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Joseph P. Albano and William B. Miller

Excised roots of `First Lady' marigold (Tagetes erecta L.) grown in an aerated 0 Fe nutrient solution had Fe(III)-DTPA reductase activity 14-fold greater, and an enhanced ability to acidify the rhizosphere than plants grown in a solution containing 0.018 mm (1 ppm) Fe-DTPA. Reductase activity and rhizosphere acidification of plants grown in 0.018 and 0.09 mm Fe-DTPA were similar. Manganese concentration in leaves of plants grown in the 0 Fe treatment was 2-fold greater than in leaves of plants grown in the 0.018 mm Fe-DTPA treatment. These results indicated that `First Lady' marigold is an Fe-efficient plant that possesses both an inducible or adaptive reductase system and the ability to acidify the rhizosphere, and that these Fe-efficiency reactions do not occur when Fe is sufficient. Chemical name used: ferric diethylenetriaminepentaacetic acid, monosodium salt (Fe-DTPA).

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R.A. Mirabello, A.E. Einert, and G.L. Klingaman

The effects of a mulch material on nutrient availability remain questionable. As organic materials decompose, the increased activity of microorganisms immobilizes nutrients (particularly nitrogen) to preform this process. The decomposition of mulch material and the activity of microorganisms may then compete for nutrients applied to ornamental species in the landscape. To examine this question, four widely available mulch materials (pine bark, cypress pulp, pine straw, and cottonseed hulls) and three fertilizer application methods (granule, liquid, and time release), which were applied either above or below the mulch, were established. Beds with and without mulch cover and no fertilization were established as controls. Marigolds, Tagetes erecta `Hybrid Gold', were planted within the beds. Growth response was found to be greatest in beds with cottonseed hulls. Cottonseed hulls are reported to have a high nitrogen content of their own that may influence less immobilization of nitrogen for decomposition. Beds using pinebark showed significant reduction in plant growth. Fertilization application method also demonstrated significant differences in plant response. The use of a granule fertilizer produced the greatest growth response although initial plant loss was observed in beds using this method. The fast release nature of granule fertilizer and potential toxicity were the suspected reason for this observation. Growth data indicated plant performance was unaffected by fertilizer placement.

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R.M. Mirabello, A.E. Einert, and G.L. Klingaman

The objective of this study was to examine the influence of mulch material and fertilizer application method on nutrient availability in a landscape situation. Beds containing four mulch materials (pine bark, cypress pulp, pine straw, and cottonseed hulls) and three fertilizer application methods (granule, liquid, and time release) were established. Fertilizer placement included application either above or below the mulch horizon. Beds with and without mulch cover and no fertilization were established as controls. Marigolds, Tagetes erecta `Hybrid Gold', were planted within the beds. Plants in unmulched or fertilized control beds had greater dry weights than plants in beds with mulch alone. Only plants grown in the cottonseed hull control demonstrated a slight improvement and cottonseed hulls demonstrated the best plant performance overall. The greater nitrogen content of cottonseed hulls may influence less immobilization of nitrogen in the soil solution during decomposition and reduce competition for nutrients between microorganisms and plants. Fertilization improved plant growth in all treatments except pine bark. Beds using pine bark showed significant reduction in plant dry matter accumulation. Potential toxicity or changes in soil chemistry by pine bark may have influenced these results and will be examined in further experiments. Fertilizer placement had no effect on plant growth.

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Mary C. Halbrooks and Joseph P. Albano

A specific physiological disorder of the recently matured leaves of Tagetes erecta has been demonstrated to be associated with high levels of iron and manganese in affected tissues. In previous work by the authors, the disorder was inducible and increased in severity with increasing levels of iron DTPA supplied to plants grown in peat-based media, but was much less severe when iron DTPA treatments were applied to plants grown hydroponically. At low concentrations of iron DTPA in solution, the occurrence of the disorder was more closely correlated with increased levels of manganese in leaf tissue than iron, Objectives of this study were to determine the effects of iron chelate (DTPA) on occurrence of the disorder and the availability of iron and manganese in the media in the absence of added manganese. Iron DTPA (1, 5, 15, and 20 ppm) was supplied to two cultivars of Tagetes erecta, `Voyager' and `First Lady', grown in a commercial peat-based media product under controlled environmental conditions. Concentrations of iron and manganese in leachate samples taken weekly, and in symptom and non-symptom tissue at harvest, and the progression of the symptoms in leaf tissue over time. will be discussed.

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Theresa Bosma, John Dole, and Niels Maness

Marigold flower pigments can be extracted and used as a natural source of food colorants in the poultry and dairy industry. These pigments impart an orange color to egg yolks and a yellowish color to dairy products. We examined four African marigold cultivars for their ability to be commercially grown and harvested mechanically. `E-1236' yielded the highest quantity of lutein (22 kg/ha), a carotenoid pigment, using a spectrophotometer for quantification. `E-1236' and `A-975' were the earliest flowering cultivars, 11 June 1998 for transplants and 9 July 1998 for direct-seeded, at 8 weeks after sowing regardless of field establishment method. `E-1236' produced the greatest number of flowers in a production season, both as transplants (68 flowers/plant) and direct-seeded (57 flowers/plant) at 363,290 plants/ha. Transplants resulted in two more harvests in a single season than direct-seeded plants. Subsequently, more flowers and petal material were produced for pigment extraction than with direct-seeded plants. A one-time application of ammonium nitrate (28.02 kg/ha) at mid-season did not significantly effect flower number, flower weight, or pigment yield. Experiment was repeated in 1999 with four cultivars, two field establishment methods, seven harvest dates, and five nitrogen applications.

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María de Lourdes Miranda-Ham, Lizbeth A. Castro-Concha, Elide Avilés-Berzunza, and Gregorio Godoy-Hernández

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Joseph P. Albano, William B. Miller, and Mary C. Halbrooks

A specific physiological disorder, bronze speckle (J.P.A.'s nomenclature), was consistently induced in `First Lady' and `Voyager' marigold with Fe-DTPA concentrations greater than 0.018 mm Fe-DTPA (1 ppm) applied to a soilless medium. The disorder was characterized by specific symptomology distinguished visually by speckled patterns of chlorosis and necrosis, and downward curling and cupping of leaves. The percentage of total leaf dry weight affected with symptoms generally increased with increasing Fe-DTPA treatments. Symptomatic leaf tissue had a greater Fe concentration than corresponding asymptomatic leaf tissue. Leaf Mn concentrations in symptomatic and asymptomatic tissue were similar. In `First Lady', older leaf tissue accumulated more total Fe and was associated with more severe symptoms than younger tissue. Media leachate Fe concentrations increased over 6 weeks and were larger at greater Fe-DTPA treatments. Adjustment of nutrient solution pH to 4.0, 5.25, or 6.5 did not alter media pH, nor did it prevent disorder symptoms. Application of Fe-DTPA containing nutrient solution to a soilless medium resulted in leachate Fe levels 3 times greater than for FeSO4 treatments. Chemical names used: ferric diethylenetriaminepentaacetic acid, monosodium salt (Fe-DTPA).

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T.K. Hartz, F.J. Costa, and W.L. Schrader

The study was undertaken to determine the physiochemical properties and nutrient supply characteristics of composted green yard and landscape waste (CGW) and to document its performance as a field soil amendment or constituent of potting media. Three CGW samples were collected from each of two composting operations in California from Nov. 1993 to Apr. 1994. Macronutrient content varied widely between operations, and among samples from the same operation, with mean total N, P, and K levels averaging 1.1%, 0.26%, and 0.67%, respectively. Controlled-environment incubation of a moist 1 CGW: 9 soil blend (2 weeks at 30 °C) was conducted to determine net N mineralization from CGW. Despite low C: N ratios (<12), five of six CGW samples showed net immobilization, a characteristic of immature compost. An in-field incubation of soil amended with 1% or 2% CGW (w/w) showed no net N release from CGW over 4 months. In a field trial, bell pepper (Capsicum annuum L.) fruit yield was increased by soil amendment with CGW (17 or 34 t·ha–1) under a low N fertilizer regime (168 kg·ha–1), but was unaffected where sufficient fertilizer N (280 kg·ha–1) was applied. CGW was compared with peat as a constituent of potting media; both were blended 1:1 (v/v) with perlite and used in the production of tomato (Lycopersicon esculentum Mill.) and marigold (Tagetes erecta L.) plants under varying fertigation regimes (constant feed of N at 0, 50, or 100 mg·L–1 as 15N–13P–12K). CGW was equivalent or superior to peat in plant growth; CGW did contribute to crop macronutrient nutrition, but the highest fertigation rate was required for optimum growth.

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Luis A. Valdez-Aguilar, Catherine M. Grieve, and James Poss

6 Feb. 2007. Plugs of ‘French Vanilla’ (five plugs) ( Tagetes patula L.), ‘Yellow Climax’ (three plugs) ( Tagetes erecta L.), and ‘Flagstaff’ (three plugs) ( Tagetes erecta L.) were transplanted on 8 Mar. 2007 into each of 30 sand tanks containing

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Dennis N. Portz and Gail R. Nonnecke

Li, 2003 ). Sorghum bicolor rotated with strawberry showed low counts of weeds and pathogenic nematodes and fungi ( Elmer, 1999 ; Kratochvil et al., 2004 ; LaMondia et al., 2002 ; McSorley and Gallaher, 1993 ). Tagetes erecta ‘Crackerjack