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Dario J. Chavez and Paul M. Lyrene

blueberry from a cross of diploid and hexaploid species J. Hered. 40 304 306 Hokanson, K. Hancock, J. 2000 Early-acting inbreeding depression in three species of Vaccinium (Ericaceae) Sex. Plant Reprod. 13 145 150 Krebs, S.L. Hancock, J.F. 1990 Early

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Kevin R. Kosola and Beth Ann A. Workmaster

Cranberry and other members of the Ericaceae commonly form ericoid mycorrhizal (ERM) associations with fungi [e.g., Rhizoscyphus ericae (D.J. Read) W.Y. Zhuang and Korf [syn. Hymenoscyphus ericae (D.J. Read) Korf and Kernan] ( Allen et al

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Lisa J. Rowland, Anik L. Dhanaraj, James J. Polashock, and Rajeev Arora

Expressed sequence tag-polymerase chain reaction (EST-PCR) markers for DNA fingerprinting and mapping in blueberry (Vaccinium sp.) had previously been developed from expressed sequence tags (ESTs) produced from a cDNA library, derived from RNA from floral buds of cold acclimated plants. Because EST-PCR markers are derived from gene coding regions, they are more likely to be conserved across populations and species than markers derived from random regions of DNA, such as randomly amplified polymorphic DNA (RAPD) or amplified fragment length polymorphism (AFLP) markers. In this study, we tested whether many of the EST-PCR primer pairs developed for blueberry are capable of amplifying DNA fragments in other members of the family Ericaceae. In addition, we cloned and sequenced a selection of 13 EST-PCR fragments to determine if they showed homology to the original blueberry cDNA clones from which the EST-PCR primer pairs were derived. Closely related cranberry genotypes (two wild selections of V. oxycoccus L. and two cultivars of V. macrocarpon Aiton, `Early Black' and `Stevens') and more distantly related rhododendron genotypes (one wild selection each of Rhododendron arboreum Marsh, R. maximum L., and R. ponticum L. and three complex species hybrids, `Sonata', `Grumpy Yellow', and `Roseum elegans') were used. Of 26 primer pairs tested in cranberry, 23 (89%) resulted in successful amplification and eight of those (35%) amplified polymorphic fragments among the cranberry genotypes. Of 39 primer pairs tested in rhododendron, 29 (74%) resulted in successful amplification and 21 of those (72%) amplified polymorphic fragments among the rhododendron genotypes. Approximately 50% of the 13 sequenced EST-PCR fragments were found to be homologous to the original blueberry cDNA clones. These markers should be useful for DNA fingerprinting, mapping, and assessing genetic diversity within cranberry and rhododendron species. The markers which are shown to be homologous to the blueberry cDNA clones by DNA sequencing should also be useful for comparative mapping and genetic diversity studies between some genera of the family Ericaceae.

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Nian Wang, Zhang Chang Qin, Jun-bo Yang, and Jing-li Zhang

, F.D. Stevens, P. Wallace, G.D. Anderberg, A. 2005 Rhododendron (Ericaceae) 260 455 Wu Z.Y. Raven P.H. Flora of China Vol. 14 Science Press, Beijing, China; Missouri Botanical Garden Press

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Xue-qin Wang, Yuan Huang, and Chun-lin Long

, M.Y. Hu, L.Z. Yang, H.B. Qin, H.N. Min, T.L. David, F. Chamberlain, P.S. Wallace, G.D. Anderberg, A. 2005 Rhododendron (Ericaceae) 333 Wu Z.Y. Raven P

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Carrie A. Radcliffe, James M. Affolter, and Hazel Y. Wetzstein

similar to other members of the Ericaceae ( Hermann and Palser, 2000 ; Hesse, 1983 ; Lu et al., 2009 ; Palser et al., 1992 ; Williams and Rouse, 1990 ; Zomlefer, 1994 ); anthers dehisce longitudinally, and binucleate pollen is borne in tetrads of four

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Paul J. Croft, Mark D. Shulman, and Roni Avissar

Cranberry (Vaccinium macrocarpon Ericaceae Ait.) stomatal conductivity (SC) was investigated in the field to examine plant response as a function of weather conditions. Measurements were made during fruit maturation on 14 days between 0540 and 1710 h r, as weather conditions permitted. SC ranged from 0.02 to 0.08 cm·s-1 and was much lower than for most other crops. Scatter plots of SC vs. leaf temperature by day indicated only a weak linear relationship. When the data were stratified by time of day and by clear and overcast skies, several significant Pearson correlation coefficients suggested a stomatal response. The findings, when combined with current knowledge of the physical structure of cranberry stomata, suggest that cranberries behave as xeromorphic plants.

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He Lisi, Su Jiale, Liu Xiaoqing, Li Chang, and Chen Shangping

bloom and decrease rapidly during fruit maturation ( Yamada et al., 2007 ). The genus Rhododendron , belonging to the family Ericaceae, comprises almost 1000 species with a worldwide distribution but which is concentrated within the sub-alpine and

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S. De Schepper, L. Leus, M. Mertens, E. Van Bockstaele, M. De Loose, P. Debergh, and J. Heursel

Ploidy level was determined for six species and 88 cultivars of the Rhododendron subgenus Tsutsusi. High-resolution flow cytometry of nuclear DNA was performed on macerated plant tissue. All plants analyzed were diploid (2n = 26) with the exception of `Euratom', `Euratom Orange', and `Red Wing', which were triploid (3n = 39), and `Casablanca Tetra', which was found to be a cytochimera: mixoploid (2n + 4n) in the LI and LII, but tetraploid in the LIII. The described method has proven to be useful in screening a large population of rhododendrons. Analysis of different organs and plant tissues was easily accomplished through flow cytometry, and has proven useful in determining the ploidy of different histogenic layers.

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Wayne A. Mackay

Mature flowering Arbutus texana trees were successfully micropropagated from shoot tips. Optimum shoot proliferation was achieved on a basal medium consisting of WPM salts, MS vitamins, and sucrose supplemented with 11.1 or 22.2 μm BA and no auxin. Microcuttings rooted readily when pulsed with 6.1 μm IBA for 1 week and transferred to auxin-free medium. The addition of charcoal to the rooting medium improved root branching and elongation but suppressed root formation. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA); indole-3-butyric acid (IBA).