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Nicholas A. George, Kenneth V. Pecota and G. Craig Yencho

selected sweetpotato ( Ipomoea batatas (L) Lam.) varieties and breeding clones, p. 281–286. Scientist and farmer: Partners in research for the 21st century. Program Report, 1999–2000. Program Report 1999–2000. International Potato Center, Lima, Peru Martin

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Joshua K. Craver, Chad T. Miller, Kimberly A. Williams and Nora M. Bello

variety ( Yencho et al., 2012 ). Intumescences on sweetpotato have not been extensively studied, and very few published research articles discuss the development of this disorder on the crop. One paper, “Intumeszenze fogliari di ‘Ipomoea batatas,’” was

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Ming Liu, Aijun Zhang, Xiaoguang Chen, Rong Jin, Hongmin Li and Zhonghou Tang

). Sweetpotato [ Ipomoea batatas (L.) Lam.] is an important human food, animal feed, and industrial raw material, which is grown in more than 100 countries and mainly produced in China ( FAO, 2011 ; Jin, 2012 ). Sweetpotato is a typical “K-favoring” crop ( Tang

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Meng Wei, Aijun Zhang, Hongmin Li, Zhonghou Tang and Xiaoguang Chen

and S deficiency during wheat grain filling J. Expt. Bot. 59 3675 3689 Islam, M.S. Yoshimoto, M. Yahara, S. Okuno, S. Ishiguro, K. Yamakawa, O. 2002 Identification and characterization of foliar polyphenolic composition in sweetpotato ( Ipomoea batatas

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R.L. Jarret, N. Bowen, S. Kresovich and Z. Liu

Simple sequence repeats (SSRs) were isolated from a size-fractionated genomic DNA library of sweetpotato [Ipomoea batatas (L.) Lam.]. Screening of the library with five oligonucleotide probes, including; (GT)11, (AT)11, (CT)11, (GC)11, and (TAA)8, detected the occurrence of 142 positive colonies among ≈12,000 recombinants. Automated DNA sequencing revealed the presence of simple, compound, perfect, and imperfect SSRs. Five homologous PCR primer pairs were synthesized commercially and used to screen 30 sweetpotato clones for the occurrence of SSR polymorphisms. All primer pairs produced an amplification product of the expected size and detected polymorphisms among the genotypes examined. The potential for the use of SSRs as genetic markers for sweetpotato germplasm characterization is discussed.

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Silver Tumwegamire, Regina Kapinga, Patrick R. Rubaihayo, Don R. LaBonte, Wolfgang J. Grüneberg, Gabriela Burgos, Thomas zum Felde, Rosemary Carpio, Elke Pawelzik and Robert O.M. Mwanga

Sweetpotato [ Ipomoea batatas (L.)] ranks fifth in importance for its caloric contribution in developing countries after rice, wheat, maize, and cassava ( CIP, 2005 ). In some areas of EA, the crop has become a staple ( Scott et al., 2000 ). For

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Arthur Villordon, Don La Bonte and Robert Jarret

The presence of copia-like retrotransposon sequences in sweetpotato [Ipomoea batatas (L.) Lam.] was investigated. PCR-based amplification using primers to highly conserved copia-like reverse transcriptase sequences produced several products corresponding to the expected target size (≈300 bp) that were subsequently isolated and cloned. A random sample of the clones were sequenced and all six reading frames were translated into their corresponding amino acid sequences. Sequence analysis revealed the presence of 22 copia-like reverse transcriptase sequences corresponding to various subfamilies. The presence of several sequence families in the genome is indicative of past or recent transposition activity. Southern blot analysis suggested that these copia-like sequences were present in several hundred copies in the sweetpotato genome. Data also showed retrotransposon insertion polymorphisms between a limited sample of virus-tested and virus-infected sweetpotato clones, indicating putative activity and mobility. This investigation documented the presence of copia-like retrotransposon sequences in the sweetpotato genome. This is an important step in clarifying the possible association between mobile genetic elements and the unusually high incidence of somatic mutations that may result in clonal decline in sweetpotato and other asexually propagated crops. Data presented provides information on the possible use of retrotransposons as genetic markers for sweetpotato crop improvement.

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MD. Shahidul Islam*, M. Jalaluddin, M. Yoshimoto and O. Yamakawa

The antibacterial activity of artificially grown sweetpotato [Ipomoea batatas (L.) Lam.] leaves was investigated against both gram positive and gram negative bacteria namely Escherichia coli (O157:H7), Bacillus and Ecolai using three different cultivars, which are developed to use as a leafy vegetables namely Simon-1, Kyushu-119 and Elegant Summer. The sweetpotato leaves were grown under different temperatures (20 °C, 25 °C, and 30 °C) and artificial shading (O%, 40% and 80%) conditions. There were some cultivar differences but the lyophilized leaf powder (100 mg) from all the cultivars in the Trypto Soya Broth cultivation medium (10 mL) strongly suppressed the growth of all the bacteria studied and its effect was detectable even after autoclave treatment. But the antibacterial extract of the leaves had no effect on the growth of five types of bifidobacterium useful for human health. The water extracted antibacterial fractions from all the cultivars were viscous and the color was brown. Furthermore, the leaves grown under moderate low temperature (20 °C) with 0% shading treatments strongly suppressed the bacterial growth as comported to other treatments, which was accompanied by significantly high accumulation of sugar and polyphenol contents in the leaves. The results also suggest that there were a strong relationship among bacterial growth and antioxidatative compounds in the sweetpotato leaves. Therefore, the antibacterial action of sweetpotato leaves may depend on their antioxidative compounds or/and pectin like materials. Thus, the practical use of sweetpotato leaves is expected to prevent bacteria caused food poisoning.

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Allan M. Armitage and James M. Garner

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Rozalyn Pama*, Jay Doronila and Mari Marutani

Fifteen sweetpotato [Ipomoea batatas (L.) Lam] accessions grown on Guam were studied for morphological and genetic characteristics. Accessions, obtained from AVRDC (Asian Vegetable Research and Development Center) in Taiwan, Saipan, Rota, and Guam, were investigated for marketable yield, growth habit and characteristics of tuberous roots (color, shape, sugar content and moisture content). Results of this study were used to determine the morphological relationship of the accessions of sweetpotato. Phenetic analysis revealed four major clusters according to tuberous root characteristics. The genetic relationship of these sweetpotato accessions was also evaluated for genetic differences among accessions. DNA was extracted and went through polymerase chain reaction (PCR). PCR products were analyzed by random amplified polymorphic DNA (RAPD) fingerprinting. Result of the genetic relationship among the sweetpotatoes was compared with the morphology of accessions using UPGMA cluster analysis and principal compounds analysis.