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Po-Hung Wu and Doris C.N. Chang

., 2000 ). Using plant growth substances such as GA 3 or N-6-benzyladenine (BA) separately at high temperatures failed to induce phalaenopsis to spike or bloom ( Chen et al., 1994 , 1997 ; Kubota et al., 1997 ). However, Chen et al. (1994) injection

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Martín Mata-Rosas and Víctor M. Salazar-Rojas

shoot formation. ( A ) Flowering plant. ( B ) Multiple shoot formation from protocorms in Murashige and Skoog medium + 13.3 μM N 6 -benzyladenine. ( C ) In vitro-rooted shoots ready for ex vitro culture. ( D ) Plants obtained from in vitro culture after

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Milorad Vujičić, Aneta Sabovljević, Jasmina Šinžar-Sekulić, Marijana Skorić, and Marko Sabovljević

among treatment according to least significant difference ( lsd ) test ( lsd 0.05 =11.74, P < 0.05) IBA = indole-3-butyric acid; BA = N 6 -benzyladenine; MS = Murashige and Skoog. Auxins and cytokinins have basic functions in the regulation of normal

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Yung-I Lee

explant of Cypripedium formosanum at quarter-strength Murashige and Skoog medium supplemented with 4.44 μM N 6 -benzyladenine. Explant survival percentage = number of explant survival/number of explant cultured × 100%; shoot-forming percentage = number

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O. Pérez-Tornero, F. Ortín-Párraga, J. Egea, and L. Burgos

Apricot (Prunus armeniaca L. cv.'Helena') shoots grown on a proliferation medium containing 3% sucrose, 0.4 mg·L–1 benzyladenine (BA), and 0.04 mg·L–1 indolebutyric acid (IBA) and solidified with 0.6% agar were stored at three different temperatures in the dark for up to 24 weeks. All shoots remained viable for 24 weeks when stored at 3 °C, while at 14 °C the percentage of survival decreased quickly after 12 weeks of storage. At 7 °C, percentage of survival started to decline after 18 weeks of storage. Shoots stored at 3 °C had the highest regeneration rates and shoot lengths following transfer to standard proliferation conditions. This temperature also had a beneficial effect on shoot proliferation during the first 12 to 18 weeks of the experiment.

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Kazuhiko Mitsukuri, Takaya Arita, Masahumi Johkan, Satoshi Yamasaki, Kei-ichiro Mishiba, and Masayuki Oda

) containing 30 g·L −1 sucrose and 8 g·L −1 agar (Wako Pure Chemical Industries, Ltd., Osaka, Japan) (pH 5.6) supplemented with 4.44 μM N 6 -benzyladenine and 0.54 μM α-naphthaleneacetic acid. This combination and concentration of plant growth regulators were

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Marko Sabovljević, Milorad Vujičić, Jasmina Šinžar Sekulić, Jose Gabriel Segarra-Moragues, Beata Papp, Marijana Skorić, Luka Dragačević, and Aneta Sabovljević

least significant difference ( lsd ) test ( lsd 0.05 = 11.74, P ≤ 0.05). MS = Murashige and Skoog; IBA = indole-3-butyric acid; BA = N 6 -benzyladenine. The growth of secondary protonema was more positively affected by the simultaneous addition of IBA

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Chia-Yun Ko, Tsai-Yun Lin, Chin-Wen Ho, and Jei-Fu Shaw

condition on C. follicularis shoot proliferation, different amounts of indole 3-acetic acid (IAA) and N6-benzyladenine (BA) were applied. Each sample of subcultured explant (2.0 ± 0.1 g) was cultured in one-fifth MLMS or one-tenth MLMS medium with four

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Martín Mata-Rosas, Rosario Julieta Baltazar-García, and Victor Manuel Chávez-Avila

; BA = N 6 -benzyladenine. Despite the importance of Oncidium in horticulture, there are few studies concerning in vitro propagation, but most studies focus on artificial hybrids, not species. Commonly used explants for micropropagation are

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M.L. Arrebola, O. Socorro, A. Barceló-Muñoz, E. Simón-Pérez, and Fernando Pliego-Alfaro

A micropropagation procedure for juvenile and adult savory (Satureja obovata Lag.) explants is described. Pretreatment of the nutlets with gibberellic acid (0.57 mm) did not improve in vitro germination. Optimum shoot proliferation of juvenile and adult material was obtained on medium containing 2.22 μm N6-benzyladenine. Rooting and acclimatization of juvenile shoots were accomplished in vivo, while adult shoots were rooted in vitro after 3 days of exposure to 4.92 μm indole-3-butyric acid followed by subsequent transfer to auxin-free medium. More than 95% survival of adult rooted plants was obtained during the acclimatization phase. Chemical names used: gibberellic acid (GA3); N6-benzyladenine (BA); indole-3-butyric acid (IBA); isopentenyladenine (2iP).