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Yihua Chen, Peng Jiang, Shivegowda Thammannagowda, Haiying Liang and H. Dayton Wilde

family of peach with that of other species of Rosaceae. These studies allowed us to examine the reliability of applying genetic information from peach to other woody rosaceous plants. Materials and Methods Plant materials and nucleic acid preparation

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Joseph J. Rothleutner, Mara W. Friddle and Ryan N. Contreras

greater because this figure accounted only for sales of Cotoneasters classified as “broadleaf evergreens” and many species are deciduous or semievergreen depending on climate and environmental factors. Cotoneaster is a member of Rosaceae, subfamily

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Chengyan Yue, R. Karina Gallardo, James Luby, Alicia Rihn, James R. McFerson, Vicki McCracken, Vance M. Whitaker, Chad E. Finn, James F. Hancock, Cholani Weebadde, Audrey Sebolt and Amy Iezzoni

Trademark Office, 2013 ). This research is a portion of a larger U.S. Department of Agriculture-funded project called “RosBREED: Enabling marker-assisted breeding in Rosaceae.” The overall goal of RosBREED is to facilitate the use of DNA marker

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Joseph J. Rothleutner, Ryan N. Contreras, Virginia O. Stockwell and James S. Owen

apple orchards (B. Duffy, personal communication). Erwinia amylovora is native to the United States and has a host range limited within members of Rosaceae, most commonly affecting members of the apple subfamily (Maloideae) ( van der Zwet and Beer, 1999

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Dwight S. Fujii and Abhaya M. Dandekar

Many tree crops belonging to the Rosaceae family translocate and metabolize sorbitol. We have determined that some species of bacteria belonging to the genus Agrobacterium, Pseudomonas, and Erwinia pathogenic to the Rosaceae demonstrate the ability to metabolize sorbitol while those that were isolated from other hosts could not utilize sorbitol. Employing cellulose acetate electrophoresis (CAE) we have been able to demonstrate the presence of isoenzymes of sorbitol dehydrogenase (SDH) that correlate with the ability to metabolize sorbitol in these organisms. In order to study the properties of SDH in these organisms we carried out a detailed enzymatic analysis of the enzyme from A. tumefaciens. We found that the enzyme displayed activity when mannitol or xylitol were used as substrates, in addition to sorbitol. Michaelis constants (Km) were 32.8 mM, 0.19 mM, and 38.2 mM for sorbitol, mannitol, and xylitol respectively. To further distinguish the reactions with the different substrates the enzymatic extracts were further characterized on CAE using different substrates to visualize patterns of isoenzymes for a particular sugar alcohol. These analyses revealed the presence of unique isoenzymes for SDH. In addition we observed the presence of mannitol dehydrogenase (MDH) representing in most species a non-specific polyol dehydrogenase.

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Mary Woodhead, Ailsa Weir, Kay Smith, Susan McCallum, Katrin MacKenzie and Julie Graham

sequences, and quantitative trait loci (QTL) that can be employed within traditional plant breeding programs. Phylogenetic analyses divide the Rosaceae into three subfamilies, Dryadoideae, Rosoideae, and Spiraeoideae ( Potter et al., 2006 ). Commercially

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Jin Jiao, Xing Liu, Juyou Wu, Guohua Xu and Shaoling Zhang

nonmodel plants such as rice (16 MAPK) ( Liu and Xue, 2007 ), apple (26 MAPK), and mulberry [ Morus notabilis (10 MAPK)] ( Wei et al., 2014 ; Zhang et al., 2013 ). In comparison with that in other species, the MAPK gene family in Rosaceae has not been

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Jonathan D. Mahoney, Thao M. Hau, Bryan A. Connolly and Mark H. Brand

Native to eastern regions in North America, the genus Aronia is a group of deciduous shrubs in the Rosaceae family, subtribe Pyrinae. The Pyrinae subtribe has a base chromosome count of n = 17 ( Postman, 2011 ), and Aronia species are commonly

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Philip J. Stewart, Ashley R. Winslow and Kevin M. Folta

The transition from vegetative growth to reproductive growth is carefully controlled by a number of independent signal transduction systems, one of which interprets photoperiod. Photoperiodic control of flowering time has been well-described in Arabidopsis and rice, revealing the presence of a generally common network of regulatory proteins. Timely and appropriate progression to flowering is critical to profitable production of cultivated strawberry (Fragaria ×ananassa), a species that includes long-day, short-day, and day-neutral cultivars. In an effort to characterize the photoperiodic flowering control mechanism in strawberry, the Fragaria orthologs of the photoperiod pathway genes were cloned and sequenced. Strawberry versions of Constans, Constans-like, Leafy, Flowering Locus T, and Suppressor of Constans Overexpression 1 were identified by screening cDNA libraries and through degenerate PCR approaches. Expression of these transcripts in short-day and day-neutral cultivars was tested under long and short photoperiods. Functional complementation of Arabidopsis mutants was performed where appropriate, alleles were identified, genetic linkage was determined where possible, and relationships between the strawberry genes and homologs from other species were studied. These trials define the mechanistic elements of an agriculturally important pathway in this valuable crop, and lays the foundation for transgenic studies in strawberry to manipulate the floral transition.

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Le Luo, Chao Yu, Xuelian Guo, Huitang Pan and Qixiang Zhang

grain was wide in the center and narrow at the ends, but this was not the case for prolate or perprolate pollen grains. Previous research on plant geography in Xinjiang ( Hui et al., 2003 ) found primitive, transitive, and evolved Rosaceae communities