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Mark J. Howieson and Nick Edward Christians

raw materials to redevelop leaf and shoot tissue ( Davidson and Mithorpe, 1966 ; Donaghy and Fulkerson, 1998 ; Morvan-Betrand et al., 1999 ). The primary reserve carbohydrate of creeping bentgrass ( Agrostis stolonifera L.) is fructan. Fructan is

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Aneta K. Studzinska, David S. Gardner, James D. Metzger, David Shetlar, Robert Harriman, and T. Karl Danneberger

Creeping bentgrass ( Agrostis stolonifera L.) is a turfgrass species highly suitable for use on golf course tees, greens, and fairways. As a result of its ability to provide exceptional quality playing surfaces when mowed short, it is used

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Edward J. Nangle, David S. Gardner, James D. Metzger, John R. Street, and T. Karl Danneberger

areas of ≈1500 m −2 each were then established in June 2006 with washed creeping bentgrass ‘Penncross’ ( Agrostis stolonifera L. cv. Penncross) sod (H&E Sod Nursery, Momence, IL) and allowed to acclimate for 3 weeks before initiation of routine

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Eric M. Lyons, Robert H. Snyder, and Jonathan P. Lynch

Root distribution in turfgrass systems influences drought tolerance and resource competition with undesirable species. We hypothesized that spatial localization of phosphorus (P) supply would permit manipulation of turfgrass root distribution. To test this hypothesis, creeping bentgrass (Agrostis stolonifera L.) plants were exposed to localized P supply in two experiments. The first experiment split the root zone horizontally into two different growth tubes and the second used alumina-buffered P (Al-P) to localize P availability deeper within a continuous root zone. In the horizontally split root zones, heterogeneous P availability led to no difference in shoot growth compared with uniform P availability. Root proliferation was greatest in the growth tube with available P compared with the growth tube without P. The use of Al-P, regardless of its spatial distribution, doubled root-to-shoot ratios compared with soluble P. Much of the increase in the ratio was accounted for by reduced shoot growth. Use of Al-P increased rooting deeper in the root zone, especially when the Al-P was mixed only in the lower portion of the root zone. Our results are consistent with the hypothesis that root distribution of creeping bentgrass can be manipulated by spatial localization of P supply in the root zone and indicate that relative biomass allocation to roots and shoots may be manipulated with buffered P sources.

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Peter H. Dernoeden, John E. Kaminski, and Jinmin Fu

( Agrostis stolonifera ) control in cool-season turfgrass Weed Technol. 20 340 344 Bevard, D.S. 2007 The rough dilemma in the mid-Atlantic region USGA Green Section Record 45 1 1 6 Bhowmik, P

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Mahalaxmi Veerasamy, Yali He, and Bingru Huang

Heat stress induces leaf senescence and causes changes in protein metabolism. The objective of this study was to investigate effects of exogenous application of a synthetic form of cytokinin, zeatin riboside (ZR), on protein metabolism associated with leaf senescence under heat stress for a cool-season grass species. Creeping bentgrass (Agrostis stolonifera L.) (cv. Penncross) plants were exposed to optimum temperature control (20/15 °C, day/night) and heat stress (35/30 °C) in growth chambers. Before heat stress treatments, foliage was sprayed with 10 μmol ZR or water (untreated) for 3 days and then once per week during 35 days of heat stress. Leaf chlorophyll content, photochemical efficiency (Fv/Fm), and soluble protein content declined, whereas protease activity increased during heat stress. Treatments with ZR helped maintain higher leaf chlorophyll content, Fv/Fm, and soluble protein content under heat stress. Protease activity in ZR-treated plants was lower than that of untreated plants. Zeatin riboside-treated plants had less severe degradation of ribulose-1,5-bisphosphate carboxylase proteins than untreated plants exposed to heat stress. In addition, ZR treatment upregulated the expression of 32- and 57-kDa proteins under heat stress conditions. These results demonstrated that the exogenous application of ZR ameliorated the negative effects of heat stress, as manifested by suppression or delay of leaf senescence. Cytokinins may have helped to alleviate heat stress injury, probably by slowing down the action of protease and by induction or upregulation of heat-shock proteins.

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Stephen E. McCann and Bingru Huang

creeping bentgrass ( Agrostis stolonifera L.) ( DaCosta and Huang, 2006 ), Kentucky bluegrass ( Poa pratensis L.) ( Perdomo et al., 1996 ), and zoysiagrass ( Zoysia japonica Steud.) ( Qian and Fry, 1997 ). Regardless, adaptation of specific plant species

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A. Douglas Brede

‘Alpha’ and ‘T-1’ are two novel cultivars of creeping bentgrass ( Agrostis stolonifera L.) released in Oct. 2004 by Jacklin Seed by Simplot. Creeping bentgrass is a low-growing, stoloniferous monocot used almost exclusively on golf course putting

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Nanqing Liu, Yixin Shen, and Bingru Huang

. 52 1891 1901 Merewitz, E.B. Gianfagna, T. Huang, B. 2011 Photosynthesis, water use, and root viability under water stress as affected by expression of SAG12-ipt controlling cytokinin synthesis in Agrostis stolonifera J. Expt. Bot. 62 383 395 Munns, R

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Yan Xu and Bingru Huang

transport. Cool-season turfgrass species such as Agrostis stolonifera are sensitive to heat stress and experience a series of physiological injuries when exposed to temperatures above 30 °C. Leaf senescence was observed after 20 d at 30 °C and only 8 d at