntosh’ apple and a PCR-digestion identification method

Free access

Gayle M. Volk, Adam D. Henk, Christopher M. Richards, Philip L. Forsline, and C. Thomas Chao

chain reaction amplifications, and allele identifications have been described previously ( Volk et al., 2005 ). Two sets of data were available. A total of 18 simple sequence repeat (SSR) loci (GD12, GD15, GD96, GD142, GD147, GD162, CH01h01, CH01f02, CH

Free access

Jung Hyun Kwon, Ji Hae Jun, Eun Young Nam, Kyeong Ho Chung, Ik Koo Yoon, Seok Kyu Yun, and Sung Jong Kim

.J. Broekaert, W.F. Broothaerts, W. 1995 A molecular method for S -allele identification in apple based on allele-specific PCR Theor. Appl. Genet. 91 691 698 Jun, J.H. Kwon, J.H. Chung, K.H. 2007 Identification of self-incompatibility genotypes in Japanese plum

Free access

R.L. Rusholme Pilcher, J-M. Celton, S.E. Gardiner, and D.S. Tustin

screened similarly over the subset of 30 individuals, as well as DNA from ‘Fiesta’ that was included as a positive control and to aid in allele identification. For the RAPD analysis, the reaction mixture and amplification program used were as described by

Free access

Javier Sanzol and Timothy P. Robbins

, W. 1995 A molecular method for S-allele identification in apple based on allele-specific PCR Theor. Appl. Genet. 91 691 698 Kester, D.E. Gradziel, T.M. Micke, W.C. 1994 Identifying pollen