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R.J. Griesbach, R.M. Beck, and J.R. Stehmann

A method was developed to characterize the genetic heterogeneity of the chalcone synthase gene intron within the Petunia integrifolia (Hook.) Schinz & Thell. species complex. The DNA from wild species collected from known locations was used to amplify the chalcone synthase gene intron through the polymerase chain reaction (PCR). The resulting PCR product was then characterized by Rsa 1 restriction, revealing a degree of heterogeneity that could be used to characterize the species genetically. Of the four different species that were characterized, two could be placed in the same genetic grouping. This study shows that the variation in the intron of the Chs A gene may be species-specific.

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Ryan M. Warner

and P. integrifolia ( Stehmann et al., 2009 ), and Petunia spp. are generally cross-compatible ( Ando et al., 2001 ; Watanabe et al., 1996 , 2001 ), although fertility varies widely between parental species combinations. Little information is

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Xinwang Zhang, Ikuo Nakamura, and Masahiro Mii

, Sterns & Poggenb. subsp. axillaris , after current treatment] with white flowers and P. violacea Lindl. [= P. integrifolia (HOOK.) Schiz & Thell.] with purple flowers ( Ferguson and Ottley, 1932 ). Petunia inflata R. E. Fr with purple flowers and P

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Aaron E. Walworth and Ryan M. Warner

. hybrida ; and 2) to evaluate cold acclimation of P. axillaris , P. integrifolia , P. exserta , and P. hybrida ‘Mitchell’ in an effort to identify genetic material that may be useful in breeding more stress-tolerant cultivars. Materials and Methods

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R.J. Griesbach and R.M. Beck

The sequence of the intron within the chalcone synthase A gene (ChsA) was used to characterize Petunia integrifolia subsp. integrifolia var. depauperata (Fries) Smith et Downs, P. altiplana Ando et Hashimoto, P. littoralis Smith et Downs, and an unknown taxon from the town of Torres in Brazil. Based upon the intron, the Torres taxon most closely resembled P. integrifolia. The unrooted phylogenetic tree suggested that P. integrifolia was more closely related to P. littoralis than P. altiplana.

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Robert Griesbach* and Ronald Beck

The genetic distance for three Petunia species was determined based upon an intron in the chalcone synthase gene. The sequence of the intron was obtained for P. integriflolia ssp. integriflolia var. depauperata, P. integriflolia ssp. integriflolia Torres ecotype, P. altiplana and P. littoralis. These species are very closely related and believed by some taxonomists to be part of a large single species complex. In all the taxa, the intron contained multiple repeated and inverted sequences. The P. integriflolia ssp. integriflolia Torres ecotype intron differed from the P. integriflolia subsp. integriflolia var. depauperata intron in 3 of 930 nucleotides. While, the P. littoralis intron differed from the P. integrifolia subsp. integrifolia var. depauperata intron in 15 of 930 nucleotides. As compared to the P. integrifolia subsp. integriflolia var. depauperata intron, the intron in P. altiplana intron was longer (1125 bp), had a section of 338 nucleotides with a completely different sequence, and differed by 27 of 787 nucleotides in the common sequence.

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Teresa A. Cerny and Terri W. Starman

Seed of five species of petunia and 10 cultivars of Petunia xhybrida were obtained from several sources and plants were fingerprinted using DNA amplification fingerprinting (DAF). Within some species, variable fingerprints were generated between individual plants from the same seed source and/or different sources. Consistencies were found among DAF profiles by bulking the leaf tissue from 10 different plants, but not five plants. Each of 10 octamer primers used during the study revealed polymorphic loci between the species and cultivars. Among the 201 bands produced, 146 (73%) loci were polymorphic and these could be used to distinguish between each of the species and cultivars. Scoring for presence and absence of the amplified bands was used to generate a phylogenetic tree and to calculate the pairwise distances between each of the taxa using parsimony (PAUP) analysis. The tree generated using DAF molecular markers separated P. axillaris from P. parodii (two white-flowered species), and distinguished between the violet-flowered species, P, inflata, P. integrifolia, and P. violacea.

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Ryan M. Warner

temperature response coefficients for the rate of progress toward flowering for the species ranged from 0.0010 for P. exserta and petunia to 0.0015 for P. integrifolia , which was narrower than the range of 0.00096 to 0.0021 exhibited by the cultivars in

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Kimberly A. Cochran and Craig S. Rothrock

13.8 °C and 24.0 to 10 °C for 2007 and 2008, respectively. Crop production. Petunia ( Petunia axillaries × P. integrifolia Schinz & Thell) ‘Bobby Dazzler’ and impatiens ( Impatiens wallerana L.) ‘Dazzler Mix’ were selected for use in this study due