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Guojing Li, Yonghua Liu, Jeffrey D. Ehlers, Zhujun Zhu, Xiaohua Wu, Baogeng Wang, and Zhongfu Lu

region (SCAR) or cleaved amplified polymorphism sequences (CAPS), is critical for both applications. The converted SCARs and CAPS are highly reliable, relatively inexpensive, and can be easily manipulated. Thus, they are valuable in practical breeding

Free access

Xuelin Shen, Yanmei Zhang, Zhao Lei, Yibo Lin, Minxu Cao, Yueyu Hang, and Xiaoqin Sun

amplified fragment length polymorphism molecular markers. Li et al. (2008) screened molecular markers with high efficiency in the identification of accession ‘Youdonger’ in Hangzhou. The other exception was by our research team. We developed a pair of ISSR-SCAR

Full access

Yanmei Zhang, Xuelin Shen, Xiaoqin Sun, Jia Liu, Yifeng Xia, Xin Zou, and Yueyu Hang

were examined by 2.0% agarose gel electrophoresis under 90 V for 1 h and visualized via gel imaging system. Design and validation of a RAPD-SCAR marker. A band specific to ‘Heshangling’ was excised and extracted from the gel. The obtained DNA fragment

Free access

Federico L. Iñiguez-Luy, Michell E. Sass, Geunhwa Jung, Mitrick A. Johns, and James Nienhuis

This research describes the development of robust molecular descriptors that are reliable and easy to use (PCR-based) for the proper classification of the six cultivated Brassica L. species and subspecies that make up the U triangle. Sequence characterized amplified regions (SCAR) were derived from DNA fragments generated by randomly amplified polymorphic DNA (RAPD) primers that distinguished the U-triangle Brassica species: B. rapa L. (A, n = 10), B. nigra (L.) Koch (B, n = 8), B. oleracea L. (C, n = 9), B. juncea (L.) Czern (AB, n = 18), B. napus L. (AC, n = 19), and B. carinata Braun (BC, n = 17). The SCAR descriptors developed in this study reveal the presence-absence of a DNA fragment and sequence length polymorphisms to distinguish the three genomes (A, B, and C) of the six cultivated Brassica species. These markers should prove to be useful in revealing misclassifications at the subspecies level. The amplification of a single DNA fragment and reduced sensitivity to reaction conditions make these SCAR descriptors ideal to accurately classify and organize large numbers of cultivated Brassica accessions typically present in large germplasm collections.

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K.M. Haymes, W.E. Van de Weg, P. Arens, J.L. Maas, B. Vosman, and A.P.M. Den Nijs

Two dominant sequence characterized amplified region (SCAR) markers (linked at 3.0 cM, coupling phase) were constructed for the strawberry (Fragaria ×ananassa Duch.) gene Rpf1. This gene confers resistance to red stele root rot, caused by the soil-born fungus Phytophthora fragariae Hickman var. fragariae. The SCAR markers were developed originally from the sequence of RAPD OPO-16C(438) that is linked in repulsion phase to the Rpf1 allele. This SCAR primer set produced multiple bands in the resistant test progeny and in some of the susceptible progeny; therefore, new SCARs were developed based on the sequence differences among these bands. These new SCARs were linked in coupling phase to the Rpf allele and mapped to the same location as the original RAPD OPO-16C(438). The SCAR markers, as well as some additional RAPD markers known to be linked to Rpf1, were shown to be highly conserved in linkage to the gene based on examination of 133 European and North American Fragaria L. sp. cultivars and breeding selections. These flanking RAPD and SCAR-PCR markers can be used in breeding programs for the selection of red stele (Rpf1) resistance.

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J.H.M. Barten, J.W. Scott, N. Kedar, and Y. Elkind

Abbreviations: BSI, blossom-end scar index; RBS, rough blossom-end scarring. Florida Agricultural Experiment Station Journal Series no. R-01518. This research was supported by grant no. US-993-85 from BARD, The United States-Israel Binational

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S.M. Smith, J.W. Scott, J.A. Bartz, and S.A. Sargent

lycopersicum L. formerly Lycopersicon esculentum Mill) (see Peralta et al., 2006 ) that can be inoculated by water absorption through the stem scar when the fruit are immersed in the water of a packinghouse dump tank. Processed tomatoes do not typically

Open access

Melinda A. Miller-Butler, Barbara J. Smith, Brian R. Kreiser, and Eugene K. Blythe

subgenome-specific locus FaRCa1 conferring resistance to C. acutatum ( Salinas et al., 2018 ). SCAR markers were used to demonstrate a resistant:susceptible segregation ratio of 1:1 in strawberry progeny from crosses between red stele

Free access

Jean-Guy Parent and Daniele Page

Random amplified polymorphic DNA (RAPD) markers are used in Quebec's certification program to verify the identity of raspberry cultivars. However, sequence characterized amplified region (SCAR) markers, less sensitive to modifications in reaction conditions, could be derived from RAPD markers. Our objective was to evaluate the potential of SCAR markers to replace the RAPD ones. Five RAPD markers obtained with primer OPG06 (length of 520, 700, 825, 1450, and 2000 bp) were cloned in pTZ/PC or pCRII vectors. Extremities of the cloned markers were sequenced by the nonradioactive silver sequence method using pUC/M13 forward and reverse primers. Sequence information was used to make SCAR primers, similar in length to standard PCR primers. Some SCAR primers were elongated RAPD primers, whereas others were from internal regions. Ability of primer pairs and combination of primer pairs to discriminate cultivars of our certification program was compared with their RAPD counterparts as well as with the technical feasibility of both methods.

Free access

Margaret R. Pooler, Louise G.H. Riedel, S.E. Bentz, and A.M. Townsend

funded in part by USDA Forest Service Forest Health Technology Enterprise Team Agreement 11-2442-97-05, and by the International Society of Arboriculture Research Trust. We thank Clay Deming and Rosemary Bordley for technical support with RAPD and SCAR