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Santiago García-Martínez, Adrián Grau, Aranzazu Alonso, Pedro Carbonell, Juan F. Salinas, José A. Cabrera, and Juan J. Ruiz

(Seminis Vegetable Seeds, Saint Louis, MO). ‘Anastasia F 1 ’ was used as the donor parent of the Tm-2a , Sw-5 , and Ty-1 genes ( Pérez de Castro et al., 2007 ), conferring resistance to ToMV, TSWV, and TYLCV, respectively. Twelve generations of

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Santiago García-Martínez, Adrián Grau, Aranzazu Alonso, Pedro Carbonell, Juan Francisco Salinas, José Ángel Cabrera, and Juan J. Ruiz

parent of the Tm-2a, Sw-5, and Ty-1 genes ( Pérez de Castro et al., 2007 ), conferring resistance to ToMV, TSWV, and TYLCV, respectively. Six generations of backcrosses were performed to the ‘Pera294’ cultivar using Cleaved Amplified Polymorfhic

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Yuanfu Ji and John W. Scott

Resistance to begomoviruses tomato mottle virus (ToMoV) and tomato yellow leaf curl virus (TYLCV) has been introgressed to tomato (Lycopersicon esculentum) from L. chilense accessions LA 1932, LA 2779, and LA 1938. Resistance genes have been mapped to three regions on chromosome 6 using randomly amplified polymorphic DNA (RAPD) markers. We call these regions 1, 2, and 3. To facilitate breeding by marker assisted selection, advanced breeding lines with resistance from the above sources were assayed for the presence of RAPD markers to determine which were most tightly linked to begomovirus resistance. The best RAPD markers were then converted to sequence characterized amplified region (SCAR) markers or cleaved amplified polymorphic sequence (CAPS) markers. In addition, selected restriction fragment length polymorphism (RFLP) markers near the three regions were converted into CAPS markers, which were tested for association with the advanced breeding lines. Only LA 2779 derivatives have the L. chilense introgression in region 1, which is near the location of the Ty-1 gene and spans across CAPS markers 32.5Cla and TG118. Two region 1 RAPD markers UBC197 and UBC621 were converted co-dominant SCAR or CAPS markers, which were present in all 16 resistant breeding lines tested. Derivatives from all three accessions have introgressions in region 2. Further assays with more markers in this region are under way to determine the lengths and locations of the introgressions. No tightly linked RAPD markers have been found for the resistance gene from LA 1932 in region 3. RFLP and CAPS markers are being used to more precisely locate the region 3 gene.

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Santiago García-Martínez, Adrián Grau, Aranzazu Alonso, Fernando Rubio, Manuel Valero, and Juan J. Ruiz

the donor parent of the Tm-2 a , Sw-5, and Ty-1 genes ( Pérez de Castro et al., 2005 ), conferring resistance to ToMV, TSWV, and TYLCV, respectively. ‘Anastasia’ is a popular tomato cultivar in Spain with indeterminate and vigorous plant growth as

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Samuel F. Hutton, John W. Scott, and Joshua H. Freeman

‘Fla. 8970’ is a fresh market hybrid tomato ( Solanum lycopersicum ) that has resistance to Tomato yellow leaf curl virus (TYLCV) conferred by the Ty-1 gene ( Verlaan et al., 2013 ) and to fusarium crown and root rot (FCR) incited by Fusarium

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John W. Scott, Samuel F. Hutton, and Joshua H. Freeman

curl virus (TYLCV) are among the most destructive, the most widespread, and the most well known. TYLCV-resistant cultivars are commercially available in many production regions, and most of these use the dominant Ty-1 gene. Generally, tomato growers

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Santiago García-Martínez, Adrián Grau, Aranzazu Alonso, Fernando Rubio, Pedro Carbonell, and Juan J. Ruiz

for fruit morphological characteristics, uniformity, and high yield) with the commercial cultivar Anastasia F 1 (Seminis Vegetable Seeds, Saint Louis, MO), used as the donor parent of the Tm-2 a , Sw-5 , and Ty-1 genes ( Pérez de Castro et al

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Samuel F. Hutton and John W. Scott

sweetpotato whitefly ( Bemisia tabaci ), the strains that cause Tomato yellow leaf curl virus (TYLCV) are the most widespread and well known. Begomovirus resistance in Fla. 7907C is conferred by the Ty-1 gene, which was advanced using marker

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Santiago García-Martínez, Adrián Grau, Aranzazu Alonso, Fernando Rubio, Manuel Valero, and Juan J. Ruiz

tomato ( Rubio et al., 2012 ), although the yield problems are mainly the result of the introgression of the Ty-1 gene for Tomato yellow curl virus (TYLCV) resistance. For this reason, we have developed the breeding lines UMH 1422 and UMH 1415 without

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Santiago García-Martínez, Adrián Grau, Aranzazu Alonso, Fernando Rubio, Manuel Valero, and Juan J. Ruiz

’ line. Anastasia was used as the donor parent of the Tm-2 a , Sw-5, and Ty-1 genes ( Pérez de Castro et al., 2007 ), conferring resistance to ToMV, TSWV, and TYLCV, respectively. At the time the breeding program began, ‘Anastasia’ was a popular