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Candidatus Liberibacter asiaticus (CLas), the supposed causal agent of Huanglongbing (HLB), is a deadly bacterial pathogen responsible for devastation of the Florida citrus industry. CLas has been detected in nearly all citrus-producing areas in

Candidatus Liberibacter. Of the three species identified so far, only C Las has been found in North America. C Las is vectored by the phloem-feeding psyllid Diaphorina citri ( Halbert and Manjunath, 2004 ) and transmitted into the phloem of citrus leaves

isolation and Candidatus Liberibacter asiaticus quantification. Nucleic acid was extracted from citrus leaf laminar discs (3 mm diameter, ≈11 mg) using REDExtract-N-Amp (Sigma, St. Louis, MO) according to the manufacturer's instructions. Copy number of CLas

until recently ( Sechler et al., 2009 ). Three Candidatus spp. of the pathogen are currently known. The most widespread Asian species, Ca . Liberibacter asiaticus, is found in all HLB-affected countries except Africa. The African species, Ca . L

following infection with the bacterial pathogen Candidatus Liberibacter asiaticus causing huanglongbing in Florida Plant Sci. 175 291 306 Ammar, E.D. Walter, A.J. Hall, D.G. 2013 New excised-leaf assay method to test inoculativity of Asian citrus psyllid

, Candidatus Liberibacter spp. have not been isolated or cultured on artificial media; hence, traditional bacterial quantification through colony-forming units plate counts cannot be used ( Li et al., 2008 ). An estimation of CLas quantitative distribution in

Candidatus Las, a phloem-limited Gram-negative bacterium ( Garnier et al., 1984 ) is the organism associated with citrus HLB in Florida and most other citrus-producing countries around the world ( Bové, 2006 ; Gottwald, 2010 ). HLB, which is also

analysis of variance, so means were compared using Kruskal-Wallis nonparametric analyses. Results and Discussion Candidatus Liberibacter asiaticus. Of the 90 samples of each tissue type analyzed for the presence of CLas, 88% of mature leaves, 69% of flowers

associated with Candidatus Liberibacter asiaticus (CLas), a phloem-limited bacterium ( Garnier et al., 1984 ), which has not yet been successfully grown in pure culture ( Merfa et al., 2019 ), and so remains elusive as the undisputed causal agent. Typical

in the genus Candidatus Liberibacter are the presumed agent causing HLB, but this has not yet been proven due to the unculturable nature of the bacterium ( Merfa et al., 2019 ; Molki et al., 2020 ). In Florida, the pathogen associated with HLB is