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Kellie J. Walters and Christopher J. Currey

, schedule crops, and conduct cost–benefit analyses. Materials and Methods Plant material and propagation. Seeds of sweet basil ( O. basilicum ‘Nufar’), holy basil ( O. tenuiflorum ), and lemon basil ( O. × citriodorum ‘Lime’ and O. basilicum ‘Sweet Dani

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Kellie J. Walters and Christopher J. Currey

Basil (Ocimum sp.) is the most popular fresh culinary herb. However, there is a lack of data characterizing the effect of hydroponic production systems and cultivars on the yield of hydroponically produced basil. Our objectives were to quantify productivity and characterize growth of basil cultivars grown in two hydroponic production systems. Thirty-five basil cultivars, including selections of sweet basil (O. basilicum), holy basil (O. tenuiflorum), and lemon basil (O. ×citriodorum and O. basilicum) were chosen. Seedlings were transplanted into nutrient film technique (NFT) or deep flow technique (DFT) systems and grown for 3 weeks. There was no interaction between basil cultivars and hydroponic production system. Fresh weight of plants grown in DFT systems was 2.6 g greater compared with plants grown in NFT systems. Basil cultivars differed greatly in fresh weight. In general, holy, lemon, and sweet basil cultivars produce moderate to high fresh weight, but vary greatly. Dissimilarly, bush (O. basilicum var. minimum), cinnamon (O. basilicum), large-leaf (O. basilicum), and thai basils (O. basilicum var. thyrisiflorum) produce moderate fresh weight and purple basil (O. basilicum) cultivars produce the least fresh weight. The yield of basil seems to be affected more by cultivar selection than hydroponic production system. Therefore, hydroponic basil producers should select basil cultivars based on flavor and yield, while hydroponic systems should be selected based on operational preferences.

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Kellie J. Walters and Christopher J. Currey

0.2% and 0.3%, respectively ( Fig. 1 ), as EC increased from 0.5 to 4.0 dS·m −1 . Fig. 1. ( A−L ) Tissue nitrogen (N), phosphorus (P), magnesium (Mg), and calcium (Ca) concentrations of sweet basil ( Ocimum basilicum ‘Nufar’), lemon basil ( O

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Roberto F. Vieira, Peter Goldsbrough, and James E. Simon

Molecular markers were used to assess genetic diversity in basil (Ocimum L. spp., Lamiaceae). Using randomly amplified polymorphic DNA (RAPD) analysis, 11 primers generated 98 polymorphic bands, ranging from 300 to 2,000 base pairs, that discriminated among 37 accessions across nine Ocimum spp. Means of genetic similarities within Ocimum spp. showed that the domesticated species, O. minimum L. (0.887), O. basilicum L. (0.769), and O. ×citriodorum Vis. (0.711) had highest similarity indices within species, while the nondomesticated, O. americanum L. (0.580), O. gratissimum L. (0.408), and O. kilimandscharicum Guerke (0.559) showed the lowest similarity. RAPD results indicated that O. minimum should not be considered a distinct species but rather a variety of O. basilicum. Consistent clusters among all but one of the O. ×citriodorum spp., all containing citral as the major constituent, were identified using bootstrap analysis. RAPD analysis was useful in discriminating among Ocimum spp., although within species resolution will require a higher number of polymorphic bands.

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Christian A. Wyenandt, James E. Simon, Margaret T. McGrath, and Daniel L. Ward

some instances, chlorosis resulting from downy mildew was visible on O. citriodorum , but no sporulation was present on the underside of leaves in cultivars such as Lemon Std. or only very light sporulation was visible on the margins of older

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Robert M. Pyne, Adolfina R. Koroch, Christian A. Wyenandt, and James E. Simon

mildew was first reported in 2010 from field evaluations in which all O. basilicum cultivars and breeding lines were highly susceptible ( Wyenandt et al., 2010 ). Sporulation was reduced in multiple cultivars of O. × citriodorum , while sporulation