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Etienne L. LeRiche, Gefu Wang-Pruski, and Valtcho D. Zheljazkov

. 2007 The effect of different N and K sources on tuber nutrient uptake, total and graded yield of potatoes ( Solanum tuberosum L.) for processing Eur. J. Agron. 26 187 197 Hughes, J.C. 1962 Chemistry of after

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N.C. Yorio, R.M. Wheeler, and R.C. Weigel

Growth measurements of potato (Solanum tuberosum L.) cvs. Norland (NL), Denali (DN), and Kennebec (KN) were taken from 21-day-old plantlets grown in vitro. Studies were conducted in a growth chamber, with nodal explants grown in culture tubes with loose-fitted Magenta 2-way caps containing Murashige and Skoog salts with either 0, 1, 2 or 3% sucrose. The cultures received either 100 or 300 μmol m-2 s-1 photosynthetic photon flux (PPF), and the growth chamber was maintained at either 400 or 4000 μmol mol-1 CO2. All cvs. showed significant increases in growth on 0% sucrose media at 4000 μmol mol-1 CO2, indicating an autotrophic response. At 400 μmol mol-1 CO2, all cvs. showed an increase in total plantlet dry weight (DW) with increasing sucrose under both PPF levels. Within any sucrose treatment, the highest total DW for all cvs. resulted from 300 μmol m-2 s-1 PPF and 4000 μmol mol-1 CO2. At 4000 μmol mol-1 CO2, shoot DW declined with sucrose above 2% for DN and sucrose above 1% for NL at both PPF levels, suggesting that high sucrose levels may hinder growth when CO2 enrichment is used.

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Servet Kefi, Paul E. Read, Alexander Pavlista, and Stephen D. Kachman

To determine the influence of gibberellic acid (GA3) and 6-furfuryl aminopurine (kinetin) concentrations alone and in combinations on in vitro tuberization of potato, nine treatments consisting of combinations of gibberellic acid and kinetin at three levels of concentration (0, 2, and 5 mg·liter–1) were included in Murashige and Skoog medium supplemented with 6% sucrose. Four single nodes of in vitro plantlets from Solanum tuberosum L. cultivar Atlantic were placed into each magenta box. All magenta boxes were arranged in a randomized complete box design with five replications and cultured under a short photoperiod condition (8 h light/16 h dark). Gibberellic acid strongly inhibited tuberization when used alone or with kinetin, whereas kinetin induced tuberization at both 2 and 5 mg·liter–1. Although tuberization was initiated in the absence of kinetin because of the high concentration of sucrose and short photoperiod, the presence of kinetin accelerated the in vitro tuberization process of potato.

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Xuan-Chun Piao, Debasis Chakrabarty, Eun-Joo Hahn, and Kee-Yoeup Paek

In vitro nodal cuttings of potato (Solanum tuberosum L.) `Atlantic' and `Russet Burbank' from bioreactor culture were hydroponically cultured for 28 days using a deep flow technique (DFT) system. The response of plant growth and photosynthesis to different levels of solution electrical conductivity (EC; 0.08, 0.15, 0.22 and 0.36 S·cm-1) and pH (3, 4, 5, 6 and 7) were studied. The best growth, characters of shoot length, total shoot and root fresh and dry weight, were obtained in nutrient solution of pH 6.0 and EC 0.15 S·cm-1 for `Atlantic', while pH 7.0 and EC 0.15 S·cm-1 were found to be best for `Russet Burbank'. Plantlet growth was reduced by low solution pH (3.0) and high EC level (0.36 S·cm-1). Photosynthetic rate, stomatal conductance, and transpiration rate were also found to be affected by EC levels. Down regulation of photosynthesis, as indicated by chlorophyll fluorescence results, were observed when potato plantlets were cultured under nutrient solution of higher EC level. Plantlet growth and photosynthetic rate increased as photosynthetic photon flux (PPF) levels increased from 50 to 250 μmol·m-2·s-1. Particularly, increasing PPF level had a more distinctive effect on plantlet growth than CO2 enrichment condition. It was apparent from this study that nutrient solution of pH 6.0 and 0.15 S·cm-1 EC in combination with high PPF level (250 μmol·m-2·s-1) were suitable for hydroponic culture of potato plantlets as it would maximize net photosynthetic rate, and achieve the highest growth rates.

Open access

Ruining Li, Jiahuan Long, Yongzhe Yan, Jiaming Luo, Zhigang Xu, and Xiaoying Liu

Potato tuber is rich in starch, proteins, and other important nutrients, making potato ( Solanum tuberosum L.) one of the most important staple food and vegetable crops ( Abelenda et al., 2019 ). However, potato crops are susceptible to viral and

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Robert P. Sabba and Bill B. Dean

Potato tubers (Solanum tuberosum) of genotypes that vary in resistance to dark pigment formation when damaged, characteristic of the physiological disorder blackspot, were assayed for free tyrosine. The tubers were also assayed for relative levels of chorismate mutase and proteinase activities, which can regulate free tyrosine levels. The susceptibility of potato tubers to blackspot was shown to be correlated to the amount of free tyrosine by third order regression (R = 0.88). Tyrosine was found to be a limiting factor in pigment development. Chorismate mutase activity (CMI and CMII) was not correlated to blackspot susceptibility of the genotypes studied. Proteinase activities of Atlantic, TXA 763-5, Ranger Russet, Russet Burbank, and Lemhi Russet tuber protein extracts measured with synthetic substrates correlated with blackspot susceptibility. This suggests that the high free tyrosine levels associated with blackspot susceptibility may be due to high levels of proteinase activity in the tuber, rather than tyrosine synthesis.

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Gerson R. de L. Fortes, Rosilene França, and Adriana C. M. Dantas

This work was carried out in the Tissue Culture Laboratory of Embrapa Temperate Climate aiming to maximize the protocol for in vitro culture of potato cv. Baronesa. The treatments consisted of multiplication of microcuttings with one, two, or three buds with/without leaves and originated from different regions of the shoot: apical, middle, or basal. Each treatment was repeated five times with each replication composed of five explants that were inoculated in 250-ml flasks with 40 ml of the medium containing MS salts and vitamins added to: sucrose (30 g·L-1), myo-inositol (100 mg·L-1), agar (6 g·L-1). The pH was adjusted to 5.6 before autoclaving. After inoculation, the flasks remained in a growth room at 25 ± 2 °C, 16-h photoperiod, and 19 μmol·m-2·s-1 light intensity provided by cool-white fluorescents lamps. Observations were done every 5 days. Final evaluation was performed after 30 days. It was observed that basal microcuttings provided longer shoots and that microcuttings with leaves bore the best ones. This kind of explant also favored a higher number of shoots, axilary buds, and better multiplication rate. The presence of leaves in the microcutting is important when basal explants are used once it can improve the number of axillary buds and the rate of multiplication. The higher the number of buds in the microcutting the lower the rate of multiplication. The in vitro multiplication of potato could be improved by using one-leaf bud basal microcutting.

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Tyann Blessington*, Anna L. Hale, Douglas C. Scheuring, and J. Creighton Miller Jr.

We have demonstrated that potatoes contain significant levels of antioxidants important to human health; however, since potatoes are not consumed raw, it is important to determine the effects of cooking/processing on these levels. Therefore, the changes in phenolic and carotenoid content and total antioxidant activity in potatoes were investigated using combinations of storage and cooking methods. Fresh and stored tubers (110 days at 4 °C) of 17 potato cultivars, both raw and cooked (microwaved, boiled, baked, fried), were analyzed for antioxidant activity using the DPPH method. In addition, carotenoid levels were determined for each treatment based on the absorbance of the methanol extraction (oxygenated phenolics and carotenoids) at 445 nm and the hexane extraction (non-oxygenated carotenoids) at 450 nm. Total antioxidant activity as well as carotenoid levels were significantly affected by both genotype and cooking method. Across extraction methods, the microwave and fry cooking treatments were generally highest in antioxidant activity, while boiling was the lowest. Oxygenated carotenoids were significantly affected by storage, while the non-oxygenated carotenoids were unaffected.

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Lavanya Reddivari and J. Creighton Miller Jr.

Antioxidants have been widely reported to play an important role in disease prevention. In addition to preventing cancer, stroke, heart diseases, and inflammation, they are also involved in immune surveillance. Since the per capita consumption of potatoes in the U.S. is about 137 lb, even moderate levels of antioxidants in this most important vegetable crop probably have an important human health benefit. About 75% to 80% of antioxidant activity in specialty potatoes is due to phenolics and carotenoids. The objectives of this investigation were to evaluate antioxidant activity and total phenolic and carotenoid content of specialty potato selections from the Texas Potato Variety Development Program, and to identify candidate compounds for cancer cell culture investigations. Potato tubers were also used to identify and quantify individual phenolics and carotenoids. Some 320 specialty selections were screened for antioxidant activity (AA), total phenolic content (TP) and carotenoid content (CC) using DPPH (2,2-Diphenyl-1-picrylhydrazyl), FCR (Folin-Ciocalteu Reagent) and colorimetric assays, respectively. After the initial screening, the top 10% were used for analysis of individual phenolics and carotenoids using HPLC. Wide variability for antioxidant activity, phenolic content, and carotenoid content was found among specialty potato selections, providing evidence for genetic control of theses traits. The specialty selection CO112F2-2P/P (purple flesh, purple skin) had the highest AA (832 μg trolox equivalents/g fw), TP (1553 μg chlorogenic acid equivalents/g fw) and CC (590 μg lutein equivalents/100 g fw). Chlorogenic acid (55% to 60%), caffeic acid (≈5%), gallic acid (18% to 20%), and catechin (18% to 20%) were found to be the most prevalent phenolic acids, and lutein and zeaxanthin were the most prominent carotenoids contributing to antioxidant activity. Gallic acid was identified as the candidate compound for use in cancer cell culture investigations.

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Gerson R. de L. Fortes, Luciana B. Andrade, Janine T.C. Faria, Marisa de F. Oliveira, and Nilvane T.G. Müller

The potato cultivar Cristal recently released by the CPACT/EMBRAPA Breeding Program has high dry matter and low reduce sugars. These are desirable characteristics as industry processing is concerned. Nevertheless, this is a recalcitrant cultivar. The meristem culture is difficult to establish along with a very low multiplication rate. The aim of this work was to improve the multiplication rate for this cultivar. Two-bud microcuttings derived from apical, mid, and basal regions were inoculated in test tubes with 10 ml MS culture media and vitamins as follows; myo-inositol (100 mg·L–1); sucrose (10 g·L–1). No growth regulator was added. All treatments were placed in a growth room in a 16-hour photoperiod; 25 ± 2°C and 2000 lux. One month later, although it was observed that the final growth was more pronounced for basal microcuttings, no difference could be detected for number of shoots and multiplication rate. It was concluded that it makes no difference whatsoever kind of microcutting is used to start the micropropagation process.