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Jacqueline Joshua and Margaret T. Mmbaga

apples, respectively. Based on previous results, these baits were most effective in the isolation of diverse soilborne pathogens from rhizosphere soil. Morphological characteristics of various Fusarium species isolated from the rhizosphere soil were

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Anas Eranthodi, Mohammad Babadoost and Bernhard Trierweiler

colonies when the sets were treated at 47 °C or greater and cultured within 2 weeks and 3 months after hot water treatment. Verticillium and Fusarium species were identified in the colonies that grew from untreated sets (both asymptomatic and

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Frank A. Buffone and Don R. La Bonte

Chlorotic Leaf Distortion (CLD) is a common disease of sweetpotato caused by Fusarium lateritium. This fungus is unique among Fusarium species in that it grows on the epidermis of leaves and shoot tips of sweetpotato. Fusarium lateritium appears as a white epiphytic material and under bright sunlight causes leaf chlorosis. When cloudy weather persists for several days, all symptoms disappear.

Researchers who use RAPD to examine banding patterns of sweetpotato DNA assume that foreign DNA present in the cTAB extract is quantitatively low and will not appreciably amplify and appear as bands. In this study we found the modified cTAB procedure used to amplify sweetpotato DNA also amplifies DNA of Fusarium lateritium cultures. DNA banding patterns of infected leaves was compared with those free of the disease. No differences in banding patterns were observed in this preliminary study.

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F.A. Buffone, D.R. LaBonte and C.A. Clark

Chlorotic leaf distortion is a common disease of sweetpotato caused by Fusarium lateritium. This fungus is unique among Fusarium species in that it grows epiphytically on leaves and shoot tips of sweetpotato. Fusarium lateritium mycelia appear as white masses on leaves, and this fungus can cause chlorosis under periods of bright sunlight. When environmental conditions are not favorable for growth, this organism is not readily observed on sweetpotato. The objective of this research was to see if DNA of F. lateritium is amplified using PCR techniques during amplification of sweetpotato DNA. Our results show cTAB extracts of sweetpotato inoculated with F. lateritium have additional bands not present in a control free of F. lateritium. Furthermore, these bands correspond to banding patterns obtained from the F. lateritium isolate DNA when amplified alone. Researchers who use sweetpotato tissue in PCR-based research, e.g., phylogenetic research, should be aware of these amplified products. This situation is further compounded because numerous F. lateritium biotypes are present in the environment.

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Rick D. Peters, Tharcisse Barasubiye and Joanne Driscoll

, the severity of disease development in the storage of concern in this study would indicate that it may need to be considered as part of the spectrum of postharvest pathogens of rutabaga. Fusarium species are ubiquitous in soil and often able to

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Benjamín Moreno, Cristián Jacob, Marlene Rosales, Christian Krarup and Samuel Contreras

a major limitation in watermelon production ( Miguel et al., 2004 ). Yield losses of up to 80% have been reported in watermelon crops affected by this pathogen ( Zhang et al., 2012 ). Fusarium species remain in the soil for years as chlamydospores

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Martin M. Williams II and Carl A. Bradley

and oomycete pathogens, such as Fusarium species, Phytophthora sojae , Pythium species, and Rhizoctonia solani , are not uncommon and caused estimated annual yield losses averaging 11,749,000 Mg in North America grain-type soybean between 2010

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Tomohiro Okada and Yoh-ichi Matsubara

, Fusarium proliferatum (Fp), and Fusarium redolens etc. ( Knaflewski et al., 2008 ; Reid et al., 2002 ; Wong and Jeffries, 2006 ). In Japan, Nahiyan et al. (2011) demonstrated that Foa and Fp are dominant fusarium species in asparagus decline fields

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Juan Pablo Fernández-Trujillo, Gene E. Lester, Noelia Dos-Santos, Juan Antonio Martínez, Juan Esteva, John L. Jifon and Plácido Varó

-isogenic line SC7–2 with hyphae of Cladosporium species and Fusarium species. (D) PI 161375 with the typical gum as a response to the start of cracking. (E) Severe deep furrow in the Korean accession PI 161375. (F) Longitudinal peeling (skin netting) in

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Abu Shamim Mohammad Nahiyan and Yoh-ichi Matsubara

attempted ( Pontaroli and Camadro, 2001 ); however, it takes a long time to develop. On the other hand, biological control of fusarium disease was tried by inoculation with non-pathogenic isolates of the fusarium species ( Blok et al., 1997 ; Reid et al