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Wayne A. Mackay

Micropropagation studies of several desirable species native to west Texas were initiated to develop clonal propagation systems for ornamental production. Actively growing shoots were collected from mature Texas Madrone and Mexican Redbud trees and successfully cultured on basal medium consisting of WPM salts, MS vitamins, 30g·l-1 sucrose, 0.8% Phytagar supplemented with 2.5 mg·l-1 BA. Shoots were subcultured every 4 weeks on the same medium to obtain sufficient culture material for experiments. Experiments were performed examining inorganic salt formulations, growth regulator materials, and gelling agents to optimize shoot proliferation and rooting.

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Wayne A. Mackay

Mature flowering Arbutus texana trees were successfully micropropagated from shoot tips. Optimum shoot proliferation was achieved on a basal medium consisting of WPM salts, MS vitamins, and sucrose supplemented with 11.1 or 22.2 μm BA and no auxin. Microcuttings rooted readily when pulsed with 6.1 μm IBA for 1 week and transferred to auxin-free medium. The addition of charcoal to the rooting medium improved root branching and elongation but suppressed root formation. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA); indole-3-butyric acid (IBA).