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Jericó J. Bello-Bello, Lourdes G. Iglesias-Andreu, Susana A. Avilés-Viñas, Eunice Gómez-Uc, Adriana Canto-Flick, and Nancy Santana-Buzzy

molecular techniques are currently available to detect sequence variation between closely related genomes such as those between source plants and somaclones. These include random amplified polymorphic DNA (RAPD), ISSR, microsatellites or simple sequence

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Nicole Gardner and Stan C. Hokanson

The genus Clematis contains many well-known large flowered cultivars, as well as lesser-known nonvining species. Intersimple sequence repeat (ISSR) primers were used to fingerprint 32 vining cultivars and five nonvining species (C. fruiticosa, C. integrifolia, C. heracleifolia, C. hexapetala, and C. recta) for use in assessing genetic relationships and cultivar identification. Four ISSR primers yielded a total of 44 bands in the vining accessions, of which 36 (86%) were polymorphic. The average polymorphism levels were 83% for the cultivars and 94% for the nonvining species. All 32 vining cultivars were distinguished with the use of two ISSR primers, and the five nonvining Clematis species were differentiated with three ISSR primers. A similarity matrix of the cultivars showed low similarity levels between the samples, with an average similarity of 0.28. A UPGMA-derived dendrogram showed no strong groupings among any of the samples. Two cultivars with known parentage, Clematis viticella L. `Betty Corning' and `Sylvia Denny', grouped with one reported parent but not the other, suggesting they are more similar to one parent. `Multi-blue', a sport reportedly arising from `The President' did not segregate near `The President'.

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Kai-Ge Zhao, Ming-Qin Zhou, Long-Qing Chen, Donglin Zhang, and Gituru Wahiti Robert

markers (ISSR) and random amplified polymorphic DNA markers (RAPD) are two commonly used methods, mainly because they are simple to execute, are low cost, and require neither sequence information nor prior genetic studies ( Williams et al., 1990

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Sima Taheri, Thohirah Lee Abdullah, Nur Ashikin Psyquay Abdullah, and Zaiton Ahmad

such as the random amplified polymorphic DNA (RAPD) ( Panda et al., 2007 ; Syamkumar and Sasikumar, 2007 ), ISSR ( Singh et al., 2012 ; Taheri et al., 2012 ), simple sequence repeat (SSR) ( Sigrist et al., 2011 ; Siju et al., 2010 ), and amplified

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Robert R. Krueger and Mikeal L. Roose

The authors acknowledge and thank Osman Gulsen and Lisa Mu for their assistance with the ISSR analysis, and Noelle Barkley, Sabeena Singh, Kimberly Hurd, and Christina Caro for DNA extraction. Mention of a trademark, proprietary product, or vendor

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Liwang Liu, Guang Liu, Yiqin Gong, Wenhao Dai, Yan Wang, Fanmin Yu, and Yunying Ren

from both parents, identification of male and female parent-specific markers will allow differentiation of true hybrids from selfed parental lines and outcrossed lines. Molecular markers, such as RAPD, ISSR, SSR, SRAP, AFLP (amplified fragment length

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Mohammad Sadat-Hosseini, Kourosh Vahdati, and Charles A. Leslie

markers commonly used for the evaluation of somaclonal variation include randomly amplified polymorphic DNA (RAPD), restricted fragment length polymorphism (RFLP), simple sequence repeats (SSRs), and ISSR. Of these, ISSR is the fastest, simplest, and most

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Dong Liu, Ping Li, Jiulong Hu, Kunyuan Li, Zhenyu Zhao, Weiyan Wang, Jinyuan Zhang, Xu Ding, and Zhimou Gao

P. sojae isolates from China and the United States with RAPD and ISSR to clarify the origin of P. sojae . The results did not support the conjecture that P. sojae in the United States. may be derived from China. Recently, RAPD, SSR, and ISSR were

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Ping Li, Dong Liu, Min Guo, Yuemin Pan, Fangxin Chen, Huajian Zhang, and Zhimou Gao

applied ISSR fingerprinting to identify a genetic marker capable of distinguishing between A1 and A2 P. capsici mating types. Materials and Methods Strains and mycelium preparation. Twenty-one P. capsici isolates were collected from infected peppers

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Salih Kafkas, Yıldız Doğan, Ali Sabır, Ali Turan, and Hasbi Seker

Vendramin (2002) used polymerase chain reaction (PCR)-RFLP to look at chloroplast variation in wild hazelnut populations. The objective of this study was to evaluate RAPD, ISSR, and AFLP markers for identifying 18 hazelnut cultivars that are economically