Maloideae, and appears to be most closely related to Pyracantha (firethorn) and Heteromeles (christmas berry) ( Robertson et al., 1991 ; Rohrer et al., 1992 ). Taxonomy at the family level is complicated, with interspecific and intergeneric
Joseph J. Rothleutner, Mara W. Friddle and Ryan N. Contreras
Joseph J. Rothleutner, Ryan N. Contreras, Virginia O. Stockwell and James S. Owen
apple orchards (B. Duffy, personal communication). Erwinia amylovora is native to the United States and has a host range limited within members of Rosaceae, most commonly affecting members of the apple subfamily (Maloideae) ( van der Zwet and Beer, 1999
Y. Wakasa, R. Ishikawa, M. Niizeki, T. Harada, S. Jin, M. Senda and S. Akada
Sequencing of the sequence tagged site-DNA marker fragments linked to the fruit skin-color gene in apple revealed two fragments containing insertions that are associated with yellow skin color, and are alleles of the red skin gene (Rf). One fragment resulted from the insertion of a 76-bp inverted repeat, whereas the 163 bp in the other fragment was characterized as a mobile element because of the presence of target site duplication. A database search found this latter element in the 5' flanking regions or intron of six apple genes. DNA blot analysis revealed that the element is highly reiterated, with a copy number of between 5000-6000 in the genome of Maloideae (pome) fruit trees. We named this element Majin. Inter-transposon amplified polymorphism using primers from this element resulted in polymorphic bands among apple cultivars.
Xiaoying Li, Hongxia Xu, Jianjun Feng and Junwei Chen
loquat, analyze their distribution regularity, and design primers to test the authenticity and identify the transferability of these SSR loci in other Maloideae species of the Rosaceae. These results will prove very useful for the evaluation of genetic
Andrew C. Bell, Thomas G. Ranney, Thomas A. Eaker and Turner B. Sutton
Fire blight, caused by the bacterium Erwinia amylovora (Burrill) Winslow et al., is one of the most destructive diseases of plants in the Rosaceae subfamily Maloideae. Artificial inoculations, using E. amylovora strain E2002a, were conducted to determine levels of resistance to fire blight among taxa of flowering pears (Pyrus L. spp.) and quince (Chaenomeles Lindl. spp.). The level of resistance was measured as the length of the fire blight lesion as a percentage of overall shoot length. Considerable variation in resistance was observed among both pears and quince. Pyrus ussuriensis Maxim. `Prairie Gem' was highly resistant with a lesion length of 1% of the total shoot length. Pyrus calleryana Decne. `Bradford' was intermediate with a 50% lesion length while P. calleryana `Chanticleer' was significantly more resistant with a lesion length of 31%. Nine pear taxa were highly susceptible and did not differ significantly from 100% disease severity (total shoot death). Chaenomeles speciosa (Sweet) Nak. `Contorta' was highly resistant with a lesion length of 15%. Six quince taxa, including C. × superba (Frahm) Rehd. `Cameo', `Texas Scarlet', and `Jet Trail' were highly susceptible while nine other taxa showed intermediate resistance.
Tomoya Esumi, Ryutaro Tao and Keizo Yonemori
Japanese pear (Pyrus pyrifolia) and quince (Cydonia oblonga), both classified in the subfamily Maloideae, show differences in inflorescence architectures despite of the fact that they are genetically closely related. We previously isolated flowering related genes, LEAFY (LFY) and TERMINAL FLOWER 1 (TFL1) homologues, from these species and showed that they had two types of homologues for each gene. In this study, we examined the expression pattern of LFY and TFL1 homologues in these species by in situ hybridization and RT-PCR. The floral bud was dissected to small pieces under stereomicroscope; apical meristem, scales/bracts, pith, floral meristem, and inflorescence; and then used for RT-PCR. The LFY homologues were expressed in apical meristem and scales/bracts before the floral differentiation in both Japanese pear and quince. After floral differentiation, the expression was observed in floral meristem, scales/bracts and pith in both the species. The TFL1 homologues were strongly expressed in the apical meristem, but their expression was drastically decreased just before floral differentiation. It is considered that the decrease of expression of TFL1 homologues is a sign of floral initiation. The expression of TFL1 homologues was transiently increased at the beginning of floral differentiation in both species. Moreover, one of TFL1 homologues in Japanese pear was continuously expressed in the inflorescence part in the floral primordia, whereas expression of TFL1 homologues in quince almost completely disappeared after a solitary floral meristem was initiated. It was suggested that TFL1 homologues may also be involved in the inflorescence development of Japanese pear.
Reut Niska, Martin Goldway and Doron Schneider
Loquat ( Eriobotrya japonica Lindl.) is a subtropical evergreen fruit tree of the family Rosaceae, subfamily Maloideae ( Lin et al., 1999 ), which carries the RNase-dependent gametophytic self-incompatibility fertilization system. This system is
Samuel G. Obae, Mark H. Brand, Bryan A. Connolly, Rochelle R. Beasley and Stacey L. Lance
SSR markers to other taxa within Maloideae subfamily ranged from 58% to 94%. Higher transferability ratios were observed between species within genera (94%) compared with between genera (58% to 81%). Similarly, Fan et al. (2013) reported that 58
Peter J. Leonard, Mark H. Brand, Bryan A. Connolly and Samuel G. Obae
genetic improvement of commercial cultivars of Aronia . The genus Aronia belongs to the subtribe Pyrinae (formerly subfamily Maloideae) that is rife with taxonomic difficulties ( Campbell et al., 2007 ; Potter et al., 2007 ). This group includes
Gayle M. Volk, Adam D. Henk, Christopher M. Richards, Philip L. Forsline and C. Thomas Chao
and implications for linkage mapping with SNPs Tree Genet. Genomes 7 857 868 Phipps, J.B. Robertson, K.R. Rohrer, J.R. Smith, P.G. 1991 Origins and evolution of subfam. Maloideae (Rosaceae) Syst. Bot. 16 303 332 Phipps, J.B. Robertson, K.R. Smith, P