Search Results

You are looking at 1 - 7 of 7 items for :

  • "agar concentration" x
Clear All
Restricted access

Georgia Vlachou, Maria Papafotiou and Konstantinos F. Bertsouklis

of storage and temperature on C. nepeta seed germination ability, and 2) the effect of various plant growth regulators and agar concentrations on in vitro shoot proliferation, elimination of hyperhydricity problems, and microshoot rooting. Materials

Free access

Xiaoling Yu and Barbara M. Reed

Multiplication and elongation of shoot cultures established from mature trees of hazelnut cvs. Nonpareil and Tonda Gentile Romana were affected by changes in basal medium, carbon source and concentration, cytokinin and agar concentration. Explants on DKW medium produced significantly more shoots than those on Anderson medium or modified woody plant medium for chestnut. Explants on DKW medium with 3% glucose or fructose gave more and longer shoots than those with the other carbon sources. Cytokinins 6 benzylaminopurine (BA) and zeatin were more effective in producing shoots than kinetin and 2iP. On BA supplemented medium, the best multiplication rate was obtained with 1.5 - 2.0 mg/l. Explants grown on 0.4% agar produced more shoots than those on 0.6%, however, prolonged culture on 0.4% agar caused vitrification of lower parts of the plants. Shoot multiplication rates of these two cultivars were similar, but `Nonpareil' produced longer shoots than `Tonda Gentile Romana'.

Free access

Luiz A. Biasi and Patrick J. Conner

media was placed in each well. Only in the trial of agar concentrations, to evaluate the treatment without agar, cellophane booklets were used inside petri dishes ( Alexander and Ganeshan, 1989 ). These booklets consisted of seven layers of filter paper

Restricted access

Xiaoxin Shi, Lili Yang, Guijun Yan and Guoqiang Du

production of metabolism-enhancing phytoecdysteroids from Ajuga turkestanica Plant Cell Tissue Organ Cult. 93 73 83 Cui, G. Liu, Y. Gu, Y. 2004 Effects of agar concentration, pH value and inoculated shoot size on propagation of Oncidium test-tube plantlet

Full access

Danny L. Barney, Omar A. Lopez and Elizabeth King

-to-chloride ratio of 5.2:1. The high salt concentrations may also have interfered with gelling of the agar in FSMS, which required 8 g·L −1 of agar to gel compared with 7 g·L −1 for the other media. The higher agar concentration in FSMS was unlikely to have

Free access

Ayse Tascan, Jeff Adelberg, Mevlut Tascan, Agnes Rimando, Nirmal Joshee and Anand K. Yadav

). Hyperhydricity may arise from high humidity, low light intensity, high concentration of plant growth regulators, and tissue wounding. Also, differences in support material such as reduced agar concentration (from 0.8% to 0.58%; Saher et al., 2004 ), switching

Free access

María del Carmen Vadillo-Pro, Luis Hernández-Sandoval, Guadalupe Malda-Barrera, María Luisa Osorio-Rosales and Martín Mata-Rosas

physiologic disorder can be controlled or reduced by increasing the agar concentration, which reduces the relative humidity and osmotic potential of the culture medium, but may also decrease shoot formation (George et al., 2008; Ivanova and Van Staden, 2011