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Susana Boso Alonso, Virginia Alonso-Villaverde Pilar Gago, José L. Santiago, Mariá C. Martínez, and Emilio Rodriguez

The grapevine cultivar Albariño ( Vitis vinifera L.) has long been grown in northwestern Spain and the north of Portugal. The approval of the Appellation Contrôlée (A.C) “Rías Baixas” denomination in northwestern Spain in 1988 ( Ministerio de

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Michael E. Tarter and Stefano Poni

The variate “cluster weight” is an important Vitis vinifera vine yield component and its main subcomponents are berry number and berry weight ( Clingeleffer et al., 2000 ). This variate's values can be affected by factors that include scion

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Cassandra M. Plank, Edward W. Hellman, and Thayne Montague

MPs are a class of aroma compounds responsible for vegetal aromas in fruit of some Vitis vinifera L. wine grape cultivars. MPs have been quantified in Grenache ( López et al., 1999 ), Merlot ( Sala et al., 2000 ), Cabernet Franc ( Allen et al

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Fucheng Shan and Kevin Seaton

propagation techniques. Materials and Method Plant materials and preparation All the plant propagation materials were maintained in pots in a greenhouse at the Department of Agriculture and Food, Western Australia, South Perth. The grapevines ( Vitis vinifera

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Danijela Janjanin, Marko Karoglan, Mirjana Herak Ćustić, Marijan Bubola, Mirela Osrečak, and Igor Palčić

Austral. J. Grape Wine Res. 11 242 295 Bell, S.-J. Robson, A. 1999 Effect of nitrogen fertilization on growth, canopy density, and yield of Vitis vinifera L. cv. Cabernet Sauvignon Amer. J. Enol. Viticult. 50 351 358 Bergmeyer, H.U. Beutler, H.-O. 1990

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Wanmei Jin, Jing Dong, Yuanlei Hu, Zhongping Lin, Xuefeng Xu, and Zhenhai Han

a widely grown fruit crop in the world. ‘Centennial Seedless’ of Vitis vinifera L. is one of the most widely grown grape varieties in China because it is suitable for both table consumption and wine production ( Kong, 2004 ). The berries of the

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Letizia Tozzini, Paolo Sabbatini, and G. Stanley Howell

during the ripening period in Vitis vinifera L Planta 192 567 573 Candolfi-Vasconcelos, M.C. Koblet, W. Howell, G.S. Zweifel, W. 1994b Influence of defoliation, rootstock, training system, and leaf position on gas exchange of Pinot noir grapevines Amer

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D.W. Ramming, R.L. Emershad, P. Spiegel-Roy, N. Sahar, and I. Baron

Immature grape embryos from early ripening genotypes of Vitis vinifera were successfully cultured in vitro on Difco orchid agar or a modified White's agar medium. Germination was increased in vitro for five genotypes from 0%, 7%, 11%, 12%, and 16% in vivo to 15%, 24%, 23%, 34%, and 24%, respectively. Subculturing embryos onto liquid culture from seeds that failed to germinate on agar also was possible. Differences in germination rates, as affected by pollen, were significant. This method will allow accelerated development of early ripening cultivars by allowing breeders to use such genotypes as females, as well as males.

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F.J. Montero, J.A. de Juan, A. Cuesta, and A. Brasa

The importance of rapid, nondestructive, and accurate measurements of leaf area (LA) in agronomic and physiological studies is well known, but a search of the literature revealed little information available for grape (Vitis vinifera L.). The results described herein include a comparison of 12 different mathematical models for estimating leaf area in `Cencibel'. The simplest, most accurate regression equations were: LAi = 0.587 LW (R 2 = 0.987) and LAi = 0.588 LW (R 2 = 0.994), where LAi is leaf area measured using image analysis and LW is leaf length × maximum width. Use of maximum width (W), leaf length (L), petiole length (Lp), and dry weight of leaves (DML) as single variables in the regression equations were not as closely associated with total leaf area, although their R 2 values were also highly significant.

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R. Scorza, J.M. Cordts, D.J. Gray, D. Gonsalves, R.L. Emershad, and D.W. Ramming

Transgenic grape plants were regenerated from somatic embryos derived from leaves of in vitro-grown plants of `Thompson Seedless' grape (Vitis vinifera L.) plants. Somatic embryos were either exposed directly to engineered Agrobacterium tumefaciens or they were bombarded twice with 1-μm gold particles and then exposed to A. tumefaciens. Somatic embryos were transformed with either the lytic peptide Shiva-1 gene or the tomato ringspot virus (TomRSV) coat protein (CP) gene. After cocultivation, secondary embryos proliferated on Emershad/Ramming proliferation (ERP) medium for 6 weeks before selection on ERP medium containing 40 μg·mL-1 kanamycin (kan). Transgenic embryos were identified after 3 to 5 months under selection and allowed to germinate and develop into rooted plants on woody plant medium containing 1 μm 6-benzylaminopurine, 1.5% sucrose, 0.3% activated charcoal, and 0.75% agar. Integration of the foreign genes into these grapevines was verified by growth in the presence of kanamycin (kan), positive β-glucuronidase (GUS) and polymerase chain-reaction (PCR) assays, and Southern analysis.