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Emilio Nicolás, Trinitario Ferrandez, José Salvador Rubio, Juan José Alarcón, and Ma Jesús Sánchez-Blanco

use of these species is found in the literature. The use of wild Mediterranean plant species, including Rosmarinus officinalis , has increased and may be an interesting solution because of their good resistance to adverse environmental conditions

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Azza Abdel-Aziz Tawfik and P. E. Read

Regeneration from callus of rosemary has not been reported. Leaf segment, meristem-tip and shoot-tip explants of Rosmarinus officinalis were cultured on a Murashige and Skoog (MS) medium supplemented with five concentrations of the cytokinin thidiazuron (TDZ) alone or in combination with 3-indoleacetic acid (IAA). Callus was formed on the base and leaves of the shoottips after 6 weeks when cultured under cool white fluorescent light (26 u mol·S-1 m-2) on MS containing 0.5, 1.0, 1.5 or 2.0 mg/l TDZ. Calti were transferred to fresh MS medium supplemented with 0.2, 0.4, 0.6, 0.8 or 1.0 mg/l TDZ or 2.0, 4.0, 6.0 or 8.0 mg/l benzyladenine (BA) where shoot formation occurred. Essentiality of IAA was not clear from these experiments and further research is underway to refine regeneration protocol

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Allan M. Armitage, James Garner, and Jimmy S. Greer

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Annika E. Kohler and Roberto G. Lopez

, Israel), and rosemary ‘Arp’ ( Rosmarinus officinalis ) (Dümmen Orange NA, Inc., Columbus, OH) were grown in 15.3-cm (1.3-L; The HC Companies, Twinsburg, OH) round containers filled with a 70% peat, 21% perlite, and 9% vermiculite mixture (Suremix

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Iro Kokkinou, Nikolaos Ntoulas, Panayiotis A. Nektarios, and Dimitra Varela

able to survive for 2 weeks which was expected due to the shallow depth of the green roof substrate layer. Rosmarinus officinalis. The slope of the moisture curve reduction of the nonirrigated plants was similar to that of helichrysum and Greek

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Kwang Jin Kim, Myeong Il Jeong, Dong Woo Lee, Jeong Seob Song, Hyoung Deug Kim, Eun Ha Yoo, Sun Jin Jeong, Seung Won Han, Stanley J. Kays, Young-Wook Lim, and Ho-Hyun Kim

formaldehyde/cm 2 of leaf area over 5 h and was the most effective of the 86 species tested. In contrast, D. deremensis was the least effective. Of the herbs, Lavandula spp., Pelargonium spp., and Rosmarinus officinalis were the most effective in

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Weiguang Yi and Hazel Y. Wetzstein

of drying conditions and extraction protocols on total phenolic content and antioxidant capacity of selected herbs. Materials and Methods Plant materials, harvesting, and drying. Three herbs, rosemary ( Rosmarinus officinalis ), motherwort ( Leonurus

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Shuyang Zhen, Stephanie E. Burnett, Michael E. Day, and Marc W. van Iersel

use water to produce biomass. We selected three important horticultural species for which water requirements have not been quantified. Rosemary ( Rosmarinus officinalis ) is an evergreen shrub/subshrub native to the Mediterranean region that is widely

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Hany M. El-Naggar, Paul E. Read, and Susan L. Cuppett

Rosemary Rosmarinus officinalis is a member of the Lamiaceae. Rosmarinic acid (RA) is a very strong antioxidant produced in the chloroplast, and used to protect plant tissues against oxidative stress. A number of investigations showed that the sucrose concentration in the callus growing medium greatly influenced the production of secondary metabolites of the phenylpropanoid pathway such as RA. The aim of this study was to test the effect of elevated sucrose concentrations (2%, 3%, 4%, 5%, and 6% sucrose) and the effect of light and dark treatments on the production of RA in the callus of five different genotypes. The genotypes were Majorca, Rosmarinus officinalis, Pine Scented, Madeline Hill, and APR. It was found that the dark treatment produces more RA than the light treatment in all genotypes, and in all sucrose concentrations. The RA concentration increased with increasing the sucrose concentration from 2%—reaching the highest concentration at 4% and 5% in most genotypes. The RA concentration declined again at 6% sucrose in all genotypes. We concluded that for the extraction of RA from rosemary callus it is preferred to be produced in the dark—this will save energy and will produce more RA than the light treatment. Also it is preferred to use sucrose concentration at 4% for genotypes Rosmarinus officinalis, Pine Scented, and APR; and 3% sucrose for genotype Madeline Hill in the dark condition. While for the light condition, it is preferred to use 5% sucrose with genotypes Majorca, Rosmarinus officinalis, Pine Scented, and Madeline Hill; and 4% sucrose for genotype APR.

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Jacqueline A. Ricotta and John B. Masiunas

Black polyethylene mulch and weed control strategies were evaluated for potential use by small acreage herb producers. In both 1988 and 1989, the mulch greatly increased fresh and dry weight yields of basil (Ocimum basilicum L.) and rosemary (Rosmarinus officinalis L.). Parsley (Petroselinum crispum Nym.) yield did not respond to the mulch. Preplant application of napropamide provided weed control for 2 weeks, but was subsequently not effective on a heavy infestation of purslane (Portulaca oleracea L.). Hand-hoed and glyphosate-treated plots (both with and without plastic) produced equivalent yields. Chemical names used: N, N -diethyl-2(1-napthalenoxy)-propanamide (napropamide); N- (phosphonomethyl) glycine (glyphosate).