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Yu Bai, Ying Zhou, Xiaoqing Tang, Yu Wang, Fangquan Wang and Jie Yang

development and flowering of I. indigotica . Conclusion Illumina sequencing and de novo assembly were performed for I. indigotica at the reproductive stages. As a result, more than 44.97 Gbp of data were obtained and assembled into 124,508 unigenes with an

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He Huang, Yuting Liu, Ya Pu, Mi Zhang and Silan Dai

absorbance was measured at 663, 645, and 480 nm. Values were computed as described by Aydi et al. (2010) . The malondialdehyde (MDA) content was estimated using a strategy by Heath and Packer (1968) . All experiments were repeated in triplicate. Illumina

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Jianfeng Liu, Bowen Yang, Yuetong Ming, Yuchu Zhang and Yunqing Cheng

time points for each stage was pooled for library preparation and sequencing. The cDNA library preparation and Illumina sequencing for transcriptome analysis were performed as previously described ( Cheng et al., 2015 ). After low-quality and adaptor

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Samuel G. Obae, Mark H. Brand, Bryan A. Connolly, Rochelle R. Beasley and Stacey L. Lance

using a Covaris S220 ultrasonicator (Covaris, Inc., Woburn, MA) and following the standard protocol of the Illumina MiSeq Reagents kit v2 (Illumina, San Diego, CA). Illumina sequencing was conducted on the MiSeq system (Illumina) with 150 bp paired

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Barbara S. Gilmore, Nahla V. Bassil, Danny L. Barney, Brian J. Knaus and Kim E. Hummer

accession RB14 ‘Timperley Early’ was one of eight Illumina ® sequencing libraries that were pooled and sequenced in one lane with the Illumina ® Genome Analyzer II at the Oregon State University Center for Genome Research and Biocomputing (CGRB). Each

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Josh A. Honig, Megan F. Muehlbauer, John M. Capik, Christine Kubik, Jennifer N. Vaiciunas, Shawn A. Mehlenbacher and Thomas J. Molnar

, Applied Biosystems), and sized using LIZ 600 size standard v2.0 (Applied Biosystems) and Genemapper 5.0 software (Applied Biosystems). ddRADseq library construction and Illumina sequencing. ddRADseq libraries for H3R07P25, OSU 1155.009, and 119 mapping

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Colton Ives, Vidyasagar R. Sathuvalli, Brooke C. Colburn and Shawn A. Mehlenbacher

bacterial artificial chromosomes (BACs) using multiplex Illumina sequencing and targeted marker development for eastern filbert blight resistance Tree Genet. Genomes 9 1109 1118 Thompson, M.M. 1979a Genetics of incompatibility in Corylus avellana L Theor

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Michael J. Havey and Yul-Kyun Ahn

, seed production, and the genesis of garlic breeding Plant Breeding Rev. 23 211 244 Sun, X. Zhou, S. Meng, F. Liu, S. 2012 De novo assembly and characterization of the garlic ( Allium sativum ) bud transcriptome by Illumina sequencing Plant Cell Rpt. 31

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Zehuang Zhang, Qihua Lin and Qiuzhen Zhong

that qualified samples were used to carry out transcriptome sequencing ( Shan et al., 2008 ). Construction of cDNA library and illumina sequencing. High-quality RNA samples from chinese bayberry were sent to Biomarker Technologies Corporation (Beijing

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Michael Dossett, Jill M. Bushakra, Barbara Gilmore, Carol A. Koch, Chaim Kempler, Chad E. Finn and Nahla V. Bassil

preparation consisted of: DNA sonication [Bioruptor XL (BR_XL); Diagenode, Denville, NJ]; DNA strand end repair; addition of an adenine base to the 3′ ends; ligation of a custom Illumina ® sequencing adapter (Illumina, San Diego, CA) containing a 4-bp barcode