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Luping Qu, James Polashock, and Nicholi Vorsa

A very efficient adventitious regeneration (shoot organogenesis) system for cranberry (Vaccinium macrocarpon Ait.) leaves was developed. A basal medium consisting of Anderson's rhododendron salts and Murashige and Skoog's (MS) organics, supplemented with 10.0 μm thidiazuron (TDZ) and 5.0 μm 2ip, was effective for adventitious regeneration from leaves for the five cranberry cultivars tested: `Early Black', `Pilgrim', `Stevens', `Ben Lear', and `No. 35'. Parameters examined included: 1) varying combinations of three plant growth regulators (TDZ, 2ip, and NAA); 2) explant orientation (adaxial vs. abaxial side in contact with the medium); and 3) leaf position relative to the apical meristem from the source plant. Cultivars varied in regeneration frequency, but cultivar × growth regulator interaction was nonsignificant. With optimal treatment conditions, regeneration occurred on more than 95% of the explants, with `Early Black' and `Pilgrim' producing as many as 100 shoot meristems per explant. At all concentrations tested, NAA (as low as 0.1 μm) increased callus formation and significantly reduced regeneration. Emerging adventitious shoots were always observed on the adaxial side of the leaves regardless of explant orientation on the medium. Regeneration was much greater when the abaxial side was in contact with the medium, and was not related to leaf position on the source plants. Elongation of adventitious shoots began ≈2 weeks after transfer to the basal medium without growth regulators. Cuttings of elongated shoots rooted 100% both in vitro in the basal medium and ex vitro in shredded sphagnum moss. The high regeneration efficiency achieved by using this system will be very useful in the application of techniques, such as Agrobacterium- and particle bombardment-mediated transformation. Chemical names used: 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea (thidiazuron, TDZ); N6-(γ-γ-dimethyallylamino) purine (2ip); α-naphthaleneacetic acid (NAA).

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Juanxu Liu, Min Deng, Richard J. Henny, Jianjun Chen, and Jiahua Xie

adjusted to 5.8 with 1 M KOH before autoclaving at 121 °C for 25 min. The plant growth regulator solutions of 6-benzyladenine (BA), N 6 -(2-isopentyl) adenine (2iP), 3-indoleacetic acid (IAA), α-naphthalene acetic acid (NAA), N-phenyl-N′-1,2,3-thiadiazol-5

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Jin Cui, Juanxu Liu, Min Deng, Jianjun Chen, and Richard J. Henny

been micropropagated using shoot tips ( Kane, 2000 ; Miller and Murashige, 1976 ). Shoot tips are inoculated on Murashige and Skoog (MS) medium containing 14.8 μ m N -isopentenylaminopurine (2iP) and 5.7 μ m IAA for establishment (Stage I) and then

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Jin Cui, Juanxu Liu, Jianjun Chen, and Richard J. Henny

°C for 25 min. When the medium temperature dropped to ≈50 °C, filter-sterilized stock solutions of BA, N -(2-Chloro-4-pyridl)- N′ -phenylurea (CPPU), KN, 2iP, TDZ, 2,4-D, or NAA were added into the autoclaved basal medium based on the combinations

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Fatemeh Haddadi, Maheran Abd Aziz, Hossein Kamaladini, and Seyed Ali Ravanfar

shoot tips using zeatin and 2ip as the naturally occurring cytokinins and kinetin as the synthetic cytokinin. Materials and methods Shoot regeneration using leaf explants. Medium used consisted of MS basal salts ( Murashige and Skoog, 1962 ), B 5

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Jenna Sicuranza and Nathaniel A. Mitkowski

regulator treatments were prepared by adding IAA and 2iP to this basal media. Medium I contained 1 mg·L −1 (5.7 μ m ) IAA and 5 mg·L −1 (24.5 μ m ) 2iP; medium II contained 4 mg·L-1 (22.8 μ m ) IAA and 15 mg·L −1 (73.8 μ m ) 2iP ( Meyer, 1982 ). One

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Areej A. Alosaimi, Robert R. Tripepi, and Stephen L. Love

stages of micropropagation. The selected cytokinins, BA, kin, 2iP, TDZ, and mT, were examined mainly for their effects on the number of axillary shoots produced on stem explants. These cytokinins were used at concentrations of 0, 1.1, 2.2, 4.4, or 8.8 µ m

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Jane Kahia, Peter Kanze Sallah, Lucien Diby, Christophe Kouame, Margaret Kirika, Simeon Niyitegeka, and Theodore Asiimwe

explants and 5 mm in length for the hypocotyl and root explants. Each of the explant was cultured singly in a test tube. The composition of the culture media was half strength MS medium supplemented with TDZ at 0.1, 0.5, 1.0, and 1.5 μM; 2iP, kinetin and BA

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J. Kevin Parris, Darren H. Touchell, Thomas G. Ranney, and Jeffrey Adelberg

( Kamenicka and Lanakova, 2000 ). Although several cytokinins have been used to induce shoot proliferation, BAP has been used most often for magnolia. For Magnolia × soulangeana, 1.2 μM BAP was shown to produce greater shoot proliferation than 2iP, kinetin

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Jane Kahia, Margaret Kirika, Hudson Lubabali, and Sinclair Mantell

cultured on half-strength MS media supplemented with 30 mg/L cysteine, 100 mg/L inositol, and 3% (w/v) sucrose. To this media, adenine type of cytokinins (kinetin or 2iP) tested at three concentrations and a control (0, 0.5, 5, or 25 µ m ) and phenylurea